Possible role of immunocompetent cells and the expression of Hsp25 in the process of pulpal regeneration

免疫活性细胞和Hsp25表达在牙髓再生过程中的可能作用

• 批准号: 14571727
• 负责人: OHSHIMA Hayato
• 金额: $ 1.92万
• 依托单位: NIIGATA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2002
• 资助国家: 日本
• 起止时间: 2002 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-14571727/
• 关键词: Dental Pulp; Regeneration; Low Molecular Weight Heat Shock Protein; Antigen-presenting cells; Cavity Preparation; Replantation; Odontoblasts; Immunohistochemistry; 低分子熱ショック蛋白Hsp25

The purpose of the present study was to clarify the relationship between the chronological changes of immunocompetent cells and the expression of heat shock protein(Hsp) 25 in the process of pulpal regeneration after tooth injury in rat molars by immunocytochemistry for Hsp 25 and class II major histocompatibility complex(MHC) antigen. An intense Hsp 25-immunoreactivity was found in the differentiated odontoblasts. Both cavity preparation and tooth replantation caused the degeneration of the odontoblast layer to result in the loss of Hsp 25-immunoreactions in the suffered dental pulp at the early stages after tooth injury. Numerous class II MHC-positive cells appeared along the pulp-dentin border and extend their cell processes into the dentinal tubules at 12-24 hours after cavity preparation and 3 days after tooth replantation. Newly differentiated odontoblast-like cells with Hsp 25-immunoreactivity were arranged at the pulp-dentin border, and the class II MHC-positive cells retreated … More towards the subodontoblastic layer by postoperative 3-5 days after tooth injury. Thus, the common cellular events occur during pulpal regeneration following two different experimental injuries. These findings indicate that the time course of changes in the expression of Hsp 25-immunoreactivity reflects the degeneration/regeneration process of odontoblasts and that the temporal appearance of the class II MHC-positive cells at the pulp-dentin border suggests their participation in odontoblast differentiation as well as in initial defense reactions during the pulpal regeneration process. In the case of laser ablation, on the other hand, distinct abscess formation consisting of polymorphonuclear leukocytes was found in the dental pulp by 3-5 days postoperation. The penetration of masses of oral bacteria was recognizable in the dentinal tubules beneath the prepared cavity. These findings indicate that cavity preparation by laser ablation induces remarkable inflammation by continuous bacterial infections via dentinal tubules. Less

本研究旨在通过对热休克蛋白（Hsp）25和主要组织相容性复合体（MHC）Ⅱ类抗原的免疫细胞化学研究，探讨大鼠磨牙牙体损伤后牙髓再生过程中免疫活性细胞的时序性变化与Hsp 25表达的关系。在分化的成牙本质细胞中发现了强烈的Hsp 25免疫反应。牙外伤后早期，无论是洞腔预备还是再植牙，均引起成牙本质细胞层的变性，导致牙髓组织中Hsp 25免疫反应丧失。在窝洞制备后12-24 h和再植后3 d，牙髓-牙本质边缘出现大量沿着分布的II类MHC阳性细胞，其细胞突起延伸至牙本质小管。新分化的成牙本质细胞样细胞排列于牙髓-牙本质交界处，Ⅱ类MHC阳性细胞消退 ...更多信息 术后3-5天，牙体细胞向成牙本质细胞下层迁移。因此，常见的细胞事件发生在两种不同的实验损伤后的牙髓再生过程中。这些研究结果表明，热休克蛋白25-免疫反应性的表达变化的时间过程反映了成牙本质细胞的变性/再生过程，并认为在牙髓牙本质边界的第二类MHC阳性细胞的时间外观表明他们参与成牙本质细胞分化以及在牙髓再生过程中的初始防御反应。另一方面，在激光消融的情况下，在术后3-5天在牙髓中发现由多形核白细胞组成的明显脓肿形成。口腔细菌的质量渗透是可识别的牙本质小管下面的准备腔。这些发现表明，通过激光烧蚀的洞制备通过经由牙本质小管的连续细菌感染诱导显著的炎症。少

项目成果

H.Ohshima, K.Nakakura-Ohshima, K.Takeuchi, M.Hoshino, Y.Takano, T.Maeda: "Pulpal regeneration after cavity preparation, with special reference to close spatio-relationships between odontoblasts and immunocompetent cells."Microsc.Res.Tech.. 60(5). 483-490

H.Ohshima、K.Nakakura-Ohshima、K.Takeuchi、M.Hoshino、Y.Takano、T.Maeda：“空腔制备后的牙髓再生，特别是成牙本质细胞和免疫活性细胞之间的密切空间关系。”Microsc。

Suzuki, T.他3名: "An immunocytochemical study of pulpal responses to cavity preparation by laser ablation in rat molars using antibodies to heat shock protein (Hsp) 25 and class II MHC antigen"Cell and Tissue Research. 315・3. 311-319 (2004)

Suzuki, T. 和其他 3 人：“使用热休克蛋白 (Hsp) 25 和 II 类 MHC 抗原抗体对大鼠磨牙进行激光消融的牙髓反应的免疫细胞化学研究”细胞和组织研究 315・3。 -319 (2004)

Harada, H.他1名: "New perspectives on tooth development and dental stem cell niche"Archives of Histology and Cytology. (印刷中). (2004)

Harada, H. 等人：“牙齿发育和牙齿干细胞生态位的新观点”组织学和细胞学档案（2004 年）。

大島勇人(分担執筆): "パイロットアトラス 標本から学ぶ口腔の発生と組織"医歯薬出版株式会社. 209 (2003)

大岛隼人（撰稿人）：《Pilot Atlas：从标本中了解口腔的发育和组织》石药出版有限公司 209（2003）

Nakakura-Ohshima, K. 他3名: "Possible role of immunocompetent cells and the expression of heat shock protein-25 in the process of pulpal regeneration after tooth injury in rat molars"Journal of Electron Microscopy. 52・6. 581-591 (2003)

Nakakura-Ohshima, K. 等 3 人：“免疫活性细胞和热休克蛋白 25 的表达在大鼠磨牙牙髓再生过程中的可能作用”，电子显微镜杂志 52・6。 (2003)