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Ecological method to control methicillin resistant Staphylococcus aureus

防治耐甲氧西林金黄色葡萄球菌的生态方法

基本信息

批准号：
12672280
负责人：
KAKINOHANA Shige
金额：
$ 2.11万
依托单位：
University of the Ryukyus
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2000
资助国家：
日本
起止时间：
2000 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12672280/
关键词：
Staphylococcus aureus nosocomial infection carrier drug resistant

项目摘要

Methicillin resistant Staphylococcus aureus (MRSA) as a causative agent of nosocomial infection has been notorious in the past 2 decades, and now control the pathogen in the hospital is a pressing problem in the world. The present study was planned to find a method to control MRSA not depending on anti-microbial chemotherapy but depend on an ecological method using some environmental organisms, and also the study was aimed at cultivating nurse specialists for infection control. Examinations of nasal vestibulum for the nurses working at the hospital revealed that about 40% of the nurses carried S. aureus and about 25% of the carriers had MRSA. In three times examinations every 2 months, it was clarified that there were constant carriers, constant non-carriers, and intermittent carriers.Environmental organisms were collected and each organisms was examined for the inhibition of MRSA. Some organisms inhibited the growth of MRSA on an agar plate and in mixed culture in broth. One strain i-1 markedly inhibited the growth of MRSA. The inhibitory activity was examined on the mouse skin from which the hair was removed. The skin was colonized by MRSA for 10 to 14 days after inoculation but GPR strain i-1 disappeared within a few days. In 2 groups of mice, one inoculated MRSA, another inoculated MRSA and i-1, the period of colonization by MRSA was almost the same. GPR i-1 strain produced MRSA inhibitor in the culture supernatant. The inhibitor was concentrated, applied on the mouse skin, and the MRSA was inoculated. However, no inhibitory effect was found. In conclusion, we found many strains of environmental Gram positive rods which inhibited the growth of MRSA in vitro. However, it was not active on the animal skin surface. The inhibitor should be purified to investigate whether it can be used as anti-microbial drug or not.

耐甲氧西林金黄色葡萄球菌（MRSA）作为医院感染的病原体，在过去20年里一直臭名昭著，控制医院内的病原菌已成为世界范围内的紧迫问题。本研究计划寻找一种不依赖于抗微生物化疗而是依赖于使用一些环境生物的生态方法来控制MRSA的方法，并且该研究的目的是培养感染控制的护理专家。对在该医院工作的护士进行鼻前庭检查发现，约40%的护士携带金黄色葡萄球菌，约25%的携带者携带MRSA。每2个月进行3次检查，明确持续携带者、持续非携带者和间歇性携带者。收集环境微生物，检测每种微生物对MRSA的抑制作用。一些微生物抑制琼脂平板上和肉汤混合培养物中 MRSA 的生长。一种菌株 i-1 显着抑制 MRSA 的生长。在去除毛发的小鼠皮肤上检查抑制活性。接种后 10 至 14 天，皮肤上 MRSA 定植，但 GPR 菌株 i-1 在几天内消失。在两组小鼠中，一组接种MRSA，另一组接种MRSA和i-1，MRSA的定植期几乎相同。 GPR i-1 菌株在培养物上清液中产生 MRSA 抑制剂。将抑制剂浓缩后涂在小鼠皮肤上，接种MRSA。然而，没有发现抑制作用。总之，我们在体外发现了许多抑制MRSA生长的环境革兰氏阳性杆菌菌株。然而，它在动物皮肤表面没有活性。抑制剂需要纯化以研究其是否可以用作抗微生物药物。