CHANGES IN CEREBROSPINAL MLCROC1RCULATION DURING AND AFTER ABDOMINAL AORTIC CROSS-CLAMPIN : AN UNDERLYING MECHANISM

腹主动脉交叉钳夹期间和之后脑脊髓 MLCR 的变化：潜在机制

• 批准号: 13671570
• 负责人: IIDA Hiroki
• 金额: $ 2.11万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671570/
• 关键词: Aortic cross-clamping; Cerebral vessel; Mechanism; Thromboxane A_2; Free radical; Milrinone; Colforsin daropate; Prostaglandin E_1; トロンボキサンA2; コルフルシンダルパート; プロスタグランジンE1; 脳; 脊髄; 微小循環; トロンボキサンA2受容体; 大動脈遮断; 解除

Pentobarbital anesthetized rabbits were prepared for measurement of cerebral pial vessel diameter in a cranial window preparation. We investigated the experiments according to following protocols.(1)We studied the effect of aortic clamping and declamping on cerebral pial vessel diameter. Although infrarenal aortic cross-clamping did not affect pial vessel diameter, release of a 20-min aortic cross-clamp caused pial arterioles to dilate and then constrict. A significant constriction persisted for at least 60 mm.(2)To clarify the mechanism of changes in cerebral microcirculation during and after abdominal aortic cross-clamping, we examined whether seratorodast, a thromboxane (Tx) A2 receptor antagonist, or edaravone, a free radical scavenger, would affect the respose observed after abdominal aortic cross-clamping. Both seratrodast and edaravone significantly attenuated the constriction of pial arterioles. Thus such vasoconstriction is partly induced via a washout of the TxA2 and free radicals produced in the ischemic region during and after cross-clamp release.(3)We investigated the effect of vasoactive agents such as milrinone, colforsin daropate, or prostaglandin E1 on arteriolar constriction observed after abdominal aortic cross-clamping. Clinical dose of milrinone and colforsin daropate but not prostaglandin El attenuated the constriction of pial arterioles after cross-clamp release.

戊巴比妥麻醉家兔，在颅窗准备中测量脑软膜血管直径。我们根据以下方案研究实验。(1)We研究主动脉阻断和开放对软脑膜血管直径的影响。虽然肾下主动脉阻断不影响软脑膜血管直径，但释放20分钟主动脉阻断导致软脑膜小动脉扩张，然后收缩。明显的收缩持续至少60 mm。(2)To为了阐明腹主动脉阻断期间和阻断后脑微循环变化的机制，我们研究了血栓素（Tx）A2受体拮抗剂塞拉司特或自由基清除剂依达拉奉是否会影响腹主动脉阻断后观察到的反应。塞曲司特和依达拉奉均显著减弱软膜小动脉的收缩。因此，这种血管收缩部分地通过在交叉夹释放期间和之后在缺血区域中产生的TxA2和自由基的洗脱而诱导。(3)We研究了血管活性剂如米力农、达罗酯或前列腺素E1对腹主动脉阻断后观察到的小动脉收缩的影响。临床剂量的米力农和colforsin daropate，但不是前列腺素El衰减后，交叉钳释放软脑膜小动脉的收缩。

项目成果

Ohata M et al.: "The effects of topical and intravenous ketamine on cerebral arterioles in dogs receiving pentobarbital or isoflurane anesthesia"Anesth Analg. 93. 697-702 (2001)

Ohata M 等人：“局部和静脉注射氯胺酮对接受戊巴比妥或异氟醚麻醉的狗脑动脉的影响”Anesth Analg。

Uchida M et al.: "Changes in cerebral microcirculation during and after abdominal aortic cross-clamping in rabbits : The role of thromboxane A2 receptor."Anesth Analg. 96. 651-656 (2003)

Uchida M 等人：“兔子腹主动脉交叉钳夹期间和之后脑微循环的变化：血栓素 A2 受体的作用。”Anesth Analg。

Iida H et al.: "The differential effects of stereoisomers of ropivacaine and bupivacaine on cerebral pial arterioles in dogs."Anesth Analg. 93. 1552-1556 (2001)

Iida H 等人：“罗哌卡因和布比卡因立体异构体对狗脑软膜小动脉的不同影响。”Anesth Analg。

Ohata H, Iida H, Nagase K, Dohi S: "The effects of topical and intravenous ketamone on cerebral arterioles in dogs receiving pentobarbital or isoflurane anesthesia"Anesth Analg. 93. 697-702 (2001)

Ohata H、Iida H、Nagase K、Dohi S：“局部和静脉注射酮对接受戊巴比妥或异氟烷麻醉的狗脑小动脉的影响”Anesth Analg。

Kiyoshi Nagase et al.: "Kefamine, Not Propofol, Attenuates Cerebrouascular Response to Carbon Dioxide in Humans With Isoflurane Anesthesia"J Clin Anesth. 13. 551-555 (2001)

Kiyoshi Nagase 等人：“异氟烷麻醉下，凯法明（而非异丙酚）可减弱人体对二氧化碳的脑血管反应”J Clin Anesth。