A placenta-specific gene, polo like kinase, expression and its biological role

胎盘特异性基因、polo样激酶、表达及其生物学作用

• 批准号: 09671699
• 负责人: MIYAKAWA Isao
• 金额: $ 2.24万
• 依托单位: OITA MEDICAL UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1997
• 资助国家: 日本
• 起止时间: 1997 至 2000
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-09671699/
• 关键词: polo like kinase; placenta; trophoblast; decidua; endometrium; ovary; ovarian cancer; endometrial cancer

To clarify the mechanism involving placental development and functions, we have cloned a new placenta-specific kinase gene from a placental cDNA library and named Polo like kinase(PLK). The Northern blot analysis demonstrated the highest expression only in the placenta but not in other tissues including the brain, lung, liver and kidney [Hamanaka et al. Cell Growth ＆ Differ. 5 : 249-257, 1994].The immunohistochemical study of the placenta showed that human PLK was strongly expressed in the syncytiotrophoblast during the early stages of pregnancy [Yoshimatsu et al. Res.Commun.Mol.Pathol.Pharmacol. 106 : 3-12, 1999]. Murine PLK was strongly expressed in troploblastic tissue invading the decidua and in trophoblastic tissue around embryos [Takai et al. Reprod.Fertil.Dev. 11 : 31-35, 1999]. In normal human endometrium during the menstrual cycle, PLK is associated with estrogen-dependent cellular proliferation and estrogen functions may be involved in its regulation [Takai et al. Reprod.Fertil.Dev. 12 : 59-67, 2000]. Furthermore, PLK expression in ovarian cancer and endometrial cancer is associated with high histological grade [Takai et al. Cancer Lett. 164 : 41-49, 2001].

为了阐明胎盘发育和功能的机制，我们从胎盘cDNA文库中克隆了一个新的胎盘特异性激酶基因，命名为波罗样激酶（PLK）。北方印迹分析证明仅在胎盘中表达最高，而在其他组织中不表达，包括脑、肺、肝和肾[Hamanaka等人，Cell Growth & Differ.第五章：249-257，1994]。胎盘的免疫组织化学研究表明，在妊娠早期，人PLK在合体滋养层中强烈表达[Yoshimatsu等，Res.Commun.Mol.Pathol.Pharmacol. 106：3-12，1999]。鼠PLK在侵入蜕膜的滋养层组织和胚胎周围的滋养层组织中强烈表达[Takai等人，Reprod.Fertil.Dev. 11：31-35，1999]。在月经周期期间的正常人子宫内膜中，PLK与雌激素依赖性细胞增殖相关，并且雌激素功能可能参与其调节[Takai等人，Reprod.Fertil.Dev. 12：59-67，2000]。此外，卵巢癌和子宫内膜癌中的PLK表达与高组织学分级相关[Takai等人，Cancer Lett. 164：41-49，2001]。

项目成果

Noriyuki Takai, Tami Miyazaki, Isao Miyakawa, Ryoji Hamanaka: "Polo-like kinase (PLK) expression in normal human endometrium during the menstrual cycle."Reprod.Fertil.Dev.. 12 (1, 2). 59-67 (2000)

Noriyuki Takai、Tami Miyazaki、Isao Miyakawa、Ryoji Hamanaka：“月经周期期间正常人子宫内膜中 Polo 样激酶 (PLK) 的表达。”Reprod.Fertil.Dev.. 12 (1, 2)。

Tadamitsu Kishimoto: "Leukocyte Typing VI, white cell differentiation antigens" Garland Publishing,lnc., 1342 (1998)

Tadamitsu Kishimoto：“白细胞分型 VI，白细胞分化抗原” Garland Publishing,lnc.，1342 (1998)

Noriyuki Takai: "Polo-like kinase (PLK) expression in normal human endometrium during the menstrual cycle."Reprod.Fertil.Dev.. 12(1,2). 59-67 (2000)

Noriyuki Takai：“月经周期期间正常人子宫内膜中 Polo 样激酶 (PLK) 的表达。”Reprod.Fertil.Dev.. 12(1,2)。

Noriyuki Takai, Tami Miyazaki, Kayo Fujisawa, Kaei Nasu, Isao Miyakawa: "Expression of receptor tyrosine kinase EphB4 and its ligand ephrin-B2 is associated with malignant potential in endometrial cancer."Oncol.Rep.. (in press). (2001)

Noriyuki Takai、Tami Miyazaki、Kayo Fujisawa、Kaei Nasu、Isao Miyakawa：“受体酪氨酸激酶 EphB4 及其配体 ephrin-B2 的表达与子宫内膜癌的恶性潜能相关。”Oncol.Rep..（出版中）。

Hisashi Narahara: "Platelet-activating factor acetylhydrolase activity in human follicular fluid." Advances in Experimental Medicine and Biology. 416. 121-127 (1997)

Hisashi Narahara：“人卵泡液中的血小板激活因子乙酰水解酶活性。”