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An investigation of the relationship between oxidative DNA damage and psychological stress or life style - using urinary 8-OHdG

利用尿 8-OHdG 研究氧化 DNA 损伤与心理压力或生活方式之间的关系

基本信息

批准号：
16590565
负责人：
NAGATA Shoji
金额：
$ 2.24万
依托单位：
University of Occupational and Environmental Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

It has been thought that 8-Hydroxydeoxyguanosine (8-OHdG) is one of the important indicators of oxidative DNA damage. The project consisted of 3 studies as following.1.Investigation of the relationship between job stress and 8-OHdG was carried out for 92 employees in 2002 and 2003. The range of variation in urinary 8-OHdG/Creatinine (8-OHdG) was very high, however the intrapersonal variation was very low. The correlation coefficient between 8-OHdG in 2002 and 2003 was 0.850, which was significant at 0.01 level. The 8-OHdG of smokers was significantly higher than those of non smokers. As regard to job stress, significant negative correlations between job control, vigor, anxiety, job or life satisfaction and 8-OHdG were observed.2.In order to investigate the influence of stress on oxidative DNA damage, urine and blood of 10 male medical students in 4th grade (examination group) were collected at 1 month before and 3 days after start of examination series. 11 male students in 3rd grade we … More re chosen for the control group, who had no examination. The examination group showed significantly high GHQ scores and tensions, anxiety, fatigue score and significantly lower vigor score in POMS and no significant increase of 8-OHdG and plasma cortisol comparing to the control group, while urinary DHEA-S increased significantly during examination.3.We investigated the relationship between shift work and oxidative DNA damage, with cooperation of 10 shift workers in a manufacturing company. Salivary cortisol, 8-OHdG and POMS were measured at 7:00 (after midnight work), 15:00 (after daytime work) and 23:00 (after night work) and at the same time in holiday. Although circadian rhythm was observed in salivary cortisol, there was no significant differences between working day and holiday. As regard to 8-OHdG, significant difference was not found among 3 working hours, however 8-OHdG was significantly higher in holiday. No significant difference was found in the fatigue, anxiety, depression, vigor scores in POMS among 3 working hours. Less

8-羟基脱氧鸟苷（8-OHdG）被认为是DNA氧化损伤的重要指标之一。本研究包括以下三个方面的研究：1.于2002年和2003年对92名企业员工进行了工作压力与8-羟脱氧葡萄糖（8-OHdG）的关系调查。尿8-OHdG/肌酐（8-OHdG/Creatinine，8-OHdG/Creatinine）的变异范围很大，但个体内变异很小。2002年与2003年8-OHdG含量的相关系数为0.850，达到极显著水平。吸烟者的8-OHdG显著高于非吸烟者。在工作压力方面，工作控制感、活力、焦虑、工作或生活满意度与8-OHdG呈显著负相关。2.为探讨工作压力对DNA氧化损伤的影响，对10名四年级男生（考试组）在考试前1个月和考试后3 d分别采集尿液和血液，进行DNA氧化损伤的检测。三年级11名男生， ...更多信息另设对照组，不做任何检查。与对照组相比，检查组GHQ评分、紧张、焦虑、疲劳评分显著升高，POMS活力评分显著降低，而8-OHdG和血浆皮质醇水平无显著升高，尿DHEA-S水平显著升高。分别于7：00（夜班后）、15：00（白班后）、23：00（夜班后）和节假日同一时间测定唾液皮质醇、8-OHdG和POMS。唾液皮质醇含量虽有昼夜节律，但工作日与节假日无明显差异。8-OHdG在3个工作小时之间差异不显著，而在假期中显著升高。3个工作时间组POMS中疲劳、焦虑、抑郁、精力四项得分差异无统计学意义。少