Correlation between optic nerve projection changes and neural functional plasticity, following to damage in anteriorvisual pathuazs.

前视觉路径损伤后视神经投射变化与神经功能可塑性之间的相关性。

• 批准号: 13671827
• 负责人: KIYOSAWA Motohiro
• 金额: $ 1.98万
• 依托单位: Tokyo Medical and Dental University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671827/
• 关键词: rat; optic nerve; optic chiasma; enucleation; Adenosine reaptovs; Benzodiazepne receptors; cerebral glucose metabolism; Autoradiogram; 糖代謝; 神経受容体密度; 神経投射; イメージングプレート; 眼瞼縫合; Adenosine A1; Bengodiagepine; neceptor; rat; brain; enucleation

Purpose : To determine the changes in the densities of adenosine A_1, and benzodiazepine receptors densities and in glucose metabolism in the visual centers of rat brain following monocular enucleation or eyelid suture on postnatal day 10 (PN10). Methods : Following monocular enucleation or eyelid suture on PN10 rats, the alterations in the adenosine A_1, and benzodiazepine receptor densities and in glucose metabolism were evaluated in superior colliculus (SC), dorsal lateral geniculate body (DLG), and visual cortex (VC) by ex vivo autoradiography with [^<11>C]MPDX, [^<11>C]flumazenil and[^<14>C]2-deoxyglucose, respectively. Results : Enucleation reduced the [^<11>C]MPDX binding in SC and DLG, and enhanced the [^<11>C]flumazenil binding in SC. Eyelid suture reduced the [^<11>C]flumazenil binding in VC at day 20. [^<14>C]2-deoxyglucose uptake was not decreased by enucleation in any region except in SC and DLG at day 1, but was decreased by eyelid suture in SC at day 20 and 55 and in VC at day 55. Conclusions : The decrease in the presynaptic adenosine A_1, receptors in SC and DLG following enucleation is coupled with an up-regulation of postsynaptic beuzodiazepine receptors. The development of neural architecture for visual functions is not completed at PN10 in rats. Neural reactions following enucleation are completely different from those following eyelid suture.

目的：目的：观察大鼠出生后10天（PN 10）单眼眼球摘除或眼睑缝合后视觉中枢腺苷A_1受体密度、苯二氮卓受体密度及葡萄糖代谢的变化。研究方法：应用离体放射自显影技术，分别用[^ C]MPDX、[^ C]氟马西尼和[^ C]2-脱氧葡萄糖，观察了PN 10大鼠单眼眼球摘除或眼睑缝合后，上级丘（SC）、背外侧膝状体（DLG）和视皮层（VC）腺苷A_1和苯二氮卓受体密度以及葡萄糖代谢的变化<11><11><14>。结果：去核减少了<11>SC和DLG中[^ C]MPDX结合，并增强了SC中[^<11>C]氟马西尼结合。眼睑缝合减少了VC中[^<11>C]氟马西尼结合。除<14>第1天的SC和DLG外，任何区域的[^ C]2-脱氧葡萄糖摄取均未因去核而降低，但在第20天和第55天的SC和第55天的VC中，眼睑缝合降低了[^ C]2-脱氧葡萄糖摄取。结论：去核后SC和DLG突触前腺苷A_1受体的减少与突触后beuzodiazepine受体的上调相耦合。大鼠视觉功能的神经结构发育在PN 10时尚未完成。眼球摘除术后的神经反应与眼睑缝合术后的神经反应完全不同。

项目成果

Wang WF, Ishiwata K, Kiyosawa M, et al.: "Receptors and metabolism in the visual stractures of rat monocularly deprivedata sensitive period"Jpn. J. Ophthalmol.. 47(in press). (2003)

Wang WF，Ishiwata K，Kiyosawa M，等：“单眼剥夺数据敏感期大鼠视觉结构中的受体和代谢”Jpn。

清澤源弘: "神経節疾患のとらえ方"文光堂. 80-91 (2001)

Minahiro Kiyosawa：“如何理解神经节疾病”Bunkodo 80-91（2001）。

Wang W-F, Ishiwata K, Kiyosawa M, Kawamura k, Oda K, Kobayashi T, Matsuno K, Mochizuki M.: "Visualization of sigmal receptors in eyes by ex vivo autoradiography and in vivo positron emission tomography"Experimental Eye Research. 75. 723-730 (2002)

Wang W-F、Ishiwata K、Kiyosawa M、Kawamura k、Oda K、Kobayashi T、Matsuno K、Mochizuki M.：“通过离体放射自显影和体内正电子发射断层扫描对眼睛中的 sigmal 受体进行可视化”实验眼研究。

Kiyosawa M, Ishiwata K, et al.: "Neuroreceptor bindings and synaptic activity in vusual system of monocularly enucleated rat"Jpn J Ophthalmol. 45. 264-269 (2001)

Kiyosawa M，Ishiwata K，等人：“单眼摘除大鼠视觉系统中的神经受体结合和突触活性”Jpn J Ophamol。

Wang WF, Ishiwata K, Kiyosawa M, et al.: "Visualization of signal receptors in eyes by exvivo autoradiography and in vivo positron emission tomography"Experimental Eye Research. 75. 723-730 (2002)

Wang WF、Ishiwata K、Kiyosawa M 等人：“通过离体放射自显影和体内正电子发射断层扫描对眼睛中的信号受体进行可视化”实验眼研究。