Musculo-skeletal regeneration during distracionosteogenesis

牵引成骨过程中的肌肉骨骼再生

• 批准号: 18390418
• 负责人: YASUI Natsuo
• 金额: $ 10.87万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18390418/
• 关键词: distraction osteogenesis; callus distraction; limb lenethening; osteoactivin; MMP-3; transgenic mice; bone resorption; bone formation; chondromodulin; bone remodeling; tension-stress effect; 骨延長; 仮骨; ノックアウトマウス; 筋再生; callotasis; 仮骨延長術; メカニ

Distraction osteogenesis is a useful technique to study the response of bone to mechanical tension-stress in vivo. Using this technique, we here showed the unique response of bone to mechanical stress, especially the association between osteoactivin and MMP-3 expression. At 1 week after osteotomy of tibiae (the lag phase), mice were divided into two groups : the distraction osteogenesis group and non-distraction control group. In the first group, the tibiae were lengthened for 2 weeks (the distraction phase), followed by sustaining the length for 5 weeks (the consolidation phase). At 1 week after surgery, fibroblast-like cells (possible progenitors of osteoblasts or chondrocytes), which mainly expressed osteoactivin, were infiltrated into the osteotomy site. Interestingly, osteoactivin- and MMP-3-double positive fibroblast-like cells were accumulated in tibiae during the distraction phase. Consistent with immunohistochemical analysis, the levels of osteoactivin and MMP-3 transcripts in … More tibiae were significantly increased by osteotomy and distraction, respectively, in the distraction osteogenesis group, whereas no upregulated expression of MMP-3 was observed in the non-distraction group. To elucidate this difference in MMP-3 expression, we examined the shedding of osteoactivin during the distraction phase. Extracellular and intracellular fragments of osteoactivin were produced in distraction osteogenesis group, while they were hardly detected in the non-distraction group. These findings suggest that the shedding of osteoactivin is necessary for upregulation of MMP-3 expression in the lengthened segment. Finally, to address the role of osteoactivin-mediated MMP-3 expression in bone, osteoactivin-transgenic mice were subjected to this model. In osteoactivin-transgenic mice, bone resorption in the remodeling zone was significantly inhibited, compared with wild-type mice, whereas bone formation was not altered. We conclude that the shedding of osteoactivin functions as a mechanical tension-stress respondent inducer of MMP-3 for the fibroblast-like cells, and it mediates the inhibition of distracted callus resorption in the lengthened segment. Less

牵张成骨技术是研究骨组织对机械应力-张力反应的一种有效方法。利用这项技术，我们在这里显示了骨对机械应力的独特反应，特别是骨激活素和MMP-3表达之间的关联。在胫骨截骨后1周（滞后期），将小鼠分为两组：牵张成骨组和非牵张对照组。第一组先延长2周（牵引期），再维持5周（固定期）。术后1周，主要表达骨激活素的成纤维细胞样细胞（可能是成骨细胞或软骨细胞的祖细胞）浸润到截骨部位。有趣的是，骨激活素和MMP-3双阳性成纤维细胞样细胞在牵张阶段在胫骨中积累。与免疫组化分析一致，骨激活素和MMP-3转录物在骨组织中的表达水平， ...更多信息 在牵张成骨组中，截骨术和牵张分别显著增加了MMP-3的表达，而在非牵张组中未观察到MMP-3的表达上调。为了阐明MMP-3表达的差异，我们研究了骨激活素在牵张阶段的脱落。牵张成骨组有骨激活素的细胞外和细胞内片段产生，而非牵张成骨组几乎没有骨激活素的细胞外和细胞内片段产生。这些结果表明，脱落的骨激活素是必要的MMP-3的表达在延长段的上调。最后，为了阐明骨激活素介导的MMP-3表达在骨中的作用，对骨激活素转基因小鼠进行该模型。在骨激活素转基因小鼠中，与野生型小鼠相比，重塑区的骨吸收被显著抑制，而骨形成没有改变。我们的结论是，骨激活素脱落的功能作为一个机械的张力-应力响应诱导剂的MMP-3的成纤维细胞样细胞，它介导的延长段中的牵引骨痂吸收的抑制。少

项目成果

Evaluation and correction of the deformities in lower limb in children(review)

儿童下肢畸形的评估与矫正（综述）

• 发表时间: 2007
• 期刊: Journal of Japanese Pediatric Orhtopaedics Society 16(2)
• 作者: Yasui;N;Kawasaki;Y
• 通讯作者: Y

小児膝関節近傍の下肢変形に対する治療

儿童膝关节附近下肢畸形的治疗

• 发表时间: 2007
• 作者: 川崎 賀照;安井 夏生
• 通讯作者: 安井 夏生

JRAB/MICAL-L2 Is Junctional Rab13-binding Protein Mediating the Endocytic Recycling of Occludin.

JRAB/MICAL-L2 是介导 Occludin 内吞再循环的连接 Rab13 结合蛋白。

• 发表时间: 2006
• 期刊: Molecular Biology of the Cell 17
• 作者: Tomoaki Kato;et. al.;Yonehiro Kanemura;Kubo T;Takahashi M;Suzue N;Goto T;Matsui Y;Terai T
• 通讯作者: Terai T

下肢長不等に対する手術:延長術+矯正術

下肢长度不均手术：延长+矫正

• 发表时间: 2007
• 作者: 川崎 賀照;安井 夏生
• 通讯作者: 安井 夏生

Spl Family of Transcription Factors Regulates the Human α2(XI) Collagen Gene(COL11A2)in Saos-2 Osteoblastic Cells.

Spl 转录因子家族调节 Saos-2 成骨细胞中的人类 α2(XI) 胶原蛋白基因 (COL11A2)。

• 发表时间: 2006
• 期刊: J Bone Miner Res 21(5)
• 作者: Takenouchi T;Setoguchi T;Yone K;Komiya S;Suzue N;Goto T
• 通讯作者: Goto T