DEVELOPMENT OF BIOCHEMICAL IC CHIPS

生化IC芯片开发

• 批准号: 13305017
• 负责人: IKUTA Koji
• 金额: $ 36.03万
• 依托单位: NAGOYA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (A)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13305017/
• 关键词: Biochemical IC; Micro Stereolithography; Photo Curable Polymer; Biocompatible Materials; Tailor-made Medicine; Implantable Micro Device; Micro Chemical Analysis System; Optical Drive; テーラーメイド製薬

Biochemical IC chips for future micro/nano chemical device have been investigated. We took a systematic research approach for development compared with conventional research project.1) New fabrication process to create real three dimensional micro/nano structures has been developed. The micro/nano stereo lithography process originally developed in Ikuta's laboratory was further advanced for this project. Nano scale needle and tweezers were fabricated and driven by laser trapping. Smooth remote drive and ft Newton scale force sensing were demonstrated experimentally.2) New chip-sets of biochemical IC chips have been successfully developed and their functions were verified. Cell-free protein synthesis with advanced micro pump chips was succeeded. Several proteins have been synthesized from DNA without any living cell. This function is the key technology for not only post genome research but also order-made medicine based.3) Homogenizer chips to destroy cell and tissue in micro scale without damaging cell protein. Simple and effective coupling method between biochemical IC chips was developed. The silicon-rubber coupling can make a tight sealing. These results directly contribute to real total micro analysis process and devices.4) Artificial kidney device utilizing micro stereo lithography has been developed. Miniaturization and rat test were carried out successfully in Prof. Nagakura's lab.5) New technique for cell biology by using micro fluidics has been proposed and verified by Prof. Sokabe and Prof. Naruse. Since many new knowledge in life science were obtained through the new approach, it was made clear that micro chemical devices such as biochemical IC chips can contribute to basic life science as well as biomedical applications.

面向未来微纳化学器件的生化IC芯片已被研究。与传统的研究项目相比，我们采取了系统的研究方法进行开发。1)开发了制造真实三维微/纳米结构的新工艺。最初由Ikuta实验室开发的微纳立体光刻工艺为这一项目进一步推进。制作了纳米级的针和镊子，并用激光捕获驱动。2)研制了新型生化IC芯片组，并对其功能进行了验证。利用先进的微泵芯片实现了无细胞蛋白质合成。在没有任何活细胞的情况下，已经从DNA合成了几种蛋白质。这一功能不仅是后基因组研究的关键技术，也是定制化医学基础的关键技术。3)均质机芯片可以在不破坏细胞蛋白质的情况下，在微观范围内破坏细胞和组织。提出了一种简单有效的生化IC芯片间的耦合方法。硅橡胶联轴器可实现紧密密封。这些结果直接为真正的全显微分析过程和设备做出了贡献。4)研制了基于微立体光刻的人工肾装置。在Nagakura教授的实验室成功地进行了小型化和大鼠实验。5)Sokabe教授和Naruse教授提出并验证了利用微流控技术进行细胞生物学的新技术。由于通过这种新方法获得了许多生命科学方面的新知识，因此可以清楚地看到，生化IC芯片等微型化学设备可以为基础生命科学和生物医学应用做出贡献。

项目成果

K. Ikuta, K. Ikeda, A. Takahashi, S. Maruo: "Artificial cellular device with cell-free protein synthesis ability constructed by chemical IC chips"Proc. of the IEEE Conference on Nanotechnology (IEEE NANO 2001). 134-139 (2001)

K. Ikuta，K. Ikeda，A. Takahashi，S. Maruo：“由化学IC芯片构建的具有无细胞蛋白质合成能力的人工细胞装置”Proc。

Wang JG, Miyazu M, Matsushita E, Sokabe M, Naruse K: "Uniaxial cyclic stretch induces focal adhesion kinase (FAK) tyrosine phosphorylation followed by mitogen-activated protein kinase (MAPK) activation"Biochem Biophys Res Commun. 288. 356-361 (2001)

Wang JG、Miyazu M、Matsushita E、Sokabe M、Naruse K：“单轴循环拉伸诱导粘着斑激酶 (FAK) 酪氨酸磷酸化，随后激活丝裂原激活蛋白激酶 (MAPK)”Biochem Biophys Res Commun。

S.Maruo, K.Ikuta, H.Korogi: "Submicron manipulation tools driven by light in a liquid"Applied Physics Letters. 82-1. 133-135 (2003)

S.Maruo、K.Ikuta、H.Korogi：“液体中光驱动的亚微米操纵工具”应用物理快报。

S.Maruo, K.Ikuta, H.Korogi: "Optically driven micromanipulation tools fabricated by two-photon microstereolithography"Mat. Res. Soc. Symp. Proc.. 739(In press). (2003)

S.Maruo、K.Ikuta、H.Korogi：“通过双光子微立体光刻技术制造的光学驱动微操作工具”Mat。

生田幸士: "化学IC入門"マテリアルステージ. 1・10. 5-9 (2002)

生田浩二：《化学 IC 概论》材料阶段 1・10（2002 年）。