The effect of NO synthetase to vasodilation by anesthetic drag on model of pulmonary hypertension

目的：探讨桡动脉介入治疗的有效性。人桡动脉取自行冠状动脉旁路移植术的患者。准备好血管，将3 mm环悬挂在20 ml器官室中的金属丝上，器官室中充满缓冲盐溶液，维持在37 ℃，并用95% O2：5% CO2的混合物通气。其中一根金属丝固定在器官腔中，并连接到力传感器。将制备物反复暴露于60 mM KCl以在每个拉伸水平诱导收缩，直到获得最大收缩反应。我们决定静息张力为4g。通过环对10 μ M乙酰胆碱的反应来评估内皮功能<-5>。内皮功能几乎不残留。通过<-9><-4><-9><-4>与普萘洛尔（10 μ M）预孵育后去甲肾上腺素收缩的环的张力测量，获得奥普利农（10 μ M至10 μ M）或尼可地尔（10 μ M至10 μ M）的累积浓度-反应曲线。奥普力农或尼可地尔的血管舒张反应表示为去甲肾上腺素诱导的初始收缩的百分比，并且是剂量依赖性舒张剂。尼可地尔对5-羟色胺、U46619和苯丙氨酸氨基转移酶的舒张量效曲线呈剂量依赖性。最近，在格列本脲（10 μ M）或L-NAME（300 μ M）存在下，对尼可地尔的舒张量效曲线进行了研究。

Our purpose is to investigate the effectiveness of radial artery. Human radial artery was obtained from patients undergoing coronary artery bypass grafting. The vessels were prepared and 3 mm rings suspended on wires in 20 ml organ chambers filled with buffered salt solution maintained at 37 ℃ and aerated with a mixture of 95% O2 : 5% CO2. One of the wires was anchored in the organ chamber and the her was connected to a force transducer. The preparations were repeatedly exposed to 60 mM of KCl to induce contraction at each level of stretching, until a maximal contractile response was obtained. We decided resting tension to 4g. The endothelial function was assessed by the rings to 10^<-5> M of acetylcholine. The endothelial function was not almost residural. Cumulative concentration-response curves for olprinone (10^<-9> up to 10^<-4>M) or nicorandil (10^<-9>up to 10^<-4>M) were obtained from tension measurements of rings contracted by norepinephrine after a pre incubation with propranolol (10 μ M). The vasodilator responses of olprinone or nicorandil were expressed as a percentage of the initial contraction induced by norepinephrine, and were dose dependent relaxant. Dose response curves to serotonin, U46619 or phenylephrine were obtained dose dependent relaxant by nicorandil, Relaxation dose response curve to nicorandil is examing in the presence of glibenclamide (10 μ M) or L-NAME (300 μ M) recently.