权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Development of new gel used for artificial nerve to differenciate neural stem cells and to promote elongation ofaxons in damaged peripheral and central nervous system

开发用于人工神经的新型凝胶，以分化神经干细胞并促进受损外周和中枢神经系统的轴突伸长

基本信息

批准号：
14207073
负责人：
SUZUKI Yoshihisa
金额：
$ 32.28万
依托单位：
Kyoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (A)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Transplantation of neural stem cells has been studied extensively for the treatment of central nervous system(CNS) injury (Seaberg and van-der-Kooy,2002;Van-der-Kooy and Weiss, 2000;Fuchs and Segre,2000). There have been various approaches to transplantation, including direct local administration by injection with or without artificial substrates as cell carriers (Wu et al.,2001) into the injured brain (Fricker et al.,1999;Gage et al.,1995) and spinal cord (Akiyama et al.,2001). On the other hand, gene therapeutic agents (Bajocchi et al.,1993), neurotrophic factors (Araujo and Hilt,1997) and drugs such as nitecapone (Mannisto et al.,1992) have been administered through the CSF.We examined the distribution of hippocampus-derived neural stem cells on the spinal cord surface for up to 3 weeks following injection through the 4th ventricle. The injected cells were disseminated as tiny spots on the pia mater of the spinal cord and proliferated into large cell-clusters. On both the dorsal and … More ventral side, cell clusters increased in number rapidly up to 5 days after injection, and thereafter decreased gradually due to the coalition of neighbouring clusters. Concomitantly, individual cell clusters continuously increased in size, occupying almost 50% of the spinal cord surface. Cell attachment was usually found around blood vessels, along which cells invaded into the spinal cord. In the injured site, cells migrated into the lesion and were integrated into the spinal cord tissue, some of which had differentiated into astrocytes 1-2 weeks after injection. BrdU-uptake experiments demonstrated that the transplanted cells proliferated within the host cerebrospinal fruid.These results indicate that application of neural stem cells through the ventricle is a unique method to disseminate cells all over the spinal cord, and that they can migrate and be integrated into the injured spinal cord.Transplantation of neural stem cells has been studied extensively for the treatment of central nervous system(CNS) injury (Seaberg and van-der-Kooy,2002;Van-der-Kooy and Weiss, 2000;Fuchs and Segre,2000). There have been various approaches to transplantation, including direct local administration by injection with or without artificial substrates as cell carriers (Wu et al.,2001) into the injured brain (Fricker et al.,1999;Gage et al.,1995) and spinal cord (Akiyama et al.,2001). On the other hand, gene therapeutic agents (Bajocchi et al.,1993), neurotrophic factors (Araujo and Hilt,1997) and drugs such as nitecapone (Mannisto et al.,1992) have been administered through the CSF. Less

神经干细胞移植治疗中枢神经系统（CNS）损伤已被广泛研究（seabberg and van-der-Kooy,2002; van-der-Kooy and Weiss, 2000;Fuchs and Segre,2000）。有多种移植方法，包括直接局部注射有或没有人工基质作为细胞载体（Wu et al.,2001）到受伤的大脑（Fricker et al.,1999;Gage et al.,1995）和脊髓（Akiyama et al.,2001）。另一方面，基因治疗剂（Bajocchi et al.,1993）、神经营养因子（Araujo and Hilt,1997）和尼替卡酮（Mannisto et al.,1992）等药物已通过脑脊液给药。通过第四脑室注射后，我们检测了海马体来源的神经干细胞在脊髓表面的分布长达3周。注射后的细胞以小点分布于脊髓硬膜上，并增殖成大细胞团。注射后5天，在背侧和更腹侧，细胞簇数量迅速增加，随后由于邻近细胞簇的联合而逐渐减少。与此同时，单个细胞簇的大小不断增加，几乎占据脊髓表面的50%。通常在血管周围发现细胞附着，细胞沿着血管侵入脊髓。在损伤部位，细胞迁移到病变部位并融入脊髓组织，部分细胞在注射后1-2周分化为星形胶质细胞。brdu摄取实验表明，移植细胞在宿主脑脊液中增殖。这些结果表明，通过脑室应用神经干细胞是一种独特的方法，使细胞遍布脊髓，并且它们可以迁移和整合到损伤的脊髓中。神经干细胞移植治疗中枢神经系统（CNS）损伤已被广泛研究（seabberg and van-der-Kooy,2002; van-der-Kooy and Weiss, 2000;Fuchs and Segre,2000）。有多种移植方法，包括直接局部注射有或没有人工基质作为细胞载体（Wu et al.,2001）到受伤的大脑（Fricker et al.,1999;Gage et al.,1995）和脊髓（Akiyama et al.,2001）。另一方面，基因治疗剂（Bajocchi et al.,1993）、神经营养因子（Araujo and Hilt,1997）和尼替卡酮（Mannisto et al.,1992）等药物已通过脑脊液给药。少