Involvement of neuropeptides in the hypothalamus and limbic system in the adaptation of stress response

下丘脑和边缘系统中的神经肽参与应激反应的适应

• 批准号: 13670076
• 负责人: NAGATA Shoji
• 金额: $ 1.86万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670076/
• 关键词: stress response; hypothalamic paraventricular nucleus; hypothalamic-pituitary-adrenal axis; adaptation; c-fos gene expression; neuropeptides; immunity; corticosterone; immediate early gene (IEG); ストレス反応; 内分泌; 免疫細胞; immediate early gene; 新奇ストレ

In order to clarify the processes of adaptation to stress in hypothalamic pituitary adrenal (HPA) axis and neuroendocrinoimmune system, three experiments were performed using Wister male rats.The results were following.1. A long-duration restraint stress for 16 hours evoked adaptive change, characterized by transient increase and gradual recovery to basal level in c-fos mRNA/Fos protein expression in the hypothalamic paraventricular nucleus (PVN) and in plasma adrenocorticotropin (ACTH) levels, and transient decrease following slow recovery in a number of total white blood cells and some lymphocytes subpopulations, although circulating corticosterone (CORT) remained at high level.2. Superimposed cold stress-induced re-expression of c-fos mRNA/Fos in the parvocellular region of the PVN was observed in 16 hours restraint rats. The degree of expression of c-fos gene in superimposed cold stress rats was the same as those observed in single cold stress rats (normo-responsive). The plasma AC … More TH levels in superimposed cold stress rats were significantly higher compared with those in single cold stress rats (hyper-responsive, facilitation). There was no significant differences between plasma CORT level s superimposed cold stress rats and those in single cold stress rats (not hyper-responsive).3. The plasma ACTH and CORT levels, c-fos mRNA and AVP heteronuclear RNA expression in the PVN in the repeated restraint stress (1 hour/day) rats showed the decreasing tendency (habituation) at 7 days and 14 days later comparing with those at the first experiment day. On the other hand, the habituation of these responses was not observed in repeated cold (4℃)+restraint stress rats.These results suggest that adaptation of stress response may occur in various stressful situation and the stress response to a superimposed stressor may occur in a different way at each level of the HPA axis, and that occurrence of habituation or facilitation of stress response to repeated stressors may related the sort of stressors. Less

为了阐明下丘脑-垂体-肾上腺（HPA）轴和神经内分泌-免疫系统对应激的适应过程，本研究采用Wister雄性大鼠进行了三个实验，结果如下：1.长时间束缚应激16小时引起适应性变化，其特征是下丘脑室旁核（PVN）c-fos mRNA/Fos蛋白表达和血浆促肾上腺皮质激素（ACTH）水平短暂升高并逐渐恢复到基础水平，而总白色血细胞和某些淋巴细胞亚群的表达短暂下降并缓慢恢复。尽管循环皮质酮（CORT）仍处于较高水平.在束缚16小时的大鼠中观察到叠加冷应激诱导的室旁核小细胞区c-fos mRNA/Fos的再表达。c-fos基因在复合冷应激大鼠中的表达程度与单纯冷应激大鼠（正常反应）相同。等离子AC ...更多信息 复合冷应激组TH水平显著高于单纯冷应激组（高反应性、易化性）。复合冷应激大鼠血浆CORT水平与单纯冷应激大鼠（无高反应性）无显著性差异.重复束缚应激（1h/d）大鼠血浆ACTH、CORT水平、室旁核c-fos mRNA和AVP mRNA表达在7 d和14 d时均呈下降趋势（习惯化）。而在反复冷（4℃）+束缚应激大鼠中则未观察到这些反应的习惯化，提示应激反应的适应性可能发生在不同的应激情境中，且对叠加应激的应激反应在HPA轴的各个水平上可能以不同的方式发生，应激反应的适应化或易化可能与应激源的种类有关。少

项目成果

Irie, M., Maeda, M, Nagata, S.: "Can conditioned histamine release occur under urethane anesthesia in guinea pigs?"Physiology & Behavior. 72. 567-573 (2001)

Irie, M.、Maeda, M、Nagata, S.：“在乌拉坦麻醉下，豚鼠会发生条件性组胺释放吗？”生理学

Kurose, T., Ueta, Y., Yamamoto, Y., Serino, R., Ozaki, Y., Saitoh, J., Nagata, S., Yamashita, H.: "Effects of restricted feeding on the activity of hypothalamic orexin (OX)-A containing neurons and OX2 receptor mRNA level in the paraventricular nucleus of

Kurose, T.、Ueta, Y.、Yamamoto, Y.、Serino, R.、Ozaki, Y.、Saitoh, J.、Nagata, S.、Yamashita, H.：“限制进食对下丘脑活动的影响

Tan, Z: "PVN c-fos expression, HPA axis response and immune cell distribution during restraint stress"J UOEH. 24・2. 131-149 (2002)

Tan，Z：“束缚应激期间PVN c-fos表达、HPA轴反应和免疫细胞分布”J UOEH 24・2（2002）。

Kurose, T., Ueta, Y., Nomura, M., Yamaguchi, K., Nagata, S.: "Nociceptive stimulation Increases NO synthase mRNA and vasopressin heteronuclear RNA in the rat paraventricular nucleus"Autonomic Neuroscience : Basic and Clinical. 88・1-2. 52-60 (2001)

Kurose, T.、Ueta, Y.、Nomura, M.、Yamaguchi, K.、Nagata, S.：“伤害感受刺激增加大鼠室旁核中的 NO 合酶 mRNA 和加压素异核 RNA”自主神经科学：基础和临床 88。・1-2。52-60（2001）

Irie, M: "Can conditioned histamine release occur under urethane anesthesia in guinea pigs?"Physiology & Behavior. 72. 567-573 (2001)

Irie, M：“豚鼠在乌拉坦麻醉下会发生条件性组胺释放吗？”