Detection of Helicobacter sp. in bile and gallbladder tissue in patients with biliary tract diseases.

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To clarify the role of helicobacter infection in the pathogenesis of biliary tract cancer, evidence of helicobacter infection in patients who had cholecys^ectomy for the treatment of biliary tract diseases was investigated.Sixty-six patients who had biliary surgery between June 2001 and August 2002 were studied.Using removed gallbladder tissue and bile, Helicobacter specific genomic DNA was examined by Polymerase Chain Reaction (PCR) for 16S rRNA and by nested PCR for 860 bp genomic DNA fragment. 16S rRNA was positive in only one gallbladder tissue and helicobacter pylori specific 860 bp DNA fragment was detected hi 12 patients (18.2%). However, culture of gallbladder tissue and bile for helicobacter was negative in all. Histological examination revealed Helicobacter-4ikerod^brm microorganism in only one gallbladder specimen.Conclusion: Even though DNA fragment of helicobacer (probably of H.pylori) was detected in 18 % of the patients, our results did not support the presumption that we have significantly high prevalence of biliary tract helicobacter infection among patients of Niigata district where was well known as the high risk area for biliary tract cancer. However, a recent report from Tokyo described the surprisingly high incidence of helicobacter bilis specific 16S rRNA in bile of Japanese and Thai patients with biliary diseases. It was considered important to reconstruct research program focusing on H.bilis using H.bilis specific culture method and H.bilis specific PCR system.