Inhibitory Effects of Ipriflavone on Resorption of Augmented Bone

依普沙芬(IP)是一种治疗骨质疏松症的药物，在骨重建过程中可能会促进骨形成。本研究的目的是通过引导骨再生(GBR)程序，观察IP对增强骨(矿化骨)吸收的抑制作用。成年雄性日本大耳白兔15只，暴露颅骨，在全身麻醉下将标准半球形钛帽(高4 mm，直径8 mm)置入颅骨内。用1%羟丙基纤维素(HP)溶液制备10 mg/ml的IP。动物分为3组：HP组，不摄入IP；IP后组，GBR后每日IP(10 mg/kg)组和IP前组；一个盖子在3个月愈合后被摘除。愈合4个月后，处死动物，进行组织学检查，计算每一切片中由矿化骨和骨髓间隙组成的新生组织的面积百分比。与所有组的对照部位相比，测试部位的新生率都有所降低。在HP组中，我们观察到对照部位和测试部位之间的显著差异(93.8%±4.6%比73.9%±3.7%，P&lt；0.05)。然而，IP前和IP后两组之间的差异无统计学意义。对照组的骨矿化量分别为28.9%±2.9%、25.7%±1.8%、36.2%±0.9%，差异有统计学意义(P&lt；0.05)。而ALL组试验区与对照组和试验区之间无显著差异。我们认为IP能抑制去钛帽后新生组织的吸收，促进GBR术后矿化骨的形成。

Ipriflavone (IP), a drug used in the treatment of osteoporosis, may promote bone formation during bone remodeling. The purpose of this study was to investigate the inhibitory effects of IP on resorption of augmented bone (mineralized bone) using a guided bone regeneration (GBR) procedure. In 15 adult male white Japanese rabbits the calvarial bone was exposed and a standardized hemispherical titanium cap (4 mm in height, 8 mm in diameter) was then placed into the bone under general anesthesia. IP was prepared for 10 mg/ml using 1% hydroxypropyl cellulose (HP) solution. The animals were divided into 3 groups : HP group, no intake of IP ; Post-IP group, daily intake of IP (10 mg/kg) after GBR and Pre-IP group ; daily intake of IP (10 mg/kg) throughout the study. One cap was removed after 3-month healing. After 4 months of healing, the animals were euthanized and examined histologically.The percentage area of newly generated tissue consisting of mineralized bone and marrow spaces in each section was calculated. The rate of the newly generated tissue in the test sites was decreased when compare to the control sites in all groups. In the HP groups, we observed a significant difference between the control and test sites (93.8% ± 4.6% versus 73.9% ± 3.7%, P < 0.05). However, there was no statistically significant difference between the Pre-IP and Post-IP groups. Also, there was a statistically significant difference among the groups in the amount of mineralized bone in the control sites (28.9% ± 2.9% versus 25.7% ± 1.8% versus 36.2% ± 0.9%, P < 0.05). However, there was no significant difference between the test sites, and the control and test sites in ail groups.We concluded that IP will inhibit resorption of newly generated tissue after removal of titanium cap and promote mineralized bone formation after GBR procedure.