Spectral analysis of renal microcirculation in spontaneously hypertensive rat (SHR) using the new developed wavelet method

使用新开发的小波方法对自发性高血压大鼠（SHR）肾微循环进行频谱分析

• 批准号: 12671055
• 负责人: KOMATSU Kazutoshi
• 金额: $ 2.05万
• 依托单位: Nihon University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671055/
• 关键词: Wavelet method; spontaneously hypertensive rat; stroke prone spontaneously hypertensive rat; tubuloglomerular feedback; Myogenic response; Renal microcirculation; Angiotensin II; Nitric oxide

The kidney exhibits an ability to maintain the blood flow and glomerular filtration rate constant when the renal perfusion pressure is altered. Two factors contribute to this autoregulatory response, tubuloglomerular feedback and myogenic response.Previously, the renal autoregulation was studied using the micropuncture technique. In this study, we investigated the renal cortical blood flow using the spectral analysis (Maximum entropy method; MEM and Wavelet method) to determine the role of nitric oxide and angiotensin II on the renal autoregulation in SHR.Methods: Study 1. The chloralose-anesthetized male WKY/Izm, SHR/Izm and SHRSP/Izm (17 weeks old) were used. Renal homodynamic responses to NO synthesis inhibitor (L-NAME; 0.5, 1.0 mg/kg ia) were studied. Renal cortical blood flow (RBF) was measured by the laser-Doppler flowmeter. The spectral analysis of RBF variability was performed using maximum entropy method. TGF oscillations (30-50mHz) were confirmed by furosemide. Study 2. WKY/I … More zm and SHR/Izm were used. Angiotensin II (Ang II; 5, 15, 50, 100 ng/kg/min, 20-min iv) was infused. RBF was analyzed by Wavelet method.Results: (1) Left ventricle and aorta weights were increased in SHRSP. (2) Baseline mean arterial pressure (MAP) was 131±4, 182±3 and 214±3 mmHg in WKY, SHR and SHRSP, respectively. Baseline RBF was 49±1, 39±2, and 36±1 ml/min/100g in WKY, SHR and SHRSP, respectively. L-NAME increased MAP and decreased RBF. (3) L-NAME significantly increased TGF oscillations in WKY and SHR, but did not affect in SHRSP. (4) Wavelet could analyze RBF simultaneously ever 5 sec. Ang II tended to increased TGF oscillations in both strains. Compared with conventional spectrogram approach based on MEM, wavelet analysis yields a better time-frequency resolution.Conclusions: Tubuloglomerular feedback response was attenuated in SHRSP. Nitric oxide did not play a major role in TGF in SHRSP. This disorder of TGF might be affected by target organ damage. These results suggest that the effects of Nitric oxide on renal autoregulation are impaired in SHRSP. Wavelet method provides improved resolution of analysis and can be used as general indexes of TGF oscillations in rats. Less

当肾脏灌注压改变时，肾脏表现出维持血流量和肾小球滤过率恒定的能力。两个因素有助于这种自我调节反应，管球反馈和肌源性反应。在这项研究中，我们研究了肾皮质血流频谱分析（最大熵法; MEM和小波方法），以确定一氧化氮和血管紧张素II在SHR的肾脏自动调节的作用。选用氯醛糖麻醉的雄性WKY/Izm、SHR/Izm和SHRSP/Izm（17周龄）。用一氧化氮合成抑制剂（L-NAME; 0.5、1.0mg/kg，ia）对大鼠肾血流动力学进行了研究。采用激光多普勒血流仪测定肾皮质血流量。采用最大熵方法对RBF变异性进行谱分析。通过呋塞米证实TGF振荡（30- 50 mHz）。研究2. WKY/I ...更多信息 zm和SHR/Izm。输注血管紧张素II（Ang II; 5、15、50、100 ng/kg/min，20 min iv）。结果：（1）SHRSP大鼠左心室和主动脉重量增加。(2)WKY、SHR和SHRSP的基线平均动脉压（MAP）分别为131±4、182±3和214±3 mmHg。WKY、SHR和SHRSP的基线RBF分别为49±1、39±2和36±1 ml/min/100 g。L-NAME增加MAP，降低RBF。(3)L-NAME显著增加WKY和SHR的TGF-β振荡，但对SHRSP无影响。(4)小波可以同时分析RBF神经网络，每5秒一次。在两种菌株中，Ang II倾向于增加TGF振荡。与传统的基于最大熵原理的频谱分析方法相比，小波分析具有更好的时频分辨率。一氧化氮在SHRSP的TGF中不起主要作用。这种TGF-β的紊乱可能与靶器官损伤有关。提示一氧化氮对SHRSP肾脏自身调节功能的影响受损。小波分析方法提高了分析分辨率，可作为大鼠TGF振荡的综合指标。少

项目成果

小松一俊: "一酸化窒素阻害時の脳卒中易発症高血圧自然発症ラットの尿細管糸球体フィードバック -周波数解析での検討-"Hypertens Res. 24,1. A8 (2000)

Kazutoshi Komatsu：“一氧化氮抑制期间易发生中风的自发性高血压大鼠的肾小球反馈 - 通过频率分析进行检查”Hypertens Res 24,1。

K. Komatsu et al.: "Analysis of heart rate variability during exercise using the new developed wavelet method"Jpn, Circ.. J65 supple I. 408 (2001)

K. Komatsu 等人：“使用新开发的小波方法分析运动期间的心率变异性”Jpn，Circ.. J65 suple I. 408 (2001)

小松一俊: "一酸化窒素阻害時の脳卒中易発症高血圧自然発症ラットの尿細管糸球体フィードバック-周波数解析での検討-"Hypertens Res. 24, 1. A8 (2001)

Kazutoshi Komatsu：“一氧化氮抑制期间易发生中风的自发性高血压大鼠的肾小球反馈 - 通过频率分析进行调查”Hypertens Res 24, 1. A8 (2001)

K. Komatsu: "Effects of nitric oxide inhibitor on tubuloglomerular feedback in spontaneously hypertensive rat: A spectral analysis approach"J Hypertension. 18 (supple 4). S217 (2000)

K. Komatsu：“一氧化氮抑制剂对自发性高血压大鼠肾小球反馈的影响：光谱分析方法”J Hypertension。

小松一俊: "一酸化窒素阻害時の高血圧自然発症ラットの尿細管糸球体フィードバック・周波数解析での検討"Jpn, Circ. J.. 64 supple I. 355 (2000)

Kazutoshi Komatsu：“一氧化氮抑制过程中自发性高血压大鼠的肾小球反馈和频率分析”Jpn，Circ. 64 suple I. 355 (2000)