Molecular analysis for chromatin structure conserved beyond generations

世代保守的染色质结构的分子分析

• 批准号: 22870038
• 负责人: YOSHIDA Keisuke
• 金额: $ 2.01万
• 依托单位: The Institute of Physical and Chemical Research
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Research Activity Start-up
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-22870038/
• 关键词: 次世代遺伝効果; 自然免疫系; マクロファージ; ATF-7; 自然免疫; ATF7

ATF-7 is reported to contribute to transgenerational effect of heat shock stress in fly. This time, I investigated molecular function of ATF-7 in innate immunity system using resident peritoneal macrophages. ChIP-chip results with ATF-7 antibody showed ATF-7 binds to promoter region of several tens of innate immunityrelated genes. At these sites, H3K9me2 level decrease and gene activity is enhanced in ATF-7 KO. I found that after treating macrophages by innate immune stress, ATF-7 binding and H3K9me2 level is decreased.

据报道，ATF-7有助于果蝇热休克应激的跨代效应。这次，我研究了ATF-7在先天免疫系统中的分子功能，使用驻留的腹腔巨噬细胞。ATF-7抗体的ChIP芯片结果显示ATF-7与数十个先天免疫相关基因的启动子区结合。在这些位点，ATF-7 KO中H3 K9 me 2水平降低，基因活性增强。我发现在通过先天免疫应激处理巨噬细胞后，ATF-7结合和H3 K9 me 2水平降低。

项目成果

ATF-7は炎症の抑制因子として機能する

ATF-7 具有炎症抑制剂的作用

• 发表时间: 2011
• 作者: 中山北斗;木村成介;吉田圭介
• 通讯作者: 吉田圭介