Research on Dental Pulp Stem Cells and Pulpal Healing Process

牙髓干细胞与牙髓愈合过程的研究

• 批准号: 16390523
• 负责人: OHSHIMA Hayato
• 金额: $ 8.45万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390523/
• 关键词: adult stem cells; dental pulp; tooth replantation; tooth transplantation; odontoblasts; osteoblasts; BrdU; ROSA26; 半導体レーザー; 破骨細胞; ストレスタンパク質; 窩洞形成; 齧歯類; 再生; 発生

We investigated the cell dynamics in the process of tooth development and pulpal healing after tooth injuries and the adult stem cells in the dental tissue, and reached the following conclusion.1. Heat-shock protein (HSP)-25 protein was suggested to act as a switch between cell proliferation and terminal cyto-differentiation during odontogenesis.2. We proposed a new concept that the eternal tooth bud producing various dental progeny is formed at the apical end of continuously growing teeth, and a new term "apical bud" for indicating this specialized epithelial structure.3. We clarified pulpal responses to CrTmEr : YAG and CaAlAs laser irradiation. The GaAlAs laser may induce the formation of tertiary dentin by influencing the secretory activity of odontoblasts. However, higher energies may cause irreversible changes to the pulp, often leading to the formation of an intra-pulpal bone-like tissue.4. The appearance of TRAP-and CK-positive cells may be involved in the induction of bone tissue formation in dental pulp. Furthermore, the lack of proper oxygenated medium is quite decisive for the survival of odontoblast-lineage cells and that the occlusal force during and/or after the operation make the fate of these cells worse.5. Our experimental study using autogenic tooth transplantation into the sublingual region supported the notion that odontoblast-and osteoblast-lineage cells reside in the dental pulp.6. Aged pulp tissue still, possesses the defense capacity, and a variety of reactions could occur depending on the difference in the status of dentinal tubules and/or odontoblast processes in individuals.

我们研究了牙齿发育和牙损伤后牙髓愈合过程中的细胞动态以及牙组织中的成体干细胞，得出以下结论： 1．热休克蛋白(HSP)-25蛋白被认为在牙发生过程中充当细胞增殖和终末细胞分化之间的开关。 2．我们提出了产生各种牙后代的永恒牙芽是在不断生长的牙齿的根尖部形成的新概念，并提出了新术语“根尖芽”来表示这种特殊的上皮结构。 3．我们阐明了牙髓对 CrTmEr : YAG 和 CaAlAs 激光照射的反应。 GaAlAs激光可能通过影响成牙本质细胞的分泌活性来诱导三级牙本质的形成。然而，较高的能量可能会导致牙髓发生不可逆的变化，常常导致牙髓内骨样组织的形成。4． TRAP和CK阳性细胞的出现可能参与牙髓中骨组织形成的诱导。此外，缺乏适当的含氧介质对于成牙本质细胞谱系细胞的生存具有决定性作用，并且手术期间和/或手术后的咬合力使这些细胞的命运变得更糟。 5．我们使用自体牙齿移植到舌下区域的实验研究支持了成牙本质细胞和成骨细胞谱系细胞驻留在牙髓中的观点。6。老化的牙髓组织仍然具有防御能力，根据个体牙本质小管和/或成牙本质细胞过程状态的差异，可能会发生多种反应。

项目成果

Considerable subjects to understand repair responses of dental pulp after tooth injury from a biological point of view (in Japanese).

从生物学角度理解牙齿损伤后牙髓修复反应的相当多的课题（日语）。

• 发表时间: 2005
• 期刊: J Jpn Endodont Assoc 26(2)
• 作者: Ohshima;H.他5名;Ohshima H
• 通讯作者: Ohshima H

Cell dynamics in the process of pulpal healing following tooth injuries.

牙齿损伤后牙髓愈合过程中的细胞动力学。

• 发表时间: 2005
• 期刊: Journal of Hard Tissue Biology 14(2)
• 作者: Ohshima;H.
• 通讯作者: H.

The extended time to operate and the occlusal force influence determination of the pulpal healing pattern in replanted mouse molars.

延长的操作时间和咬合力影响再植小鼠磨牙牙髓愈合模式的确定。

• 发表时间: 2007
• 期刊: Cell and Tissue Research (印刷中)
• 作者: Hasegawa;T.他3名
• 通讯作者: T.他3名

Odontoblast responses to GaAlAs laser irradiation in rat molars: an experimental study using heat-shock protein-25 immunohistochemistry

• DOI: 10.1111/j.1600-0722.2006.00261.x
• 发表时间: 2006-02-01
• 期刊: EUROPEAN JOURNAL OF ORAL SCIENCES
• 影响因子: 1.9
• 作者: Tate, Y;Yoshiba, K;Ohshima, H
• 通讯作者: Ohshima, H

歯の損傷後の歯髄修復機構

牙齿损伤后的牙髓修复机制

• 发表时间: 2007
• 期刊: 歯科臨床研究 4・1
• 作者: Ikawa K;Nishioka T;Yu Z;Sugawara Y;Kawagoe J;Takizawa T;Primo V;Nikolic B;Kuroishi T;Sasano T;Shimauchi H;Takada H;Endo Y;Sugawara S;大島勇人
• 通讯作者: 大島勇人