Isolation and application of new microorganisms from soil Samples

土壤样品中新微生物的分离及应用

• 批准号: 13836010
• 负责人: TAKAHASHI Yoko
• 金额: $ 2.37万
• 依托单位: Kitasato University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13836010/
• 关键词: Micobial resources; Unknown microorganisms; Superoxide; Superoxide dismutase; Catalase; New bioactive compounds; Inducers of microbial growth; SOD; 休眠細胞活性化因子; グラム陽性高G+Cバクテリア

To increase the possibility of discovering useful new bioactive compounds from microorganisms, we have attempted to isolate new microbial strains by adding various substances to the isolation agar media. In the course of searching for factors necessary for bacterial colony growth, we have found that the predominant bacterial strains release proteins into the culture broths that increase the number of bacterial colonies from a soil sample. Surprisingly, the common protein isolated from the active culture troths was superoxide dismutase (SOD). Moreover, commercial SODs increased the number of colonies, a response further enhanced by the addition of catalase. Superoxide (O_2) was quantitatively detected in the isolation agar medium and the level was suppressed by adding SOD or SOD plus catalase. Our results strongly suggest that some of the bacterial cells may be recovered by suppressing oxidative stress on the agar-based medium.

为了增加从微生物中发现有用的新生物活性化合物的可能性，我们尝试通过在分离琼脂培养基中添加各种物质来分离新的微生物菌株。在寻找细菌菌落生长的必要因素的过程中，我们发现优势菌株释放蛋白质到培养液中，增加了土壤样品中细菌菌落的数量。令人惊讶的是，从活性培养液中分离出的常见蛋白质是超氧化物歧化酶（SOD）。此外，商业sod增加了菌落数量，过氧化氢酶的添加进一步增强了这种反应。在分离琼脂培养基中定量检测超氧化物（O_2），并通过添加SOD或SOD +过氧化氢酶抑制其水平。我们的研究结果强烈表明，一些细菌细胞可能通过抑制琼脂基培养基上的氧化应激而恢复。

项目成果

余 利岩, 高橋洋子, 松本厚子, 清野昭雄, 岩井 譲, 大村 智: "Application of PCR for selection of Gram-positive bacteria with high DNA G+C content among new isolate."Actinomycetologica. 16. 1-5 (2002)

Riiwa Yo、Yoko Takahashi、Atsuko Matsumoto、Akio Seino、Yuzuru Iwai、Satoshi Omura：“PCR 在新分离株中选择具有高 DNA G+C 含量的革兰氏阳性菌的应用。”Actinomycetologica. 16. 1-5 ( 2002 年) ）

松本厚子, 高橋洋子, 篠瀬まゆみ, 清野昭雄, 岩井 譲, 大村 智: "Longispora albida gen. nov., Sp. nov., a novel genus of the family Micromonosporaceae."Int.J.Syst.Evol.Microbiol.. 53. 1553-1559 (2003)

Atsuko Matsumoto、Yoko Takahashi、Mayumi Shinose、Akio Kiyono、Yuzuru Iwai、Satoshi Omura：“Longispora albida gen. nov.、Sp. nov.，小单孢菌科的一个新属。”Int.J.Syst.Evol.Microbiol。 . 53. 1553-1559 (2003)

高橋洋子, 大村 智: "Isolation of new actinomycete strains for the screening of new bioactive compounds."J.Gen.Appl.Microbiol.. 49. 141-154 (2003)

Yoko Takahashi、Satoshi Omura：“分离新的放线菌菌株以筛选新的生物活性化合物。”J.Gen.Appl.Microbiol.. 49. 141-154 (2003)

高橋洋子, 加藤省吾, 志倉教保, 供田 洋, 大村 智: "Superoxide dismutase produced by soil bacteria increases bacterial colony growth from soil samples."J.Gen.Appl.Microbiol.. 49. 263-266 (2003)

Yoko Takahashi、Shogo Kato、Noriyasu Shikura、Hiroshi Toda、Satoshi Omura：“土壤细菌产生的超氧化物歧化酶可促进土壤样品中细菌菌落的生长。”J.Gen.Appl.Microbiol.. 49. 263-266 (2003)

L.Yu, Y.Takahashi, A.Matsumoto, A.Seino, Y.Iwai, S.Omura: "Application of PCR for selection of Grampositive bacteria with high DNA G+C content among new isolate"Actinomycetol.. 16. 1-5 (2002)

L.Yu、Y.Takahashi、A.Matsumoto、A.Seino、Y.Iwai、S.Omura：“PCR 在新分离株中选择具有高 DNA G C 含量的革兰氏阳性菌的应用”Actinomycetol.. 16. 1-5