Development of the new diagnostic methods and treatments of dental caries based upon caliology

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We are trying to develop the new diagnostic methods and treatments of dental caries on the basis of the concept that dental caries is infection disease caused by oral microorganisms and longevity of tooth lifetime will be prolonged by the conservation of tooth material during the caries treatment. Specifically, we studied on the bacteria in caries lesions, caries activity, detection and removal of caries lesions, clinical caries risks, and epidemiological surveys on caries. In consequence, we identified the species of bacteria using 16S rDNA extracted from resin-embedded caries dentin, and developed diagnosis procedures using objective color, autofluorescence, or pH-imaging of caries lesions. We elucidated the effects of chloramines and sodium hypochlorite on carious dentin, and apoptosis of pulpal cells by mechanical and chemical stresses on cavity preparation. We evaluated the clinical caries risks in terms of resin composite restoration or of a habitual chewing site. We also clarified the proportion of bacteria in bioflim formed on restorative materials and effects of PMTC on the simplified eradication system for oral bacteria using chlorhexidine.