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Development of ESR・MRI Dual Imager for Visualization of Reactive Oxygens

开发用于活性氧可视化的 ESR/MRI 双成像仪

基本信息

批准号：
15390363
负责人：
FUJII Hirotada
金额：
$ 8.38万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2004
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15390363/
关键词：
visualization MRI ESR active oxygen free radical superoxide 活性酵素画像化

项目摘要

Electron Paramagnetic Resonance Imaging (EPRI) technique has been developed to obtain the spatial distribution of the paramagnetic materials, particularly on a microscopic scale. A large number of biological studies were successfully carried out, especially for the visualization of bio-radicals generated in in vivo system. Typical example is the detection and visualization of nitric oxide (NO) in septic-shocked animals. The strength of EPRI is that only free radicals are visible in its image. On the contrary, weakness of EPRI is that nothing can be visible except paramagnetic materials on its image. Therefore, it seems very hard to obtain in which organs the bioradicals were generated in the small animals. In order to overcome this problem, there are three possible methods to visualize free radicals on the anatomical map of the object. 1: PEDRI (Proton Electron Double Resonance Imaging), 2: conventional MRI, and 3: EPR+MRI system. In this study, we have tried developing EPR/MRI dual imaging system to visualize free radicals on the anatomical map of the object.EPR/MRI dual imaging system was constructed by using the permanent magnet (Nb-Fe-B material) rather than electromagnets or superconductive magnets because it is easier to assemble and combine EPR and MRI components. EPR spectrometer in this dual imaging system is working at 1.2 GHz at 0.04T, and MRI spectrometer in this dual system is working at 22 MHz at 0.5T. The most surprising feature is that the center of MRI magnet is only 20 cm apart from that of EPR magnet. Each spectrometer is working well without any magnetic or electric interference.

电子顺磁共振成像(EPRI)技术的发展是为了获得顺磁材料的空间分布，特别是在微观尺度上。人们成功地开展了大量的生物学研究，特别是对体内生物自由基的可视化研究。典型的例子是败血症休克动物体内一氧化氮(NO)的检测和可视化。EPRI的强大之处在于它的图像中只有自由基可见。相反，EPRI的弱点是它的图像上除了顺磁材料之外什么都看不见。因此，似乎很难获得这些生物药物是在小动物的哪些器官中产生的。为了克服这个问题，有三种可能的方法在物体的解剖地图上可视化自由基。1：质子电子双共振成像(Pedri)，2：常规MRI，3：EPR+MRI系统。在这项研究中，我们尝试开发EPR/MRI双重成像系统来在物体的解剖地图上显示自由基。EPR/MRI双重成像系统是使用永磁体(Nb-Fe-B材料)而不是电磁铁或超导磁体来构建的，因为EPR和MRI组件更容易组装和结合。该双成像系统中的EPR光谱仪的工作频率为1.2 GHz，温度为0.04T，磁共振成像光谱仪的工作频率为22 MHz，温度为0.5T。最令人惊讶的是，MRI磁铁的中心距离EPR磁铁只有20厘米。每个光谱仪在没有任何电磁或电干扰的情况下工作正常。