MATRILYSIN AS A MARKER FOR AGGRESSIVE GASTROINTESTINAL CANCER
MATRILYSIN 作为侵袭性胃肠癌的标志物
基本信息
- 批准号:13670532
- 负责人:
- 金额:$ 2.24万
- 依托单位:
- 依托单位国家:日本
- 项目类别:Grant-in-Aid for Scientific Research (C)
- 财政年份:2001
- 资助国家:日本
- 起止时间:2001 至 2002
- 项目状态:已结题
- 来源:
- 关键词:
项目摘要
Expression of E1AF/PEA3 (ETV4), an ets family transcriptional factor, has been implicated in invasive potential of several cancer cell lines through induction of matrix metalloproteinase (MMP) expression. The aim of this study was to examine E1AF mRNA expression and determine whether it is correlated with the progression and/or MMP expression in human colorectal cancer. Using the semiquantitative reverse transcriptase-polymerase chain reaction (RT-PCR), we analyzed 100 colorectal cancer tissues for E1AF mRNA expression. Expression of ER81 (ETV1) and ERM (ETV5), the other 2 members of the PEA3 subfamily, and Ets-1 and Ets-2 was also analyzed. The results were correlated with clinicopathological characteristics and MMP expression. Immunohistochemical analysis and in vitro invasion assay were also performed. E1AF mRNA expression was detected in 62% of the 100 colorectal cancer tissues but was undetectable or only faintly detected in adjacent nontumorous tissues. E1AF mRNA was detected in … More all of the 10 liver metastatic tumor tissues from colorectal cancers. E1AF expression was significantly correlated with depth of invasion, lymphatic and venous invasion, lymph node and distant metastasis, advance in pathological tumor-node-metastasis stage, and recurrence. Patients with E1AF-positive cancer had significantly shorter overall and disease-free survival periods than did those with E1AF-negative cancer (P<0.0001 and P<0.0001, respectively). E1AF expression retained its significant predictive value for overall and disease-free survival in multivariate analysis that included conventional clinicopathological factors (P=0.0066 and P=0.0109, respectively). Among the expression of MMPs analyzed, expression of MMP-1 and matrilysin was significantly correlated with E1AF expression. In contrast, expression of ER81 and ERM was not correlated with clinicopathological characteristics or expression of these MMPs. Immunohistochemical expression of E1AF was predominantly observed at the invasive front, where the expression of MMP-1 and matrilysin and nuclear b-catenin expression were often colocalized. Less
E1 AF/PEA 3(ETV 4)是ets家族转录因子,其表达通过诱导基质金属蛋白酶(MMP)表达而与多种癌细胞系的侵袭潜能有关。本研究的目的是检测E1 AF mRNA的表达,并确定其是否与人类结直肠癌的进展和/或MMP表达相关。采用半定量逆转录聚合酶链反应(RT-PCR)方法检测100例大肠癌组织中E1 AF mRNA的表达。还分析了PEA 3亚家族的另外2个成员ER 81(ETV 1)和ERM(ETV 5)以及Ets-1和Ets-2的表达。结果与临床病理特征及MMP表达相关。免疫组化分析和体外侵袭实验也进行了。100例结直肠癌组织中E1 AF mRNA表达率为62%,而癌旁组织中E1 AF mRNA表达率不高或较低。E1 AF mRNA在 ...更多信息 所有10个来自结直肠癌的肝转移肿瘤组织。E1 AF表达与肿瘤浸润深度、淋巴结和静脉浸润、淋巴结转移、远处转移、病理分期、复发密切相关。与E1 AF阴性癌症患者相比,E1 AF阳性癌症患者的总体生存期和无病生存期显著缩短(分别为P<0.0001和P<0.0001)。在包括常规临床病理因素的多变量分析中,E1 AF表达对总生存期和无病生存期具有显著的预测价值(分别为P=0.0066和P=0.0109)。在分析的MMPs表达中,MMP-1和matrilysin的表达与E1 AF表达显著相关。相反,ER 81和ERM的表达与临床病理特征或这些MMPs的表达无关。E1 AF的免疫组化表达主要见于浸润前沿,MMP-1和matrilysin的表达以及核内b-连环蛋白的表达常共存于浸润前沿。少
项目成果
期刊论文数量(4)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
Itoh F: "Aberrant methylation and histone deacetylation of cyclooxy genese 2 in gastric cancer"Int. J. Cancer. 97・3. 272-277 (2002)
Itoh F:“胃癌中环氧基基因 2 的异常甲基化和组蛋白脱乙酰化”Int. Cancer 97・3 (2002)。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Kikuchi T: "Regional methylation and histone deacethylation of p57KIP2 associated with Gene silencing in human tumors"Oncogene. (in-press). (2002)
Kikuchi T:“p57KIP2 的区域甲基化和组蛋白脱乙酰化与人类肿瘤中的基因沉默相关”癌基因。
- DOI:
- 发表时间:
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- 影响因子:0
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- 通讯作者:
Yaniamoto H: "Differential involvement of the hypermethylator phenotype in hereditary and sporadic colorectal cancers with high-frequency microsatellite instability"Genes Chromosomes Cancer. (in press). (2002)
Yaniamoto H:“高甲基化表型在具有高频微卫星不稳定性的遗传性和散发性结直肠癌中的不同参与”基因染色体癌症。
- DOI:
- 发表时间:
- 期刊:
- 影响因子:0
- 作者:
- 通讯作者:
Sasaki Y, Itoh F et al.: "Increased expression of t-fimbrin gene after DNA damage in cho cells and inactivation of t-fimbrin by CpG methylation in human colorectal cancer cells"Int J Cancer. 97. 211-216 (2002)
Sasaki Y、Itoh F 等人:“cho 细胞中 DNA 损伤后 t-fimbrin 基因的表达增加,以及人结直肠癌细胞中 CpG 甲基化导致 t-fimbrin 失活”Int J Cancer。
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- 影响因子:0
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{{ truncateString('ITOH Fumio', 18)}}的其他基金
Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using an MCA-microarray and bisulfate pyrosequencing
使用 MCA 微阵列和硫酸氢盐焦磷酸测序鉴定放化疗前后食管癌 DNA 甲基化变化
- 批准号:
21590797 - 财政年份:2009
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
DEVELOPMENT OF A NOVEL EPIGENETIC SCREENING METHOD FOR DETECTING THE COLORECTAL CANCER
开发一种新的表观遗传学筛查方法来检测结直肠癌
- 批准号:
17390222 - 财政年份:2005
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Analysis of gene expression in GIST by cDNA array
cDNA 芯片分析 GIST 基因表达
- 批准号:
15590663 - 财政年份:2003
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
Research and Development of Cutting-edge Global Financial Techonology
全球前沿金融科技研发
- 批准号:
11430025 - 财政年份:1999
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Research and Education of Finance Using Virtual Financial Market
利用虚拟金融市场进行金融研究和教育
- 批准号:
11695020 - 财政年份:1999
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (B)
Relation of matrixmetalloproteinases and infalammatory bowel disease
基质金属蛋白酶与炎症性肠病的关系
- 批准号:
09670559 - 财政年份:1997
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for Scientific Research (C)
The Global Classroom and the Virtual Financial Market
全球课堂和虚拟金融市场
- 批准号:
08045013 - 财政年份:1996
- 资助金额:
$ 2.24万 - 项目类别:
Grant-in-Aid for international Scientific Research
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8112795 - 财政年份:2010
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- 批准号:
7497481 - 财政年份:2007
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Matrilysin regulation in colonic epithelial cells and role in barrier function
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8131795 - 财政年份:2007
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Matrilysin regulation in colonic epithelial cells and role in barrier function
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7385504 - 财政年份:2007
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Matrilysin regulation in colonic epithelial cells and role in barrier function
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7687573 - 财政年份:2007
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Matrilysin regulation in colonic epithelial cells and role in barrier function
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8053977 - 财政年份:2007
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Matrilysin regulation in colonic epithelial cells and role in barrier function
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