The role of the poly(A)-specific ribonuclease (PARN) in mRNA decay

聚腺苷酸特异性核糖核酸酶 (PARN) 在 mRNA 降解中的作用

• 批准号: 5311538
• 负责人: Professor Dr. Elmar Wahle
• 金额: --
• 依托单位: Abteilung Allgemeine Biochemie
• 依托单位国家: 德国
• 项目类别: Research Units
• 财政年份: 2001
• 资助国家: 德国
• 起止时间: 2000-12-31 至 2004-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/5311538?language=en
• 关键词: role; poly; specific; ribonuclease; PARN

Degradation of the poly(A) tail (deadenylation) is the first and rate-limiting step in the decay of many mRNAs. Deadenylation is also used as a means of translational control during oocyte maturation and early development. We have purified and cloned a poly(A)-specific 3'-exoribonuclease (PARN) that is responsible for deadenylation in oocytes and possible also in somatic cells. Surprisingly, the nuclease prefers substrate RNAs carrying a 5' cap. The current application has three goals: First, overproduction of sufficient quantities of PARN in a eukaryotic system to allow a characterisation of the enzyme's functional properties, in particular cap dependence and processivity; second, the investigation of the relationship between mRNA translation and decay; and, third, the generation of a knock-out mouse to obtain definitive evidence regarding the physiological role of PARN.

poly(A)尾部的降解（deadenylation）是许多mrna衰变的第一步和限速步骤。在卵母细胞成熟和早期发育过程中，死蛋白化也被用作翻译控制的一种手段。我们纯化并克隆了一种聚(a)特异性的3'-外核糖核酸酶（PARN），该酶在卵母细胞和体细胞中可能负责死基化。令人惊讶的是，核酸酶更喜欢携带5'帽的底物rna。目前的应用有三个目标：第一，在真核系统中过量生产足够数量的PARN，以允许表征酶的功能特性，特别是帽依赖性和加工性；二是mRNA翻译与衰变关系的研究；第三，基因敲除小鼠的产生，以获得关于PARN生理作用的明确证据。