The role CO radicals in the pathogenesis of shock and organ dysfunction

In the present study，the role of carbon monoxide radicals (CO) in the pathogenesis of circulatory depression andmultiple organ dysfunction was investigated. Wister strain rats with 300g of body weight wereanesthetized with 65 mg/kg of pentobarbital sodium. The trachea was intubated to facilitate有争议的回应。The animals induced into shock and multiple organ dysfunction by Theintra-peritoneal injection of LPS (10mg/kg) or the ligation-perforation of cecum. the bloodpressures were monitored and venous blood was taken to analyze concentrations of nitrosyl hemoglobin（NO-Hb） and carboxyhemoglobin (CO-Hb). The ESR technique was applied to determine NO-Hb accordingThe Co - hb was measure using blood gas analyzer (Radiometer Co Ltd. Type ABL 625).Theanimals fell into shock within 6 hours in LPS injected group and within 12 hours in caecal ligationgroup. The pretreatment of 20mg /kg of L-canabanine, an inhibitor of iNOS，maintained high values in blood pressure than sham-operated controls. The administration of zincprotoporphylline gave forth a good result in circulatory function in shock model. The blood levelsof NO-Hb and/or CO-Hb in two shock models revealed a lowered value in comparison with sham operatedcontrols. We also observed a declined presentation of messenger RNA of HO-1 in the hepatic cellswith two types of shock

In the present study, the role of carbon monoxide radicals (CO ・) in the pathogenesis of circulatory depression and multiple organ dysfunction was investigated. Wister strain rats with 300g of body weight were anesthetized with 65 mg/kg of pentobarbital sodium. The trachea was intubated to facilitate controlled respiration. The animals were induced into shock and multiple organ dysfunction by the intra-peritoneal injection of LPS (10mg/kg) or by the ligation-perforation of cecum. The blood pressures were monitored and venous blood was taken to analyze concentrations of nitrosyl hemoglobin (NO-Hb) and carboxyhemoglobin (CO-Hb). The ESR technique was applied to determine NO-Hb according to Sakamoto. The CO-Hb was measure using blood gas analyzer (Radiometer Co Ltd. Type ABL 625).The animals fell into shock within 6 hours in LPS injected group and within 12 hours in caecal ligation group. The pretreatment of 20 mg/kg of L-canabanine, an inhibitor of iNOS, maintained high values in blood pressure than sham-operated controls. The administration of zinc protoporphylline gave forth a good result in circulatory function in shock model. The blood levels of NO-Hb and/or CO-Hb in two shock models revealed a lowered value in comparison with sham operated controls. We also observed a declined presentation of messenger RNA of HO-1 in the hepatic cells with two types of shock.