Cartilage Tissue Engineering Using Chondrogenic Cell Line N1511

使用软骨细胞系 N1511 进行软骨组织工程

• 批准号: 13670231
• 负责人: WATANABE Hideto
• 金额: $ 2.3万
• 依托单位: Aichi Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670231/
• 关键词: cartilage; chondrocyte; cell line; differentiation; tissue engineering; p53; knockout mouse; アグリカン; バーシカン; プロテオグリカン; 細胞外マトリックス; 再生医学

The aim of the project was to develop a method of the cartilage tissue engineering using a chondrogenic cell line N1511, which had been established from chondrocytes of a p53-null mouse.First, we characterized N1511 cells. Induction of chondrocyte differentiation was attained when the cells were treated with bone morphogenetic proteins (BMPs) and insulin, or parathyroid hormone and dexamethasone. Recovery of p53 expression in N1511 cells inhibited cell proliferation, suggesting that the cell proliferation could be regulated with p53 in this cell line. These results indicate that N1511 undergoes multiple steps of chondrocyte differentiation and because proliferation could be regulated by expression of p53, N1511 could be an excellent model for studies of chondrogenesis, the function of p53, and genetic engineering of cartilage tissue. We are currently studying the roles of various proteoglycans in chondrocyte differentiation, using this cell line.We also performed two studies related to this project: The effect of ultrasound on chondrocyte differentiation of mesenchymal stem cells, and characterization of an anti-decorin mouse monoclonal antibody 6B6. The results of these studies will facilitate studies on chondrocyte differentiation and cartilage tissue engineering.

本课题的目的是利用p53基因敲除小鼠的软骨细胞建立成软骨细胞系N1511，建立软骨组织工程的方法。软骨细胞分化的诱导时，细胞与骨形态发生蛋白（BMP）和胰岛素，或甲状旁腺激素和地塞米松处理。在N1511细胞中p53表达的恢复抑制细胞增殖，表明在该细胞系中p53可以调节细胞增殖。这些结果表明，N1511经历了多个步骤的软骨细胞分化，因为增殖可以通过p53的表达来调节，N1511可以成为研究软骨发生、p53的功能和软骨组织基因工程的良好模型。我们目前正在使用该细胞系研究各种蛋白聚糖在软骨细胞分化中的作用。我们还进行了与该项目相关的两项研究：超声波对间充质干细胞软骨细胞分化的影响，以及抗核心蛋白聚糖小鼠单克隆抗体6B6的表征。这些研究结果将有助于软骨细胞分化和软骨组织工程的研究。

项目成果

Sawada, H.: "Characterization of an anti-decorin monoclonal antibody, and its utility"J Biochem. 132. 997-1002 (2002)

Sawada, H.：“抗核心蛋白聚糖单克隆抗体的表征及其实用性”J Biochem。

Watanabe, H., and Kimata, K.: "Knockout mice of proteoglycans"The Cell. 34-2. 64-67 (2002)

Watanabe, H. 和 Kimata, K.：“蛋白多糖敲除小鼠”细胞。

Kamiya, N.: "Establishment of a novel chondrocytic Cell line N1511 derived from P53-null mice"J Bone Miner Res. 17. 1832-1842 (2002)

Kamiya, N.：“源自 P53 缺失小鼠的新型软骨细胞系 N1511 的建立”J Bone Miner Res。

渡辺 秀人: "ポストゲノム時代の糖鎖生物学がわかる(わかる実験医学シリーズ)"羊土社. 131 (2002)

渡边秀人：“理解后基因组时代的糖生物学（理解实验医学系列）”Yodosha 131（2002）。

Ebisawa, K., et al.: "Cartilage tissue engineering"Biomaterial. in press. (2002)

Ebisawa, K., et al.：“软骨组织工程”生物材料。