Study on the role of heat shock protein 27 in the process of development and regeneration of dental pulp

热休克蛋白27在牙髓发育再生过程中的作用研究

• 批准号: 12671765
• 负责人: OHSHIMA Hayato
• 金额: $ 2.5万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671765/
• 关键词: Heat shock protein (Hsp) 25; 27; Dental pulp; Odontoblasts; Tooth replantation; Cavity preparation; Tooth development; Regeneration; Rat; 象牙質形成; ラット

The present study aims to clarify the functional significance of heat shock protein (Hsp) 25/27 during tooth development and pulpal regeneration. The present study demonstrated that Hsp 25/27 was expressed exclusively in fully-differentiated odontoblasts during tooth development. For the observation of pulp regeneration, Wistar rats, 4-week-old and 100-day-old, were used for tooth replantation and cavity preparation, respectively. Cavity preparation caused an edematous reaction between the injured odontoblasts and predentin, but the immunoreaction for Hsp 25/27 remained in the injured odontoblasts. Subsequently, Hsp 25/27-immunoreactivity disappeared in the degenerated odontoblast layer after 12 hours. On postoperative 3 days, newly differentiated odontoblasts replaced the degenerated odontoblasts, and became to exhibit the immunoreaction for Hsp 25/27. Tooth replantation also caused the disappearance of Hsp 25/27-immunopositive cells at the initial stages. On postoperative 5days, plump cells with clear nucleoli at the dentin-pulp border became to show Hsp 25/27-immureactivity. These findings indicate that newly differentiated odontoblasts acquire the immunoreaction for Hsp 25/27 in the regenerated pulpal tissue after both cavity preparation and tooth replantation. There was no Hsp 25/27-immunopositive cell along the pulp-dentin border in the case of bone-like tissue formation in the pulp space following tooth replatation. Thus, the alignment of Hsp 25/27-immunopostive cells along the pulp-dentin border is suggestive of the decisive factor to induce the reparative dentin formation after tooth replantation.

本研究旨在阐明热休克蛋白25/27在牙齿发育和牙髓再生过程中的功能意义。本研究表明，热休克蛋白25/27只表达在完全分化的成牙本质细胞在牙齿发育过程中。为观察牙髓再生情况，选用4周龄和100日龄Wistar大鼠，分别进行牙再植和窝洞制备。窝洞制备后，成牙本质细胞与前牙本质间出现水肿反应，但成牙本质细胞内仍有Hsp 25/27的免疫反应。12小时后，退变的成牙本质细胞层中Hsp 25/27免疫反应消失。术后3天，新分化的成牙本质细胞取代了退化的成牙本质细胞，并开始表现出热休克蛋白25/27的免疫反应。再植牙早期Hsp 25/27免疫阳性细胞消失。术后5d，牙本质-牙髓交界处细胞饱满，核仁清晰，呈Hsp 25/27免疫活性。这些结果表明，新分化的成牙本质细胞获得Hsp 25/27的免疫反应在再生牙髓组织洞制备和牙齿再植。在牙髓间隙骨样组织形成的病例中，牙髓-牙本质边缘沿着无Hsp 25/27免疫阳性细胞。因此，Hsp 25/27免疫阳性细胞沿着牙髓-牙本质边界排列是诱导再植牙修复性牙本质形成的决定性因素。

项目成果

Ohshima, H: "Alteration in the expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp of rat molars following tooth replantation"Archives of Histology and Cytology. 64. 425-437 (2001)

Ohshima, H：“牙齿再植后大鼠磨牙牙髓中热休克蛋白 (Hsp) 25-免疫反应性表达的改变”组织学和细胞学档案。

Ohshima, H.: "Responses of odontoblasts to cavity preparation in rat molars as demonstrated by immunocytochemistry for heat shock protein (Hsp) 25"Archives of Histology and Cytology. 64 (5). 493-501 (2001)

Ohshima, H.：“通过热休克蛋白 (Hsp) 25 的免疫细胞化学证明，成牙本质细胞对大鼠磨牙空洞制备的反应”组织学和细胞学档案。

Ohshima, H.: "Expression of heat shock protein (Hsp) 25-immunoreactivity in the dental pulp and enamel organ during odontogenesis in the rat molar"Connective Tissue Research. (in press). (2002)

Ohshima, H.：“大鼠磨牙成牙过程中牙髓和牙釉质器官中热休克蛋白 (Hsp) 25-免疫反应性的表达”结缔组织研究。

Ohshima, H.: "Quintessence, Year Book, A Guide for Today's Treatment '01"Quintessence Publishing Co.. 522 (2001)

Ohshima, H.：“Quintessence，年鉴，今日治疗指南 01”Quintessence Publishing Co.. 522 (2001)

Ohshima,H: "Transient expression of heat shock protein (Hsp) 25 in the dental pulp and enamel organ during odontogenesis in the rat incisor"Archives of Histology and Cytology. 63・4. 381-395 (2000)

Ohshima，H：“大鼠门牙牙髓和牙釉质器官中热休克蛋白（Hsp）25 的瞬时表达”组织学和细胞学档案 63・4（2000）。