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A histological investigation into the actual conditions of infected regions of apices of the teeth with apical periodontitis

根尖周炎牙齿根尖感染区实际情况的组织学调查

基本信息

批准号：
12671853
负责人：
MATSUO Takashi
金额：
$ 2.5万
依托单位：
The University of Tokushima
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2001
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12671853/
关键词：
apical periodontitis extra-radical area bacteria root dentin immunohistology SEM refractory apical periodontitis infected root canal treatment バイオフィルム感染根管免疫染色根尖孔

项目摘要

To investigate the apical region of the root with infected root canal is important not only for the elucidation of pathogenesis of apical periodontitis but for the development of treatments or diagnosis of it. Because apical regions of roots with infected canal could be the front of host and parasite. Hence, we examined immunohistologically the localization and prevalence of bacteria in the apical third of a root canal and the extra-radical area surrounding an apical foramen of the teeth with apical periodontitis. Moreover we examined these regions by scanning electron microscopy (SEM). In the apical portion of the infected root canal, 5. sanguis, P. nigrescens/P. intermedia, S. mutatis, L. plantarum, and L. case/ were frequently observed, and in the extra-radical regions, L. plantarum, S. mutans, P. nigrescens/P. intermedia were frequently observed. In particular, L. plantarum was most frequently detected in the extra-radical areas; indicating this bacteria would play important roles in the pathogenesis of apical periodontitis. These results showed the close relationship between extraradical and intra-canal bacteria in apical periodontitis. SEM observations showed that bacteria aggregated in the extra-radical area on a root surface and were related to bacteria of the intra-root canal, which was consistent with immunohistological observations described above. The aggregated bacteria in the extra-radical areas produced extra-cellular matrix and formed biofilm that prevented host-defending systems from eliminating parasites. These findings indicate that intra-canal bacteria in the apical portion invaded and affected extra-radical areas by forming biofilm, which caused the chronic lesion i.e. apical periodontitis.

研究感染根管的根尖区，不仅对根尖牙周炎发病机制的阐明，而且对根尖牙周炎的治疗和诊断具有重要意义。因为感染根管的根尖区域可能是寄主和寄生虫的前沿。因此，我们从免疫组织学上检查了根尖牙周炎患者根管根尖三分之一和根尖孔周围的根外区域细菌的定位和流行情况。此外，我们通过扫描电子显微镜（SEM）检查了这些区域。在感染根管的根尖部分，5。多血，P. nigrescens/P。在超根尖区，常见的有L. plantarum、S. mutans、P. nigrescens/P.；中间媒介经常被观察到。其中植物乳杆菌最常见于超根区；提示该细菌在根尖牙周炎发病中起重要作用。结果表明，根尖牙周炎的根外菌群与根管内菌群的关系密切。扫描电镜观察显示，细菌聚集在根表面的超根区，与根管内的细菌有关，这与上述免疫组织学观察结果一致。聚集在超自由基区域的细菌产生细胞外基质并形成生物膜，阻止宿主防御系统清除寄生虫。提示根管内细菌侵入根外区，形成生物膜，引起根尖牙周炎。