New visualization technique of radiation effects on cancers

辐射对癌症影响的新可视化技术

• 批准号: 13670956
• 负责人: FUJII Hirotada
• 金额: $ 2.3万
• 依托单位: Sapporo Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13670956/
• 关键词: free radicals; non-invasive visualization; ESR; oxygen; oximetry

It would be very desirable to be able to measure directly the oxygen tension in tissues to assess their state and response to therapy. This would be especially desirable for planning and evaluating the radiation therapy for tumors and vascular insufficiencies.The wide variety of techniques for measuring oxygen tensions in biological systems have been reviewed, but one of these methods has been demonstrated for the clinical use. Several NMR techniques have been studied recently, especially the use of an oxygen-dependent proton hyperfine line in myoglobin and oxygen-dependent relaxation of 19F. However, these approaches have not been demonstrated to have sufficient sensitivity and applicability for the real clinical use.Electron paramagnetic resonance (EPR) oximetry is a technique which is based on the effect of molecular oxygen on the line width of EPR spectra of some paramagnetic materials.In this project we used glucose char as the paramagnetic materials and examined its following characterizations : accuracy, sensitivity, ease to use, toxicity, and stability in vivo. From our results, the glucose char appears to have the very good properties than other reported paramagnetic materials.The EPR oximetry using the glucose char will be promising method to measure oxygen tensions in vivo.

人们非常希望能够直接测量组织中的氧分压，以评估其状态和对治疗的反应。这对于计划和评估肿瘤和血管不足的放射治疗尤其可取。已经回顾了各种测量生物系统中氧分压的技术，但这些方法中的一种已被证明用于临床。最近已经研究了几种核磁共振技术，特别是在肌红蛋白中的氧依赖质子超精细线的使用和19F的氧依赖弛豫。电子顺磁共振血氧仪是一种基于分子氧对某些顺磁性材料EPR谱线宽度影响的技术。在本课题中，我们以葡萄糖为顺磁性材料，考察了其准确性、灵敏度、易用性、毒性和体内稳定性。从我们的结果来看，葡萄糖碳具有比其他已报道的顺磁性材料非常好的性质。使用葡萄糖碳的EPR血氧仪将是一种很有前途的方法来测量体内的氧分压。

项目成果

Fujii H., Berliner L.J., Yoshikawa: "In vivo fate of paramagnetic iron oxide during sepsis"Mag.Reson.Imaging. 20. 271-276 (2002)

Fujii H.、Berliner L.J.、Yoshikawa：“败血症期间顺磁性氧化铁的体内命运”Mag.Reson.Imaging。

Fujii, H. et al.: "In Vivo Fate of Superparamagnetic Iron Oxides During Sepsis"Magnetic Resonance Imaging. 20(印刷中). (2002)

Fujii, H. 等人：“脓毒症期间超顺磁性氧化铁的体内命运”磁共振成像 20（出版中）。

Fujii H.et al.: "Consequences of nitric oxide generation in epileptic-seizure rodent models as studied by in vivo EPR"Magn.Reson.Med.. 48. 1051-1056 (2002)

Fujii H.等人：“通过体内 EPR 研究的癫痫发作啮齿动物模型中一氧化氮生成的后果”Magn.Reson.Med.. 48. 1051-1056 (2002)

Fujii H.et al.: "In vivo fate of superparamagnetic iron oxides during sepsis"Magn.Reson.Imaging. 20. 271-276 (2002)

Fujii H.等人：“败血症期间超顺磁性氧化铁的体内命运”Magn.Reson.Imaging。