Involvement of limbic system and hypothalamus in bronchial allergic reaction

边缘系统和下丘脑参与支气管过敏反应

• 批准号: 09670501
• 负责人: NAGATA Shoji
• 金额: $ 1.47万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1998
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670501/
• 关键词: allergic reaction; limbic system; hypothalamus; conditioming; histanine release; anterior hypothalamic lesion; c-fos mRNA; Fos protein; c-fos m RNA発現; 気道反応; Fos蛋白; 気管支肺胞洗浄液; 好酸球; マイクロインジェクション

We have reported in previous studies the inhibition of the immediate and delayed type allergic reactions in the airway of guinea pigs and rats by the bilateral anterior hypothalamic lesioning (AHL) produced by electrolysis, and the mechanism of conditioned histamine release in guinea pigs. In this study, we carried out experiments in order to investigate whether the inhibition of allergic reaction in the rat's airway may or may not be caused by the AHL produced by microinjection of kainic acid instead of electotrlysis and the activation of the nerve cells in the hypothalamic area by the AHL, and to investigate the mechanism in the brain regarding conditioned histamine release.Results. 1) Both the eosinophils in bronchoalbeolar lavage fluids (BALF) and lung tissues, which are the indicators of the late allergic reaction after the antigen challenge, were significantly lower in the MPO group that was injected with kainic acid into the bilateral medial preoptic area of the hypothalamus by … More microinjection methods, when compared with those of the non-MPO group and sham-operation group. These results suggest that AHL produced by the method used in this study may inhibit the late allergic reaction in the airway of rats.There were no significant differences in the expression of Fos protein in the nerve cells at the periventricular hypothalamic area (PVH), amygdala and other areas in the brain 7 days after the operation among the three groups. We intend to investigate the expression of c-fos mRNA in the hypothalamic area after the antigen challenge and fhe optimal duration from the operation to the fixation of brain tissue.2) The c-fos mRNA expression in the brain nerve cells was investigated 30 minutes after the conditioned stimulus in both the conditioned and unconditioned groups of sensitized rats. Although a weak expression of c-fos mRNA in the PVH was observed, no significant difference was found between both groups. We are now investigating the methods of the conditioning and the protocols of in situ hybridization method. Less

我们在以前的研究中已经报道了电解产生的双侧下丘脑前部损毁(AHL)对豚鼠和大鼠呼吸道即刻和迟发性变态反应的抑制作用，以及豚鼠条件性组胺释放的机制。在本研究中，我们进行了实验研究，以探讨微量注射红藻氨酸而不是电刺激产生的AHL和AHL激活下丘脑区神经细胞是否导致大鼠呼吸道过敏反应的抑制，并探讨其在脑内条件性组胺释放的机制。1)经…双侧下丘脑内侧视前内侧区注射海人酸组，抗原攻击后晚期过敏反应指标--支气管肺泡灌洗液和肺组织中的嗜酸性粒细胞均显著降低。显微注射方法较非MPO组和假手术组多。结果表明，本实验方法制备的AHL可抑制大鼠呼吸道晚期变态反应。术后7天，3组大鼠下丘脑室周区、杏仁核等脑区神经细胞Fos蛋白的表达差异无统计学意义。2)观察条件性和非条件性致敏大鼠在条件性刺激后30min脑神经细胞c-fos mRNA的表达。虽然c-fos mRNA在PVH中有微弱表达，但两组间无显著差异。我们正在研究原位杂交法的条件方法和操作规程。较少

项目成果

Inoue M, Maeda M et al: "Regional differentiation of blood flow response to microinjection of sodium nitroprusside" J Autonomic Nervous System. 63(3). 172-178 (1997)

Inoue M、Maeda M 等人：“微量注射硝普钠的血流反应的区域分化”J Autonomic Nervous System。

永田 頌史: "気管支喘息の心身医療 気管支喘息の成り立ち-(1)アレルギー学的・精神神経免疫学的考え方" 桂載作、吾郷晋浩(編), 250 (1997)

Shoji Nagata：“支气管哮喘的心理物理医学。支气管哮喘的起源 - (1) 过敏学和心理神经免疫学概念” Katsura Saisaku，Ago Shinhiro（编辑），250（1997）

永田頌史: "心身症における器官選択-気管支喘息について" 心身医学. 39(2). 127-136 (1999)

Yoji Nagata：“心身疾病的器官选择 - 关于支气管哮喘”心身医学 39(2)。

Nagata S: "Involvement of nervous system in immune and allergic reaction" Arerugika. 5. 24-30 (1998)

Nagata S：“神经系统参与免疫和过敏反应”Arerugika。

永田頌史: "ストレスの基礎研究の現状-医学・生理学(分担執筆)ストレス研究の基礎と臨床(現代のエスプリ別冊)" 河野反信、石川俊男(編)(印刷中), (1999)

Shoshi Nagata：“压力基础研究的现状 - 医学和生理学（合著者）基础和临床压力研究（现代 Esprit 特别版）” Tananobu Kono、Toshio Ishikawa（编辑）（出版中），（1999 年）