Novel angiogenic therapy using siRNA-loaded Bubble liposomes with ultrasound exposure

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Recently, we developed polyethyleneglycol(PEG)-modified liposomes(Bubble liposomes ; BLs) entrapping an ultrasound(US) imaging gas. In this study, we prepared novel siRNA-loaded BLs(si-BLs) using cationic lipids, DOTAP, DSTAP, DSDAP, and DDAB, and examined the usability of those BLs as a carrier and an US contrast agent. We demonstrated that siRNA could be loaded onto BLs and that siRNA-loaded BLs was stable in serum. A specific gene-silencing effect was also achieved by transfection with si-BLs. We also could optimize the lipid component of BLs in terms of the US imaging, the gene transfer efficiency, and toxicity. Furthermore, BLs injected into tail vein could reached to ischemic hindlimb of model mice. These results suggest that this method could be a useful tool for angiogenic therapy.