Liver transplantation from non-heart-beating donor with per fusion prese rvation

1.采用猪（体重20- 25 kg）进行原位肝移植.通过插入主动脉的导管从供体猪释放血液，以保持收缩压约为100 mmHg。在进一步释放血液引起心脏骤停后，收获肝脏作为移植物。采用非搏动泵或搏动泵连续灌注保存技术保存供肝2 h.保存后，将移植物移植到受体猪体内.比较非搏动泵组和搏动泵组的存活率、血液生化和组织学改变.非搏动泵组的存活率和组织学表现均优于搏动泵组，差异有上级.两组血生化结果无显著性差异.结论：非搏动泵持续灌注保存优于搏动泵。

1. Orthotopic liver transplantation using pigs (body weight 20-25kg) was carried out.2. Blood was released from donor pig through the catheter inserted into the aorta to keep the systolic blood pressure about GOmmHg. After further release of blood causing cardiac arrest, the liver was harvested as a graft.3. The graft liver was preserved with continuous per fusion preservation technique for 2 hours, using a non-pulsatile pump or a pulsatile pump.4. After preservation, the graft was transplanted to a recipient pig.5. Survival rate, blood chemistry and histological findings ware compared between non-pulsatile pump group and pulsatile pump group.6. The survival rate and histological findings of non-pulsatile pump groupwere superior to those of pulsatile pump group.7. There was no significant difference in the results of blood chemistry between two groups.8. In conclusion, continuous per fusion preservation using non-pulsatile pump is better than that using pulsatile pump.