Development of novel diagnosis and therapy against human oncogene hWAPL

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Human WAPL (hWAPL) gene is human homologue of wings apart-like (wapl) gene which encodes a protein that regulates heterochromatin structure of Drosophilia. Our previous study demonstrated that the novel human gene hWAPL showed the unscheduled high level expression in cervical dysplasia and carcinoma. In addition, NIH3T3 cells overexpressing hWAPL developed into tumours upon injection into nude mice, suggesting that hWAPL plays a significant role in cervical carcinogenesis and tumour progression as an oncogene. Because the repression of hWAPL by RNA interference (RNAi) using small interfering RNA (siRNA) decrease the tumor growth, the possibility that it becomes the target of the molecular target therapy is assumed. We identified the microRNA which regulated the expression of hWAPL gene this time. These microRNAs bound to 3'UTR of hWAPL gene and were shown to participate in repression of hWAPL mRNA and protein. We push forward analysis more and will investigate usefulness of the treatment of uterine cervical cancer with microRNA in future.