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Molecular magnetic resonance imaging studies on ESR・MRI dual system

ESR・MRI双系统分子磁共振成像研究

基本信息

批准号：
17390335
负责人：
FUJII Hirotada
金额：
$ 9.92万
依托单位：
Sapporo Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-17390335/
关键词：
visualization ESR MRI cell protein 細胞・組織蛋白質 ESRI

项目摘要

For molecular imaging studies in the fields of biological and biomedical sciences, electron spin resonance (ESR)/magnetic resonance imaging (MRI) dual-imaging system was developed. This system, ESR/MRI dual-imaging system, was constructed using the permanent magnet (Nb-Fe-B material). The ESR and MRI part of the dual-imaging system was operated at 1.2 GHz (0.04 Tesla) and 22 MHz (0.5 Tesla), respectively. In order to obtain higher spatial resolution for ESR imaging of relatively-large line width, 2 mTesla/cm magnetic field gradient was used. We also developed ESR and MRI resonator for co-registration of ESR and MR images by using multi-coil parallel-gap resonator technique.We carried out two projects on our developed ESR/RI dual-imaging system.Project 1 : visualization and characterization of calcium influx in the rodent brain using paramagnetic manganese ions. In this project, we clarified that NMDA receptors play an important role for calcium influx when the neurons were activated.Project 2 : Measurement of brain tissue oxygenation under oxidative stress by ESR/MRI dual system. We have successfully monitored the change in p02 levels in vivo by ESR/MRI dual system with oxygen-sensing probe for relatively long terms. This system allowed us to measure pO_2 and nitric oxide generation simultaneously.

针对生物医学领域的分子成像研究，研制了电子自旋共振（ESR）/磁共振成像（MRI）双成像系统。该系统采用永磁体（Nb-Fe-B材料）构建，即ESR/MRI双重成像系统。双成像系统的ESR和MRI部分分别在1.2 GHz（0.04特斯拉）和22 MHz（0.5特斯拉）下运行。为了获得相对大线宽的ESR成像的更高的空间分辨率，使用2 mTesla/cm的磁场梯度。我们还利用多线圈平行间隙共振器技术研制了ESR和MRI共振器，用于ESR和MRI图像的配准。我们在我们研制的ESR/RI双成像系统上进行了两个项目：项目1：利用顺磁锰离子对啮齿动物脑内钙离子内流的可视化和表征。本课题阐明了NMDA受体在神经元激活时钙离子内流中的重要作用。课题2：ESR/MRI双系统检测氧化应激状态下脑组织氧合。我们已经成功地通过ESR/MRI双系统与氧传感探针在体内监测p02水平的变化相对较长的时间。该系统使我们能够同时测量pO_2和一氧化氮的产生。