Effect of tension-stress on gene expression of one and cartilage cells. Molecular mechanism of distraction osteogenesis

张力应激对软骨细胞基因表达的影响。

• 批准号: 12671413
• 负责人: YASUI Natsuo
• 金额: $ 2.43万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671413/
• 关键词: Mechanical stress; Distraction osteogenesis; Bone formation; Chondrocytes

We have recently established a rat model of distraction osteogenesis, and demonstrated that the mode of ossification changed from endochondral to intramembranous via transchondroid bone formation depending on the stage of distraction. The present -study was designed to investigate the effect of mechanical tension-stress on gene expression of bone morphogenetic protein (BMP)-2 and BMP-4 and Runx2 by the cells involved in distraction osteogenesis. Runx2 is a transcription factor that exists in the downstream of BMPs. It bind to DNA through Runt- domain and plays an fundamental role in osteoblast differentiation. Northern blot analysis and in situ hybridization showed that Runx2 gene, as well as BMP-2 and BMP-4 genes, were highly expressed by the chondroid cells exposed to mechanical tension-stress. These cells seemed to switch their collagen phenotype from type II to type I to differentiate from chondroid cells into osteoblasts. After completion of distraction, none of BMP-2, BMP-4 and Runx2 were detected withm the lengthened segment. The present results suggest that abundant gene products of BMP-2, BMP-4 could induce abundant Runx2 and enhance the in situ bone formation by paracrine and autocrine mechanism.

我们最近建立了一个大鼠牵张成骨模型，并证明了成骨模式从软骨内到膜内通过transchondroidoid骨形成取决于牵张的阶段。本研究旨在探讨牵张应力对牵张成骨细胞骨形态发生蛋白（BMP）-2、BMP-4和Runx 2基因表达的影响。Runx 2是存在于BMP下游的转录因子。它通过Runt结构域与DNA结合，在成骨细胞分化中起重要作用.北方杂交和原位杂交结果显示，Runx 2基因、BMP-2基因和BMP-4基因在机械应力作用下的软骨样细胞中高表达。这些细胞似乎将其胶原表型从II型转变为I型，从而从软骨样细胞分化为成骨细胞。牵张结束后，在延长段内未检测到BMP-2、BMP-4和Runx 2。本研究结果提示，BMP-2、BMP-4基因产物通过旁分泌和自分泌机制诱导Runx 2基因表达，促进原位骨形成。

项目成果

Yasui N, et al.: "Congenital transverse deficiency of the tibia and fibula : a report of two cases"Skeletal Radiol. 29. 243-246 (2000)

Yasui N 等人：“先天性胫骨和腓骨横向缺陷：两例报告”Skeletal Radiol。

Yasui N.: "Congenital transverse deficiency of the tibia and tibula : a report of two cases"Skeletal Radiol. 29. 243-246 (2000)

Yasui N.：“先天性胫骨和胫骨横向缺陷：两例报告”骨骼放射学。

Yasui N, et al.: "A technique of percutaneous multidrilling osteotomy for limb lengthening and deformity correction"J Orthop Science. 5. 104-107 (2000)

Yasui N 等人：“一种用于肢体延长和畸形矫正的经皮多重钻孔截骨术”J Orthop Science。

Yasui N: "Orthofix External Fixation in Trauma and Orthopaedics"Springer. 18-24 (2000)

Yasui N：“Orthofix 外固定在创伤和骨科中的应用”施普林格。

Yasui N.: "Bifocal lengthening"Orthofix External Fixation in Trauma and Orthopaedics Springer (eds : de Bastiani G, Apley AG, Goldberg A.). 478-484 (2001)

Yasui N.：“双焦点延长”Orthofix 外固定在创伤和骨科 Springer 中的应用（编辑：de Bastiani G、Apley AG、Goldberg A.）。