Interaction between ovarian carcinoma cells and mesothelial cells in peritoneal dissemination

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1.The expression of dipeptidyl peptidase IV (CD26) was confirmed in endometrial cancer tissues by immunohistochemical staining. The staining intensity decreased according to undifferentiation.2.The DPPIV cDNA was transfected into ovarian cancer cells (SKOV3). Growth rate did not changed in transfected cells (SKDPIV) compared to SKOV3 or vector transfected cells (SKpcDNA). However, migration and invasion potential significantly decreased in SKDPIV cells. Adhesion rate to mesothelial cells was significantly enhanced in SKDPIV cells, but not in SKpcDNA cells. Furthermore, DPPIV transfection induced morphological change from spindle to cobblestone like shape, while morphology of SKpcDNA cells were same as SKOV3 cells with spindle shape morphology.3.IL-1b, TNFa, and VEGE, which are much in malignant ascites, could increase DPPIV expression in mesothelial cells in a dose-dependent manner, while TGFb1 reduced expression of DPPIV.4.Transfection of DPPIV into SKOV3 cells enhanced E-cadherin and b-catenin expression. Furthermore, increased MMP-2 and MT1-MMP were observed in SKDPIV cells, while TLMIP-1 and -2 were decreased.5.We inoculated these 3 types of cells into nude mice. 30 days after inoculation, a number of disseminated tumors were observed in nude mice inoculated with SKOV3 or SKpcDNA cells, but no clear dissemination was observed in nude mice inoculated with SKPCDNA cells. Finally, prolonged survival was observed in nude mice inoculated with SKDPIV cells.6.Stromal derived factor 1a (SDF-1a), one of the DPPIV substrates, could increase progression and invasion in SKOV3 cells, but not in SKDPIV cells.