BASIC RESEARCH OF THE GENE THERAPY FOR ENDOMETRIOSIS

子宫内膜异位症基因治疗的基础研究

• 批准号: 13671703
• 负责人: ITO Naoki
• 金额: $ 2.3万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671703/
• 关键词: endometriosis; growth factors; receptors; endocrine disruptors; clear cell carcinoma (6); 内分泌撹乱化学物質; 内分泌攪乱化学物質

Endometriosis is reported to be increasing in recent years, and also the relation of endometriosis and dioxin in the animal model of rhesus monkey reported. This project was undertaken to examine the biological aspect of endometriosis for the apply of gene therapy in the near future and make the in vitro model of endometriosis.In the primary culture of endometriosis tissues, various kinds of growth factors and their receptors were checked under the presence of endocrine disruptors (dioxin and bisphenol A). No significant factors were found and in vitro cultures were difficult to be established. Then effects of endocrine disruptors on the proliferation of ovarian cancer cell lines was examined. Two cell lines were used in this project, Caov-3 is adenocarcinoma and ES2 is clear cell carcinoma. Relation of endometriosis and clear cell carcinoma reported in many past papers. Caov-3 proliferated with TCDD, when incubated in the low FBS condition. ES2 proliferation was increased with dioxin inoculation even in normal serum condition. Each ovarian cancer cell line might have its own different responsiveness to dioxin.

子宫内膜异位症近年来有增加的趋势，同时也有关于猕猴子宫内膜异位症与二恶英的关系的报道。本研究旨在探讨子宫内膜异位症的生物学机制，为将来基因治疗的应用奠定基础，并建立子宫内膜异位症的体外模型，在原代培养的子宫内膜异位症组织中，检测内分泌干扰物（二恶英和双酚A）存在下各种生长因子及其受体的表达。未发现显著因素，且难以建立体外培养。然后检测内分泌干扰物对卵巢癌细胞系增殖的影响。本项目中使用了两种细胞系，Caov-3是腺癌，ES 2是透明细胞癌。子宫内膜异位症与透明细胞癌的关系已有文献报道。Caov-3在低胎牛血清条件下与TCDD孵育时增殖。即使在正常血清条件下，二恶英接种也能增加ES 2的增殖。每种卵巢癌细胞系对二恶英的反应可能各不相同。

项目成果

N.Ito, T.Tamaya: "Dioxin and endometriosis"Igaku no Ayumi. 204(13). 959-963 (2004)

N.Ito, T.Tamaya：“二恶英和子宫内膜异位症”Igaku no Ayumi。

S.Hirano, S.Takahashi, N.Ito, T.Tamaya.: "Clinical implication of insulin-like growth factors through the presence of their binding proteins and receptors expressed in gynecological cancers."Eur.J.Gynaec.Oncol.. 25(2). 187-191 (2004)

S.Hirano、S.Takahashi、N.Ito、T.Tamaya.：“胰岛素样生长因子通过其结合蛋白和在妇科癌症中表达的受体的存在而产生的临床意义。”Eur.J.Gynaec.Oncol..

伊藤直樹: "ダイオキシンと子宮内膜症"医学のあゆみ. 204・2. 959-963 (2003)

伊藤直树：“二恶英和子宫内膜异位症”医学史204・2（2003）。

S.Hirano: "Clinical implication of insulin-like growth factors through the presence of their binding proteins and receptors expressed in gynecological cancers"Eur.J.Gynaec.Oncol.. 25・2. 187-191 (2004)

S.Hirano：“妇科癌症中表达的胰岛素样生长因子的结合蛋白和受体的存在的临床意义”Eur.J.Gynaec.Oncol.. 25・2 (2004)。