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The effect of prolactin on arachidonic acid metabolism in amniotic cells

催乳素对羊膜细胞花生四烯酸代谢的影响

基本信息

批准号：
06671667
负责人：
MIYAKAWA Isao
金额：
$ 1.41万
依托单位：
Department of Obstetrics and Gynecology, Oita Medical University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1994
资助国家：
日本
起止时间：
1994 至 1995
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-06671667/
关键词：
Prolactin Prostaglandins Amniotic cells

项目摘要

Recent study have characterized and cloned two type of cyclooxygenase. Cyclooxygenase type 1 (COX-1) is constitutive form. On the other hand, cyclooxygenase type 2 (COX-2), a new form of cyclooxygenase, is expressed only after stimulation by various cytokines.Cells (amnion-derived WISH cells) were cultured and stimulated by phorbol myristate acetate (PMA). The PGE2 in culture media were measured by ELISA.Total RNA was extracted and analyzed with Northern blotting analysis.In the time course of PGE2 production in the presence or absence PMA,the concentration of PGE2 was not detected until 2h of incubation, but was detected after 4h. The stimulation and suppression of PGE2 production in the presence or absence PMA and/or DEX,PGE2 was significantly increased by PMA and suppressed by PMA and DEX,and DEX only. In the time course of COX-2 mRNA in the presence or absence PMA,the band of COX-2 mRNA was detected after 1h. The most strong band of COX-2 mRNA was detected at 2h. In the stimulation and suppression of COX-2 mRNA in the presence or absence PMA and/or DEX,the band of COX-2 mRNA stimulated by PMA was strongly detected and the band stimulated by DEX was not detected. These results suggested that COX-2 was present and expressd by activated protein kinase C.The COX-2 might be important role of labor and deliverly in parturition.

最近的研究已经鉴定和克隆了两种类型的环氧合酶。环氧合酶-1(COX-1)是一种结构型。另一方面，环氧合酶-2(COX-2)是环氧合酶的一种新形式，只有在各种细胞因子刺激下才能表达。用ELISA法检测培养基中PGE2的含量，提取总RNA进行Northern印迹分析，在有无PMA作用下的PGE2产生过程中，培养2小时才能检测到PGE2的浓度，4h后才能检测到PGE2的浓度。有或无PMA和/或DEX对PGE_2的刺激和抑制作用，PMA可显著促进PGE_2的产生，而PMA和DEX可抑制PGE_2的产生，而单独使用DEX可抑制PGE_2的产生。在PMA作用下COX-2mRNA表达的时程中，1h后检测到COX-2mRNA的条带。环氧合酶-2mRNA在2小时出现最强条带。在有或无PMA和/或DEX对COX-2mRNA的刺激和抑制中，PMA刺激的COX-2mRNA条带较强，而地塞米松刺激的条带不明显。提示COX-2在分娩过程中可能起重要作用，并通过活化蛋白激酶C表达。