Ghost tangle elimination mechanism in the CA1 of parkinsonism-dementia complex of Guam

用免疫组织化学和免疫电子显微镜观察了帕金森病痴呆复合体CA1区鬼影缠结的消除机制。神经元中的神经原纤维缠结主要由粗大的螺旋细丝(PHF)组成，而鬼影缠结主要由直管(STS)组成。免疫组织化学显示星形细胞突起与幽灵缠结紧密交织。免疫电子显微镜下，星形细胞胶质细丝与幽灵缠结的STS混杂在一起。然而，小胶质细胞的参与并不显著。在溶酶体中没有观察到神经原纤维缠结，包括PHF和STS。结果表明，幽灵缠结被星形胶质细胞消除。然而，由于死后自溶的改变，星形胶质细胞的质膜在这些观察中并不清楚。因此，消除星形胶质细胞内或细胞外空间的实际位置并不清楚。为了克服这一问题，使用动物和NFT脑内接种进行实验研究是不可避免的。

The elimination mechanism of ghost tangle was observed immunohistochemically and immunoelectron microscopically in the CA1 of the parkinsonism-dementia complex of Guam.Neurofibrillary tangles in the neurons were mainly composed of pared helical filaments (PHFs), but the ghost tangles were of straight tubules (STs). Astrocytic processes were tightly intertwined with ghost tangles in immunohistochemistry. Immunoelectronmicroscopically, astrocytic glial filaments were admixed with the ghost tangle STs. However, microglial participation was not remarkable. Neurofibrillary tangles, including PHFs and STs, were never observed in the lysosomes.The findings indicate that the ghost tangles were eliminated by the astrocytes. However, the plasma membrane of the astrocytes were not clear in these observations due to postmortem autolytic alteration. Thus the practical sites, that is, inside of astrocytes or in the extracellular space, were not clear in ghost tangle elimination.To overcome the problem, experimental study is inevitable using animals and NFT inoculation to the brains.