Analysis of gene expression in GIST by cDNA array

cDNA 芯片分析 GIST 基因表达

• 批准号: 15590663
• 负责人: ITOH Fumio
• 金额: $ 2.24万
• 依托单位: ST.MARIANNA UNIVERSITY SCHOOL OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15590663/
• 关键词: GIST; cDNA array; VEGF recepter; Bcl-2; malignant potential; diagnosis; 遺伝子不安定性

It is generally accepted that gastrointestinal stromal tumor(GIST) arise in Cajal cell of intestine. The aim of this study was to clarify relevant alterations of gene expression associated with the malignant progression of gastrointestinal stromal tumor. Using cDNA array, we analysed the expression profiles of 550 cancer-related genes in 30 gastrointestinal stromal tumor (10 malignant GIST and 20 GIST with low grade malignancy). Among the 550 genes, we chose 30 genes the average expression levels of which were at least three-fold up-or downregulated in tumour tissues compared with levels in matched normal tissues. Among the upregulated genes, the average expression levels of VFGF and bcl-2 were over five times higher than those in matched normal tissues. The expression profiles obtained by the cDNA array clearly indicate that malignant GIST have specific expression profiles. These results indicate that molecular classification of malignant potential of gastrointestinal stromal tumor by a cDNA array is feasible.

胃肠道间质瘤（GIST）发生于小肠Cajal细胞，是公认的肿瘤类型.本研究的目的是阐明与胃肠道间质瘤恶性进展相关的基因表达变化。应用cDNA微阵列技术分析了30例胃肠道间质瘤（恶性10例，低度恶性20例）中550个癌相关基因的表达谱。在550个基因中，我们选择了30个基因，与匹配的正常组织相比，这些基因的平均表达水平在肿瘤组织中上调或下调至少三倍。在上调的基因中，VFGF和bcl-2的平均表达水平比匹配的正常组织高5倍以上。由cDNA阵列获得的表达谱清楚地表明恶性GIST具有特异性表达谱。这些结果表明，用cDNA芯片对胃肠道间质瘤恶性潜能进行分子分型是可行的。

项目成果

NAIKAGAKU.

内化学。

• 发表时间: 2003
• 期刊: NAIKAGAKU
• 作者: YAMAMOTO H;IKU S;ITOH F;et al.;ITOH F.
• 通讯作者: ITOH F.

Matsuno K: "The expression of matrix metalloproteinase matrilysin indicates the degree of inflammation in ulcerative colitis."J Gastroenterol. 38. 348-354 (2003)

Matsuno K：“基质金属蛋白酶基质溶解素的表达表明溃疡性结肠炎的炎症程度。”J Gastroenterol。

Toyota M: "Epigenetic inactivation of CHFR in human tumors."Proc Natl Acad Sci USA. 100. 7818-7823 (2003)

Toyota M：“人类肿瘤中 CHFR 的表观遗传失活。”Proc Natl Acad Sci USA。

A full genome scan gastric cancer.

全基因组扫描胃癌。

• 发表时间: 2005
• 期刊: J Med Genet 42
• 作者: AOKI M;ITOH F;et al.;Aoki M
• 通讯作者: Aoki M

内科学

内科

• 发表时间: 2012
• 作者: Matsuzawa R;Takasusuki T;Maekawa M;Yamaguchi S;Hori Y;田中惠子
• 通讯作者: 田中惠子