Development of biodegradable matrix, which promotes axonal regenerations

开发可生物降解的基质，促进轴突再生

基本信息

批准号：
13357014
负责人：
SUZUKI Yoshihisa
金额：
$ 32.36万
依托单位：
KYOTO UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2003
项目状态：
已结题

项目摘要

Freeze-dried alginate sponge cross-linked with covalent bonds has been demonstrated to enhance nerve regeneration in peripheral nerves and spinal cord. The present study examined at early stages after surgery the outgrowth of regenerating axons and reactions of astrocytes at the stump of transected spinal cord in young rats. Two segments (Th7-8) were resected, and alginate was implanted in the lesion. As controls, collagen gel was implanted in place of alginate or the lesion was left without implantation. Two and 4 weeks after surgery, nerve outgrowth and astrocyte reactions were examined. Many regenerating axons, some of which were accompanied by astrocytic processes, were found to extend from the stump into the alginate-implanted lesion. In the all non-implanted animals, large cystic cavities were formed at both interfaces with no definite axonal outgrowth into the lesion. In collagen-implanted animals, cavity formation was found in some rats, and regenerating axons once formed at the stumps did not extend further into the lesion. Astrocytic processes extending into alginate-implanted lesion had no basal laminae, whereas those found in control experiments were covered by basal laminae. These findings suggest that alginate contributed to reducing the barrier composed of connective tissues and reactive astrocytic processes, and served as a scaffold for the outgrowth of regenerating axons and elongation of astrocytic processes.

已证明与共价键交联的冷冻干燥藻酸盐海绵可以增强周围神经和脊髓的神经再生。本研究在手术后的早期检查了轴突再生轴突的生长和星形胶质细胞在年轻大鼠中跨性脊髓树桩上的反应。切除了两个片段（TH7-8），并将藻酸盐植入病变中。作为对照，植入胶原蛋白凝胶代替藻酸盐，或者没有植入病变。手术后两周和4周，检查了神经产物和星形胶质细胞反应。发现许多再生轴突，其中一些伴随着星形胶质细胞过程，发现从树桩延伸到藻酸盐植入的病变。在所有非植入动物中，在两个界面上都形成了大囊性腔，没有明确的轴突生长。在胶原蛋白植入的动物中，在一些大鼠中发现了腔形成，曾经在树桩上形成的再生轴突并未进一步延伸到病变中。延伸到藻酸盐植入病变的星形胶质细胞过程没有基底层薄片，而在对照实验中发现的病变则由基底层层覆盖。这些发现表明，藻酸盐有助于减少由结缔组织和反应性星形胶质细胞过程组成的屏障，并作为再生轴突生长和星形胶质细胞过程伸长的脚手架。