Detection of Helicobacter sp. in bile and gallbladder tissue in patients with biliary tract diseases.

为探讨幽门螺杆菌感染在胆道癌发病机制中的作用，对2001年6月至2002年8月收治的66例胆道手术患者进行了幽门螺杆菌感染的调查，采用聚合酶链式反应(PCR16S RRNA)和套式聚合酶链式反应(NSTR860bpDNA)方法检测胆道组织和胆汁中的幽门螺杆菌特异性基因组DNA。仅1例胆囊癌组织16S rRNA阳性，12例(18.2%)检测到幽门螺杆菌特异的860bpDNA片段。而胆汁和胆汁培养的幽门螺杆菌均为阴性。组织学检查仅在1例胆囊癌标本中检出幽门螺杆菌-4ikerod、BRM微生物。结论：尽管在18%的患者中检测到幽门螺杆菌的DNA片段(可能是幽门螺杆菌)，但我们的结果并不支持在众所周知的胆道癌高发区新泻患者中胆道幽门螺杆菌感染显著高发的假设。然而，东京最近的一份报告描述了日本和泰国胆道疾病患者胆汁中16S rRNA的发病率令人惊讶地高。利用H.bilis特异性培养方法和H.bilis特异性聚合酶链式反应体系重建胆汁螺杆菌的研究方案是非常重要的。

To clarify the role of helicobacter infection in the pathogenesis of biliary tract cancer, evidence of helicobacter infection in patients who had cholecys^ectomy for the treatment of biliary tract diseases was investigated.Sixty-six patients who had biliary surgery between June 2001 and August 2002 were studied.Using removed gallbladder tissue and bile, Helicobacter specific genomic DNA was examined by Polymerase Chain Reaction (PCR) for 16S rRNA and by nested PCR for 860 bp genomic DNA fragment. 16S rRNA was positive in only one gallbladder tissue and helicobacter pylori specific 860 bp DNA fragment was detected hi 12 patients (18.2%). However, culture of gallbladder tissue and bile for helicobacter was negative in all. Histological examination revealed Helicobacter-4ikerod^brm microorganism in only one gallbladder specimen.Conclusion: Even though DNA fragment of helicobacer (probably of H.pylori) was detected in 18 % of the patients, our results did not support the presumption that we have significantly high prevalence of biliary tract helicobacter infection among patients of Niigata district where was well known as the high risk area for biliary tract cancer. However, a recent report from Tokyo described the surprisingly high incidence of helicobacter bilis specific 16S rRNA in bile of Japanese and Thai patients with biliary diseases. It was considered important to reconstruct research program focusing on H.bilis using H.bilis specific culture method and H.bilis specific PCR system.