Neuropsychpharmacological study on the role of the basal forebrain regions for regulation of emotion

基底前脑区情绪调节作用的神经心理药理学研究

• 批准号: 12670964
• 负责人: YOSHIDA Masami
• 金额: $ 2.18万
• 依托单位: Kurume University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12670964/
• 关键词: Emotion; Feeding; Basal forebrain region; Nucleus accumbens; Ventral pallidum; GABA; orexin; 側坐核shell; ドーパミン; ムシモール; Fos蛋白; 脱抑制

We tried to find chemical nature of brain pathways through the basal forbrain area involved in feeding response using both in vivo reverse microdialysis and immunohistochemical techniques. Muscimol, a GABA agonist, injected locally into the nucleus accumbens shell (NACs), caused a significant increase in food intake. This muscimol-induced feeding was accompanied by both marked increases in the micro-dialysate concentration of dopamine of the NACs and Fos-expression in neurons of accumbofugal target areas such as the ventral pallidum (VP), the lateral hypothalamus and the ventral tegmental area. Sequential staining of Fos- and orexin-immunoreactivities revealed that a portion of these hypothalamic neurons were doublelabelled. In contrast, local muscimol injected into the medial VP caused a significant decrease in food intake.These findings show that muscimol in the NACs would disinhibit accumbofugal target neurons (including a portion of A10 neurons and hypothalamic orexin neurons), resulting in an increase in.food intake. This raises the view that basal forebrain regions exert inhibitory influence on the neurons of the hypothalamic center, thereby regulating emotional behavior such as feeding.

我们试图通过在体反向微透析和免疫组织化学技术来寻找通过基底前脑区参与摄食反应的脑通路的化学性质。蝇蕈醇是一种GABA激动剂，局部注射到神经核壳（NAC）中，引起摄食量显著增加。这种蝇蕈醇诱导的喂养伴随着两个显着增加微透析液浓度的多巴胺的NAC和Fos表达的神经元的离体靶区，如腹侧苍白球（VP），下丘脑外侧和腹侧被盖区。Fos和食欲素免疫反应的顺序染色显示，这些下丘脑神经元的一部分是双标记的。这些结果表明，在NAC中的蝇蕈醇可以解除对离体靶神经元（包括部分A10神经元和下丘脑食欲素神经元）的抑制，导致摄食量增加。in.food这就提出了这样一种观点，即基底前脑区域对下丘脑中枢的神经元施加抑制性影响，从而调节进食等情绪行为。

项目成果

E.Kihara, M.Tanaka, M.Yoshida, H.Emoto, H.Ishii: "Effect of ageing on noradrenaline release in the basolateral amygdaloid nucleus of rats exposed to chronic forced walking stress"Biogenic Amines. 16(6). 579-591 (2001)

E.Kihara、M.Tanaka、M.Yoshida、H.Emoto、H.Ishii：“衰老对暴露于慢性强迫行走应激的大鼠基底外侧杏仁核中去甲肾上腺素释放的影响”生物胺。

田中正敏, 吉田眞美, 江本浩幸, 石井秀夫: "ストレスによる脳各部位のノルアドレナリン放出と創薬-不安と関連して-"日薬理誌. 116. 96-100 (2000)

Masatoshi Tanaka、Mami Yoshida、Hiroyuki Emoto、Hideo Ishii：“由于压力和药物发现，大脑各个部位释放去甲肾上腺素 - 与焦虑有关 -”《日本药理学杂志》116. 96-100 (2000)。

Wu, Y.-L, Yoshida, M., Emoto, H., Ishii, H., Koga, K.andTanaka, M.: "Effects of acute and chronic administration of MCI-225, a new selective noradrenaline reuptake inhibitor or with 5-HT3 receptor blocking action, on extracellular noradrenaline levels in

Wu, Y.-L, Yoshida, M., Emoto, H., Ishii, H., Koga, K.和Tanaka, M.：“MCI-225（一种新型选择性去甲肾上腺素再摄取抑制剂或

Tanaka, M., Yoshida, M., Emoto, H. and Ishii, H.: "Noradrenaline systems in the hypothalamus, amygdala and locus coeruleus are involved in the provocation of anxiety : basic studies."Eur. J. Pharmacol.. 405. 397-406 (2000)

Tanaka, M.、Yoshida, M.、Emoto, H. 和 Ishii, H.：“下丘脑、杏仁核和蓝斑中的去甲肾上腺素系统参与焦虑的激发：基础研究。”Eur。

Kihara, E., Tanaka, M.(Yoshida, M. and Emoto, H. ans Ishii, H.: "Effect of aging on noradrenaline release in the basolateral amygdaloid nucleus of rats exposed to chronic forced walking stress."Biogenic Amines. 16. 579-591 (2001)

Kihara, E., Tanaka, M.（Yoshida, M. 和 Emoto, H. ans Ishii, H.：“衰老对暴露于慢性强迫行走应激的大鼠基底外侧杏仁核中去甲肾上腺素释放的影响。”生物胺。