An investigation on the effect of FGF-2 for the induction of chondrogenesis in full-thickness articular cartilage defects.

FGF-2 对全层关节软骨缺损诱导软骨形成作用的研究。

• 批准号: 12671426
• 负责人: MIZUTA Hiroshi
• 金额: $ 1.92万
• 依托单位: Kumamoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671426/
• 关键词: fibroblast growth factor; full-thickness defect; repair; mesenchymal stem cell; cell migration; chondrogenesis; fibroblast growth factor2; clearance; chemotaxis; fibroblast growth factor-2; PCNA; short term treatment

We investigated the effect of shorter exposure of fibroblast growth factor (FGF)-2 on the repair of die fufl-thickness defects of articular cartilage of rabbits. Five-mm full-thickness articular cartilage defects, which are not repaired spontaneously, were created in the femoral trochlea, and FGF-2 (150 pg/h) was administered into the joint cavity with an osmotic delivery system for 1 day, 3 days, 1 week or 2 weeks. By the treatment with FGF-2 for only 1 day, the defects were resurfaced wife cartilaginous matrix as successfully as those treated for 2 weeks. Immunohistochemical analyses using anti-proliferating cell nuclear antigen demonstrated high proliferative activity of mesenchymal cells in the reparative tissues at 1 week in all FGF-2 treated groups. Clearance of FGF-2 administered into the defect was monitored by a radiotope study. Half-life of FGF-2 in the defect cavity was estimated to be 【less than or equal】 30 min. We also examined in vitro the FGF-2 action on the migration of rabbit marrow-derived mesenchymal cells and found a chemotactic response of those cells to FGF-2. These results indicate that FGF-2 signals participate in mobilization and recruitment of actively replicating mesenchymal cells from bone marrow into the defect cavities.

本实验观察了成纤维细胞生长因子-2短时间暴露对兔关节软骨全层缺损修复的影响。在股骨滑车内造成5 mm非自发修复的全层关节软骨缺损区，关节腔内注射成纤维细胞生长因子-2(150pg/h)，给药1d、3d、1周或2周。仅用成纤维细胞生长因子-2处理1d，就能成功地修复软骨基质缺损区，与用药2周后相同。抗增殖细胞核抗原免疫组织化学检测结果显示，1周时，所有FGF2处理组修复组织中间充质细胞均有较高的增殖活性。放射显微镜研究监测了应用于缺损处的成纤维细胞生长因子-2的清除情况。在缺损区的半衰期估计为[小于或等于]30分钟。我们还在体外检测了成纤维细胞生长因子-2对兔骨髓间充质细胞迁移的作用，发现这些细胞对成纤维细胞生长因子-2有趋化反应。这些结果表明，成纤维细胞生长因子-2信号参与了骨髓间充质细胞主动复制进入缺损腔的动员和募集。