Screening of the naturally nematicidal compounds against plant parasitic nematode Pratylenchus penetrans Mechanisms

天然杀线虫化合物对植物寄生线虫穿透短体线虫的筛选及其机制

• 批准号: 13660107
• 负责人: KIMURA Yasuo
• 金额: $ 2.24万
• 依托单位: Tottori University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13660107/
• 关键词: nematicide; nematode; plant parasite; natural compound; fungus; 植物寄生性線虫; α、β-dehydrocurvularin; terrestric acid; 植物毒; 微生物; Aspergillus sp.

In the course of a screening search for new nematicidal compounds among the fungal metabolites, using bioassay method with a root-lesion nematode Pratylenchus penetrans, suitable for developing new nematicides and new lead compounds, I found the presence of active compounds in the metabolites of Penicillium bilaiae Chalabuda, Penicillium cf. simplicissimum, and an unidentified Aspergillus sp.Biosassay-guided fractionation led to the isolation of four active compounds,2,6-pyridinedicauboxylic acid,6-methoxypicolinic acid, p-hydroxyacetophenone and a new compound named pratynolene from the culture filtraste of Penicillium bilaiae Chalabuda. Three active compounds together with a related but inactive compound were also isolated from the mycelial mats of Penicillium cf.simplicissimum. Four curvuralin analogs were obtained from the culture filtrate and mycelial mats,although two of them were inactive.From these results together with bioassays with rice and lettuce seedlings,the following facts became clear.1) Active compounds are present in the culture filtrate or mycelial mats.2) Some of the nematicidal compounds shows phytotoxic activity.3) Some compounds have selectivity such as a compound shows nematicidal activity against P.penatrans but sit is inactive against C. elegance.

在真菌代谢产物中筛选新的杀线虫化合物的过程中，使用适合于开发新的杀线虫剂和新的先导化合物的根病变线虫Pratylenchus cystrans的生物测定方法，我发现在Penicillium bilaiae Chalabuda、Penicillium cf.利用生物活性测定法从毕莱青霉（Penicillium bilaiae Chalabuda）的培养滤液中分离得到4个活性化合物：2，6-吡啶二羧酸、6-甲氧基吡啶甲酸、对羟基苯乙酮和一个新化合物pratynolene。三个活性化合物连同一个相关的，但无活性的化合物也被分离出的菌丝垫的青霉CF。simplicissimum。从培养滤液和菌丝垫中获得了四种弯孢菌素类似物，尽管其中两种是无活性的。以下事实变得清楚：1）活性化合物存在于培养滤液或菌丝垫中。2）一些杀线虫化合物显示植物毒性活性。3）一些化合物具有选择性，例如化合物显示出对P.penatrans的杀线虫活性，但对C.优雅

项目成果

Kimura,Y., Kusano,M., Nakahara,S.: "Nematicidal compounds from the fungi"Abstract of 3^<rd>Pan Pacific Pestiside Science, (in press).

Kimura,Y.、Kusano,M.、Nakahara,S.：“来自真菌的杀线虫化合物”第 3 届泛太平洋农药科学摘要，（正在印刷中）。

Kusano, M.: "βγ-Dehydrocurvuralin and related compounds as nematicides of Pratylenchus penetrans from the fungus Aspergillus sp."Biosci. Biotechnol. Biochem. 67. 1413-1416 (2003)

Kusano，M.：“βγ-脱氢曲夫拉林和相关化合物作为来自真菌曲霉属的短体线虫的杀线虫剂。”Biosci. Biochem. 67. 1413-1416 (2003)

Kimura, Y.: "Nematicidal compounds from the fungi"Abstract of 3^<rd> Pan Pacific Conference. (in press). (2003)

Kimura, Y.：“来自真菌的杀线虫化合物”第三届泛太平洋会议摘要。

Kusano,M., Nakagami,K., Fujioka,S., Kawano,T., Shimada,A., Kimura,Y.: "βγ-Dehydrocurvularin and reloated compounds as nematicide of Pratylenchus penetrans from the fungus Asergillus sp"Biosci.Biotech.Boichem.. 67. 1413-1416 (2003)

Kusano, M.、Nakagami, K.、Fujioka, S.、Kawano, T.、Shimada, A.、Kimura, Y.：“βγ-脱氢曲霉菌素和相关化合物作为来自真菌 Asergillus sp 的穿透短体线虫的杀线虫剂”Biosci。生物技术.Boichem.. 67. 1413-1416 (2003)

M.Kusano, Y.Kimura et al.: "β、γ-Dehydrocurvuralin and related compounds as nematicides of Pratylencus penetrans from the fungus Aspergillus sp."Bioscience, Biotechnology and Biochemistry. 66(in press). (2003)

M.Kusano，Y.Kimura 等人：“β，γ-脱氢曲霉素和相关化合物作为曲霉属短体线虫的杀线虫剂”。《生物科学、生物技术和生物化学》66（出版中）。