The effect of NO synthetase to vasodilation by anesthetic drag on model of pulmonary hypertension

NO合成酶对肺动脉高压模型麻醉药物血管舒张的影响

• 批准号: 13671583
• 负责人: KURODA Masahiko
• 金额: $ 1.86万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671583/
• 关键词: radial artery; ring; nicorandil; olprinone

Our purpose is to investigate the effectiveness of radial artery. Human radial artery was obtained from patients undergoing coronary artery bypass grafting. The vessels were prepared and 3 mm rings suspended on wires in 20 ml organ chambers filled with buffered salt solution maintained at 37 ℃ and aerated with a mixture of 95% O2 : 5% CO2. One of the wires was anchored in the organ chamber and the her was connected to a force transducer. The preparations were repeatedly exposed to 60 mM of KCl to induce contraction at each level of stretching, until a maximal contractile response was obtained. We decided resting tension to 4g. The endothelial function was assessed by the rings to 10^<-5> M of acetylcholine. The endothelial function was not almost residural. Cumulative concentration-response curves for olprinone (10^<-9> up to 10^<-4>M) or nicorandil (10^<-9>up to 10^<-4>M) were obtained from tension measurements of rings contracted by norepinephrine after a pre incubation with propranolol (10 μ M). The vasodilator responses of olprinone or nicorandil were expressed as a percentage of the initial contraction induced by norepinephrine, and were dose dependent relaxant. Dose response curves to serotonin, U46619 or phenylephrine were obtained dose dependent relaxant by nicorandil, Relaxation dose response curve to nicorandil is examing in the presence of glibenclamide (10 μ M) or L-NAME (300 μ M) recently.

我们的目的是探讨桡动脉的有效性。桡动脉取自冠状动脉搭桥术患者。制备血管，将3mm环悬吊在钢丝上，置于20 ml充满缓冲盐溶液的器官腔中，保持在37℃，并以95% O2: 5% CO2的混合物曝气。其中一根电线固定在风琴腔中，并与力传感器相连。将这些材料反复暴露于60mm的氯化钾中，以在每个拉伸水平诱导收缩，直到获得最大收缩反应。我们将静息张力定为4g。以10^<-5> M的乙酰胆碱环评价内皮功能。内皮功能几乎不剩。在与普萘洛尔（10 μ M）预培养后，通过测量去甲肾上腺素收缩的环的张力，获得了olprinone （10^<-9> ~ 10^<-4>M）或nicorandil （10^<-9> ~ 10^<-4>M）的累积浓度-响应曲线。olprinone或nicorandil的血管扩张反应以去甲肾上腺素引起的初始收缩的百分比表示，并且是剂量依赖性松弛。获得了尼可地尔对5 -羟色胺、U46619和苯肾上腺素的剂量依赖性松弛剂的剂量反应曲线，最近在格列本脲（10 μ M）和L-NAME （300 μ M）的存在下研究了尼可地尔对松弛剂的剂量反应曲线。