Colloidal crystallization of protein molecules
Colloidal crystallization of protein molecules
批准号:
24656016
负责人:
SUZUKI Yoshihisa
金额:
$2.58万
依托单位国家:
日本
项目类别:
Grant-in-Aid for Challenging Exploratory Research
财政年份:
2012
资助国家:
日本
项目状态:
已结题
起止时间:
2012-04-01 至 2014-03-31
中文摘要
我们尝试用胶体结晶法而不是传统的盐析法来结晶蛋白质分子。用超纯水或稀盐酸溶液离心透析浓缩蛋白质分子,但不能得到典型的蛋白质胶体晶体。此外,我们还证实了通过液-液相分离的新结晶过程。
英文摘要
We tried to crystalliza protein molecules not via conventional salting-out method but via colloidal crystallization method. Protein molecules were concentrated by centrifugal dialyzattion against ultra-pure water or dilute aqueous HCl solutions.Unfortunately, we could not obtain typical colloidal crystals of protein. Instead, normal protein crystals without using conventional salting-out method.In addition, we also confirm novel crystallization processes via liquid-liquid phase separations.
期刊论文(43)
专著(0)
科研奖励(0)
会议论文
登录
查看更多内容
Growth rate measurements of lysozyme crystals under microgravity conditions by laser interferometry.
DOI:
10.1063/1.4826090
发表时间:
2013-10
期刊:
The Review of scientific instruments
影响因子:
--
作者:
[I. Yoshizaki;K. Tsukamoto;T. Yamazaki;K. Murayama;Kentaro Oshi;S. Fukuyama;T. Shimaoka;Yoshihisa Suzuki;M. Tachibana]
通讯作者:
I. Yoshizaki;K. Tsukamoto;T. Yamazaki;K. Murayama;Kentaro Oshi;S. Fukuyama;T. Shimaoka;Yoshihisa Suzuki;M. Tachibana
撥水性基板を用いたコロイド結晶のグレインサイズ制御
使用防水基材控制胶体晶体的晶粒尺寸
DOI:
--
发表时间:
2012
期刊:
影响因子:
--
作者:
[伊藤大祐, 鈴木良尚]
通讯作者:
鈴木良尚
リゾチーム存在下でのグルコースイソメラーゼ結晶の平衡温度
溶菌酶存在下葡萄糖异构酶晶体的平衡温度
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[藤原貴久, 鈴木良尚, 佐藤正英, 佐崎元, 勝野弘康]
通讯作者:
勝野弘康
Growth Mechanism of Lysozyme Crystals in The International Space Station Based on The Analysis of In-Situ Interferometric Observation
基于原位干涉观测分析国际空间站溶菌酶晶体的生长机制
DOI:
--
发表时间:
2013
期刊:
影响因子:
--
作者:
[Katsuo Tsukamoto, Izumi Yoshizaki, Yoshihisa Suzuki, Hitoshi Miura, Gen Sazaki, Masaru Tachibana, Kentaro Oshi and Manuel Juan Garcia-Ruiz]
通讯作者:
Kentaro Oshi and Manuel Juan Garcia-Ruiz
DOI:
--
发表时间:
2013
期刊:
Natural Science
影响因子:
--
作者:
[Masahide Sato, Hiroyasu Katsuno and Yoshihisa Suzuki, Atsushi Mori and Yoshihisa Suzuki, Atsushi Mori and Yoshihisa Suzuki]
通讯作者:
Atsushi Mori and Yoshihisa Suzuki
共 32 条
Crystallization and crystallography of proteins at in vivo salt concentrations
-
批准号:18K04960
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.83万
-
财政年份:2018
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Development of treatment for injury of central and peripheral nervous system
-
批准号:22390333
-
项目类别:Grant-in-Aid for Scientific Research (B)
-
资助金额:$12.23万
-
财政年份:2010
-
负责人:SUZUKI Yoshihisa
-
依托单位:
An Examination on Market Efficiency with Behavioral Characteristics Across Investor Categories
-
批准号:20530274
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.41万
-
财政年份:2008
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Studies on the activation volume of an incorporation process by in situ measurements of step velocities under high pressure
-
批准号:19760009
-
项目类别:Grant-in-Aid for Young Scientists (B)
-
资助金额:$2.39万
-
财政年份:2007
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Treatment of central and/or peripheral nerve injury using artificial matrix and/or differentiated bone marrow stromal cell
-
批准号:17209056
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$31.95万
-
财政年份:2005
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Development of new gel used for artificial nerve to differenciate neural stem cells and to promote elongation ofaxons in damaged peripheral and central nervous system
-
批准号:14207073
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$32.28万
-
财政年份:2002
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Artificial Market Control on the Security Markets
-
批准号:14530110
-
项目类别:Grant-in-Aid for Scientific Research (C)
-
资助金额:$2.11万
-
财政年份:2002
-
负责人:SUZUKI Yoshihisa
-
依托单位:
Development of biodegradable matrix, which promotes axonal regenerations
-
批准号:13357014
-
项目类别:Grant-in-Aid for Scientific Research (A)
-
资助金额:$32.36万
-
财政年份:2001
-
负责人:SUZUKI Yoshihisa
-
依托单位:
海外基金