An investigation of the relationship between oxidative DNA damage and psychological stress or life style - using urinary 8-OHdG

8-羟基脱氧鸟苷（8-OHdG）一直被认为是DNA氧化损伤的重要指标之一。本课题包括以下3项研究：1.研究内容：本研究于2002年和2003年对92名员工进行了工作压力与8-OHdG的关系调查。尿8-OHdG/肌酐（8-OHdG）的变化范围非常大，但个人间的变化非常小。2002年和2003年8-OHdG的相关系数为0.850，在0.01水平上显著。吸烟者的8-OHdG明显高于非吸烟者。在工作压力方面，工作控制、活力、焦虑、工作或生活满意度与8-OHdG呈显著负相关。为探讨应激对氧化性DNA损伤的影响，收集了10名四年级男医学生（试验组）在系列检查开始前1个月和开始后3天的尿液和血液。11名三年级男生被重新选为对照组，他们没有考试。2 .检查组GHQ评分显著高于对照组，POMS紧张、焦虑、疲劳评分显著高于对照组，活力评分显著低于对照组，8-OHdG和血浆皮质醇无显著升高，尿DHEA-S显著升高。我们在一家制造公司的10名轮班工人的合作下，调查了轮班工作与氧化性DNA损伤的关系。分别于7:00（午夜工作后）、15:00（白天工作后）、23:00（夜间工作后）及假期同一时间测量唾液皮质醇、8-OHdG和POMS。虽然在唾液皮质醇中观察到昼夜节律，但工作日和假期之间没有显著差异。8-OHdG在3个工作时间间无显著差异，但假期8-OHdG显著增高。3个工作时间间，POMS的疲劳、焦虑、抑郁、活力得分均无显著差异。少

It has been thought that 8-Hydroxydeoxyguanosine (8-OHdG) is one of the important indicators of oxidative DNA damage. The project consisted of 3 studies as following.1.Investigation of the relationship between job stress and 8-OHdG was carried out for 92 employees in 2002 and 2003. The range of variation in urinary 8-OHdG/Creatinine (8-OHdG) was very high, however the intrapersonal variation was very low. The correlation coefficient between 8-OHdG in 2002 and 2003 was 0.850, which was significant at 0.01 level. The 8-OHdG of smokers was significantly higher than those of non smokers. As regard to job stress, significant negative correlations between job control, vigor, anxiety, job or life satisfaction and 8-OHdG were observed.2.In order to investigate the influence of stress on oxidative DNA damage, urine and blood of 10 male medical students in 4th grade (examination group) were collected at 1 month before and 3 days after start of examination series. 11 male students in 3rd grade we … More re chosen for the control group, who had no examination. The examination group showed significantly high GHQ scores and tensions, anxiety, fatigue score and significantly lower vigor score in POMS and no significant increase of 8-OHdG and plasma cortisol comparing to the control group, while urinary DHEA-S increased significantly during examination.3.We investigated the relationship between shift work and oxidative DNA damage, with cooperation of 10 shift workers in a manufacturing company. Salivary cortisol, 8-OHdG and POMS were measured at 7:00 (after midnight work), 15:00 (after daytime work) and 23:00 (after night work) and at the same time in holiday. Although circadian rhythm was observed in salivary cortisol, there was no significant differences between working day and holiday. As regard to 8-OHdG, significant difference was not found among 3 working hours, however 8-OHdG was significantly higher in holiday. No significant difference was found in the fatigue, anxiety, depression, vigor scores in POMS among 3 working hours. Less