Biodesulfurization using thermophilic bacteria and identification of bds genes
嗜热菌生物脱硫及bds基因鉴定
基本信息
- 批准号:13650859
- 负责人:
- 金额:$ 2.3万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2003
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Recalcitrant organosulfur compounds such as dibenzothiophene (DBT) derivatives in light gas oil (LGO) cannot be removed by hydrodesulfurization using metallic catalysts. The thermophilic bacteria, Bacillus subtilis WU-S2B and Mycobacterium phlei WU-F1, were isolated for its ability to grow on DBT as the sole source of sulfur at 50℃. In addition to DBT, WU-S2B and WU-F1 also could desulfurize alkylated DBTs such as 4, 6-dimethyl DBT through a sulfur-specific degradation pathway with the selective cleavage of carbon-sulfur bonds. Moreover, when resting cells of WU-F1 were incubated at 45℃ with two types of hydrodesulfurized LGOs in the reaction mixtures containing 50%(v/v)oils, the biodesulfurization reduced the sulfur contents from 120 to 50 ppm S (F-LGO) and from 34 to 15 ppm S (X-LGO), respectively. Gas chromatography analysis with an atomic emission detector revealed that the peaks of alkylated DBTs including 4-methyl DBT, 4, 6-dimethyl DBT, and 3, 4, 6-trimethyl DBT significantly decreased after the biodesulfurization. The DBT-desulfurization genes, bdsABC, were cloned from these two bacteria, and the flavin reductase genes, frb and frm, were also cloned from WU-S2B and Wu-F1, respectively. The recombinant WU-F1 carrying one more set of bdsABC and frm was constructed, and the desulfurizing activity of the recombinant strain was 2. 2-fold higher than that of the wild type strain WU-F1.
轻气油(LGO)中的顽固性有机硫化合物如二苯并噻吩(DBT)衍生物不能通过金属催化剂加氢脱硫去除。在50℃条件下,嗜热细菌枯草芽孢杆菌WU-S2B和真菌分枝杆菌WU-F1能够在单硫源DBT上生长。除DBT外,WU-S2B和WU-F1还可以通过选择性裂解碳硫键的硫特异性降解途径脱硫烷基化DBT,如4,6 -二甲基DBT。另外,在含50%(v/v)油的反应混合物中,将WU-F1的静息细胞与两种加氢脱硫的lgo在45℃下培养,生物脱硫的硫含量分别从120 ppm S (F-LGO)降至50 ppm S (X-LGO),从34 ppm S降至15 ppm S (X-LGO)。原子发射检测器气相色谱分析表明,4-甲基DBT、4,6 -二甲基DBT和3,4,6 -三甲基DBT的烷基化峰在生物脱硫后显著降低。从这两种细菌中克隆出了dbt脱硫基因bdsABC,从WU-S2B和Wu-F1中分别克隆出了黄素还原酶基因frb和from。重组菌株WU-F1多携带一组bdsABC和frm,重组菌株的脱硫活性为2。比野生型菌株WU-F1高2倍。
项目成果
期刊论文数量(17)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Kohtaro Kirimura et al.: "Identification and functional analysis of the genes encoding dibenzothiophene-desulfurizing enzymes from thermophilic bacteria"Appl.Microbiol.Biotechnol.. (In press). (2004)
Kohtaro Kirimura 等人:“来自嗜热细菌的编码二苯并噻吩脱硫酶的基因的鉴定和功能分析”Appl.Microbiol.Biotechnol..(正在出版)。
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- 影响因子:0
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- 通讯作者:
Kohtaro Kirimura, et al.: "Thermophilic biodesulfurization of hydrodesulfurized light gas oils by Mycobacterium Phlei WU-F1"FEMS Microbiol.Lett.. 221. 137-142 (2003)
Kohtaro Kirimura 等:“通过 Mycobacter Phlei WU-F1 对加氢脱硫轻质瓦斯油进行嗜热生物脱硫”FEMS Microbiol.Lett.. 221. 137-142 (2003)
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- 影响因子:0
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Kohtaro Kirimura, et al.: "Thermophilic biosedulfurization of naphthothiophene and 2-ethylnaphthothiophene by a dibenzothiophene-desulfurizing bacterium, Mycobacterium phlei WU-F1"Appl.Microbiol.Biotechnol.. 58. 237-240 (2002)
Kohtaro Kirimura 等人:“二苯并噻吩脱硫细菌,草分枝杆菌 WU-F1 对萘并噻吩和 2-乙基萘并噻吩的高温生物硫化”Appl.Microbiol.Biotechnol.. 58. 237-240 (2002)
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- 发表时间:
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- 影响因子:0
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- 通讯作者:
Kohtaro Kirimura, et al.: "Thermophilic biodesulfurization of dibenzothiophene and its derivatives by Mycobacterium phlei WU-F1"FEMS Microbiol.Lett.. 204. 129-133 (2001)
Kohtaro Kirimura 等:“草分枝杆菌 WU-F1 对二苯并噻吩及其衍生物的嗜热生物脱硫”FEMS Microbiol.Lett.. 204. 129-133 (2001)
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- 影响因子:0
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Kohtaro Kirimura et al.: "Cloning of a gene encoding flavin reductase coupling with dibenzothiophene monooxygenase through coexpression screening using indigo production as selective indication"Biochem. Biophys. Res. Commun.. 313. 570-575 (2004)
Kohtaro Kirimura 等人:“通过使用靛蓝生产作为选择性指示的共表达筛选,克隆编码黄素还原酶与二苯并噻吩单加氧酶偶联的基因”Biochem。
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KIRIMURA Kohtaro其他文献
KIRIMURA Kohtaro的其他文献
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Generation of the cell factories for bio-based organic acid production through improvement of filamentous fungi by metabolic engineering
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- 批准号:
22580093 - 财政年份:2010
- 资助金额:
$ 2.3万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
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