Liver transplantation from non-heart-beating donor with per fusion prese rvation

无心跳供体肝移植并保留融合

• 批准号: 12671176
• 负责人: NAGAO Takeshi
• 金额: $ 1.92万
• 依托单位: Tokyo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671176/
• 关键词: liver transplantation; non-heart-beating donor; per fusion preservation; pulsatile pump; non-pulsatile pump; 灌留保存法; 灌流保存

1. Orthotopic liver transplantation using pigs (body weight 20-25kg) was carried out.2. Blood was released from donor pig through the catheter inserted into the aorta to keep the systolic blood pressure about GOmmHg. After further release of blood causing cardiac arrest, the liver was harvested as a graft.3. The graft liver was preserved with continuous per fusion preservation technique for 2 hours, using a non-pulsatile pump or a pulsatile pump.4. After preservation, the graft was transplanted to a recipient pig.5. Survival rate, blood chemistry and histological findings ware compared between non-pulsatile pump group and pulsatile pump group.6. The survival rate and histological findings of non-pulsatile pump groupwere superior to those of pulsatile pump group.7. There was no significant difference in the results of blood chemistry between two groups.8. In conclusion, continuous per fusion preservation using non-pulsatile pump is better than that using pulsatile pump.

1. 采用体重20 ~ 25kg的猪进行原位肝移植。供体猪经导管插入主动脉放血，使收缩压维持在5mg左右。在血液进一步释放导致心脏骤停后，肝脏被移植。3 .使用无搏动泵或搏动泵，采用连续融合保存技术保存移植肝2小时。保存后，将移植物移植到受体猪身上。比较无搏动泵组与搏动泵组的生存率、血液化学及组织学检查结果。无搏动泵组的生存率和组织学表现均优于搏动泵组。两组患者血液化学指标比较，差异无统计学意义。综上所述，使用非脉动泵比使用脉动泵更能实现连续的融合保存。

项目成果

鳴海 康方: "心停止後摘出肝保存における低温持続灌流保存法の基礎的研究"移植. 37(6). 267-274 (2002)

Yasukata Narumi：“心脏骤停后肝脏保存的低温连续灌注保存方法的基础研究”移植37（6）。

Y. Narumi: "A comparison between nonpulsatile and pulsatile machchine per fusion preservation in liver transplantation from non-heart-beating donors"Jap J Transplant. 37(6). 267-274 (2002)

Y. Narumi：“非搏动性和搏动性机器在无心跳供体肝移植中融合保存的比较”Jap J Transplant。

内山 正美: "心停止ドナーからの肝移植における低温持続灌流保存法の有用性とpentoxifyllineの効果"東京医科大学雑誌. 58(6). 743-756 (2000)

内山雅美：“低温连续灌注保存方法的实用性和己酮可可碱在心脏骤停供体肝移植中的作用”，东京医科大学杂志 58（6）（2000）。

M.Uchiyama: "Comparison between nonpulsatile and pulsatile machine perfusion preservation in liver transplantation from non-heart beating donors"Transplant Proc. 33. 936-938 (2001)

M.Uchiyama：“非搏动性和搏动性机器灌注保存在无心跳供体肝移植中的比较”Transplant Proc。

M. Uchiyama: "Usefulness of preservation method by Machineper fusion and pentoxifylline on the liver transplantationfrom non-heart-beating donor"J Tokyo Med Univ. 58(6). 743-756 (2000)

M. Uchiyama：“Machineper融合和己酮可可碱保存方法对无心跳供体肝移植的有用性”东京医科大学学报。