Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using an MCA-microarray and bisulfate pyrosequencing

使用 MCA 微阵列和硫酸氢盐焦磷酸测序鉴定放化疗前后食管癌 DNA 甲基化变化

• 批准号: 21590797
• 负责人: ITOH Fumio
• 金额: $ 3万
• 依托单位: St. Marianna University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590797/
• 关键词: 食道癌; 治療; 化学療法; 効果予測; 網羅的メチル化解析; 科学療法

Tumor and adjacent normal tissue biopsy specimens were obtained before/after treatment from 34 patients(124 samples) treated with a uniform CRT protocol. We analyzed genomewide DNA methylation of 12 non-treated test samples(T and matched ADJ : 6 each) by methylated CpG island amplification and microarray(MCAM) method, and selected candidate genes that were highly methylated in T compared to ADJ. CYP26C1 was selected by unsupervised hierarchical clustering after MCAM in test samples, and was also ranked in the top 5 of both molecular & cellular function networks as a drug metabolism category. CYP26C1 DNA methylation was significantly higher in T than ADJ in both test and validation samples. CYP26C1 has a CpG island in promoter area, which is highly methylated in T of ESCC patients.

在治疗前/后从34例接受统一CRT方案治疗的患者（124个样本）中获得肿瘤和邻近正常组织活检标本。我们分析了12个未经处理的测试样本的全基因组DNA甲基化，（T和匹配的ADJ：通过甲基化CpG岛扩增和微阵列（MCAM）方法，并选择与ADJ相比在T中高度甲基化的候选基因。在测试样品中的MCAM之后，通过无监督分层聚类选择CYP 26 C1，在药物代谢类的分子和细胞功能网络中也名列前5名。在供试品和验证样品中，T中的CYP 26 C1 DNA甲基化显著高于ADJ。CYP 26 C1基因启动子区存在一个CpG岛，在食管鳞癌患者T细胞中高度甲基化。

项目成果

Prospective study of definitive chemoradiotherapy with S-1 and nedaplatin in patients with stage II/III (non-T4) esophageal cancer

• DOI: 10.1007/s10388-011-0261-0
• 发表时间: 2011-03-01
• 期刊: ESOPHAGUS
• 影响因子: 2.4
• 作者: Tsuda, Takashi;Inaba, Hiroyuki;Itoh, Fumio
• 通讯作者: Itoh, Fumio

Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using a MCA-microarray and bisulfate pyrosequenci

使用 MCA 微阵列和硫酸氢盐焦磷酸序列鉴定放化疗前后食管癌 DNA 甲基化变化

• 发表时间: 2010
• 作者: 津田享志;渡邊嘉行;伊東文生
• 通讯作者: 伊東文生

Gastric Wash-Based Molecular Testing for Antibiotic Resistance in Helicobacter pylori

• DOI: 10.1159/000332570
• 发表时间: 2011-01-01
• 期刊: DIGESTION
• 影响因子: 3.2
• 作者: Baba, Satoshi;Oishi, Yoshichika;Itoh, Fumio
• 通讯作者: Itoh, Fumio

Sensitive and specific detection of early gastric cancer using DNA methylation analysis of gastric washes.

使用胃洗液 DNA 甲基化分析敏感且特异地检测早期胃癌。

• 发表时间: 2009
• 期刊: Gastroenterology 136
• 作者: Yoshiyuki Watanabe;et al.
• 通讯作者: et al.

Identification of DNA methylation changes in esophageal cancer before/after chemoradiation therapy using a MCA-microarray and bisulfate.

使用 MCA 微阵列和硫酸氢盐鉴定放化疗前后食管癌 DNA 甲基化变化。

• 发表时间: 2010
• 作者: Tsuda T;Watanabe Y;Itoh F
• 通讯作者: Itoh F