Involvement of limbic system and hypothalamus in bronchial allergic reaction

我们在前期研究中报道了电解产生的双侧下丘脑前部损伤（AHL）对豚鼠和大鼠气道即刻型和延迟型过敏反应的抑制作用，以及豚鼠条件组胺释放的机制。本实验旨在探讨微注射桂酸代替电解产生的AHL对大鼠气道过敏反应的抑制作用，以及AHL对下丘脑区神经细胞的激活作用，并探讨脑内条件组胺释放的机制。1)在双侧下丘脑内侧视前区注射kainic酸的MPO组，与非MPO组和假手术组相比，采用更多显微注射方式的MPO组，其肺组织嗜酸性粒细胞（BALF）和肺组织嗜酸性粒细胞（抗原激发后晚期过敏反应的指标）均显著降低。上述结果提示，本研究方法制备的AHL可抑制大鼠气道的晚期过敏反应。术后7 d，三组大鼠脑室周围下丘脑区（PVH）、杏仁核等脑区神经细胞Fos蛋白表达差异无统计学意义。我们打算研究抗原激发后下丘脑c-fos mRNA的表达以及从手术到脑组织固定的最佳时间。2)观察条件组和非条件组致敏大鼠在条件刺激30 min后脑神经细胞中c-fos mRNA的表达。虽然在PVH中观察到c-fos mRNA的弱表达，但两组间无显著差异。我们目前正在研究原位杂交方法的调理方法和规程。少

We have reported in previous studies the inhibition of the immediate and delayed type allergic reactions in the airway of guinea pigs and rats by the bilateral anterior hypothalamic lesioning (AHL) produced by electrolysis, and the mechanism of conditioned histamine release in guinea pigs. In this study, we carried out experiments in order to investigate whether the inhibition of allergic reaction in the rat's airway may or may not be caused by the AHL produced by microinjection of kainic acid instead of electotrlysis and the activation of the nerve cells in the hypothalamic area by the AHL, and to investigate the mechanism in the brain regarding conditioned histamine release.Results. 1) Both the eosinophils in bronchoalbeolar lavage fluids (BALF) and lung tissues, which are the indicators of the late allergic reaction after the antigen challenge, were significantly lower in the MPO group that was injected with kainic acid into the bilateral medial preoptic area of the hypothalamus by … More microinjection methods, when compared with those of the non-MPO group and sham-operation group. These results suggest that AHL produced by the method used in this study may inhibit the late allergic reaction in the airway of rats.There were no significant differences in the expression of Fos protein in the nerve cells at the periventricular hypothalamic area (PVH), amygdala and other areas in the brain 7 days after the operation among the three groups. We intend to investigate the expression of c-fos mRNA in the hypothalamic area after the antigen challenge and fhe optimal duration from the operation to the fixation of brain tissue.2) The c-fos mRNA expression in the brain nerve cells was investigated 30 minutes after the conditioned stimulus in both the conditioned and unconditioned groups of sensitized rats. Although a weak expression of c-fos mRNA in the PVH was observed, no significant difference was found between both groups. We are now investigating the methods of the conditioning and the protocols of in situ hybridization method. Less