Relation of matrixmetalloproteinases and infalammatory bowel disease

基质金属蛋白酶与炎症性肠病的关系

• 批准号: 09670559
• 负责人: ITOH Fumio
• 金额: $ 2.3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09670559/
• 关键词: Matrilysin; Matrix metalloproteinase; RT-PCR; Immunostaining; ulcerative colitis; extracellular matrix; TIMP-1; TIMP-2; 潰瘍形成

Matrix metalloproteinase (MMP)-7, matrilysin, has been implicated in tumor invasion and metastasis as well as tumor initiation or growth. In previous reports, relation of matrix metalloproteinases and autoimmune diseases such as rheumatoid arthritis was investigated. However, matrix metalloproteinases in inflammatory bowel disease are not investigated in detail. In this study, several kinds of MMPs and tissue inhibitor of matrix metalloproteinases (TIMP) were immunohistochemically analyzed in, especially, ulcerative colitis. MMP-2, MMP-3, MMP-9, TIMP-1 and TIMP-2 were not remarkably up/down regulated in inflammatory region of ulcerative colitis using both biopsied specimens or surgically resected specimens. Interestingly, matrilysin (MMP-7) was detected in the epithelial cells near ulceration in severe ulcerative colitis. However, inflammatory region of mild to moderate epithelia did not show matrilysin expression. These results suggest that the role of matrilysin might be involved in repair process after inflammation in ulcerative colitis.

基质金属蛋白酶（MMP）-7，即基质溶解素，与肿瘤的侵袭和转移以及肿瘤的发生和生长有关。在以往的报道中，基质金属蛋白酶与自身免疫性疾病如类风湿性关节炎的关系进行了研究。然而，基质金属蛋白酶在炎症性肠病中没有详细研究。本研究对溃疡性结肠炎组织中的几种基质金属蛋白酶及其组织抑制剂（TIMP）进行了免疫组化分析。溃疡性结肠炎组织中MMP-2、MMP-3、MMP-9、TIMP-1和TIMP-2的表达在活检组织和手术切除组织中均未见明显上调或下调。有趣的是，基质溶解素（MMP-7）检测到溃疡附近的上皮细胞在严重的溃疡性结肠炎。而轻、中度上皮炎症区无基质溶解素表达。提示基质溶解素可能参与溃疡性结肠炎炎症后的修复过程。

项目成果

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Senota A、Itoh F、Imai K 等人：“Matrilysin 信使 RNA 表达与人胃癌侵袭活性的关系”Clin

Yamamoto H,Itoh F,Imai K,et al.: "Infrequent inactivation of DCC gene in replication error-positive colorectal cancers." Biochem Biophys Res Commun. (in press). (1998)

Yamamoto H、Itoh F、Imai K 等人：“复制错误阳性结直肠癌中 DCC 基因不频繁失活。”

Suzuki H, Itoh F, Imai K,: "Distinct methylation pattern and microsattelite instability in human gastric cancer"Int J Cancer,. 83. 303-309 (1999)

Suzuki H、Itoh F、Imai K，：“人类胃癌中的独特甲基化模式和微卫星不稳定性”Int J Cancer，。

MITA,H., ITOH,F., KIKUCHI,T., KAKIUCHI,H., HINODA,Y., IMAI,K.: "Isolation of the Epstein-Barr Virus in scirrhous gastric cancer by efficicy-minitored representational difference analysis."Tumor Biol. in press. (2000)

MITA,H.、ITOH,F.、KIKUCHI,T.、KAKIUCHI,H.、HINODA,Y.、IMAI,K.：“通过效率控制的代表性差异分析分离硬质胃癌中的 Epstein-Barr 病毒。