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Receptor density in monocularly deneruated rats

单眼失能大鼠的受体密度

基本信息

批准号：
11671726
负责人：
KIYOSAWA Motohiro
金额：
$ 2.24万
依托单位：
Tokyo Medical and Dental University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1999
资助国家：
日本
起止时间：
1999 至 2000
项目状态：
已结题

项目摘要

PURPOSE.To study the changes in adenosine A1 and benzodiazepine receptors and glucose metabolism in the visual structures of rats following monocular deprivation by enucleation and eyelid suture at postnatal day 10.METHODS.The right eye of rats were deprived by enucleation or eyelid suture and ex vivo autoradiography of the brain was performed at different times up to 45 days after the treatment. [1-methyl-1 1C]8-dicyclopropylmethyl-1-methyl-3-propylxanthine ([11C]MPDX) and [11C]flumazenil were used to map adenosine A1 receptors and benzodiazepine, respectively, and glucose metabolism was evaluated with [14C]2-deoxyglucose, and. The receptor-specific binding for 11C activity and the uptake of 14C activity in the superior colliculus (SC), visual cortex (VC) and dorsal lateral geniculate body (DLG) were determined.RESULTS.The specific binding of [11C]MPDX to the adenosine A1 receptors in the contralateral SC significantly decreased immediately after enucleation and remained low thereafte … More r, whereas the specific binding of [11C]flumazenil to benzodiazepine receptors in the contralateral SC increased after 5 days. The uptake of [14C]2-deoxyglucose in the SC was depressed only one day after enucleation and then returned to the ipsilateral level. No significant difference between contralateral and ipsilateral sides was found in any other visual structures at any time investigated for the all three tracers. On the other hand, after the eyelid suture all three tracers showed symmetrical images without any detectable changes.CONCLUSIONS.The axon degeneration related a decrease of the adenosine A1 receptor density resulted in an upregulation of postsynaptic benzodiazepine receptor density in monoculaely enucleated, not in eyelid sutured, rats at PN 10. Neither visual deprivation methods induced alteration in both receptors nor in glucose metabolism in any visual structures, except that the glucose metabolism was decreased only in the contralateral SC one day after the enucleation. These results suggest that the benzodiazepine receptors are susceptible to degeneration of presynaptic nerve terminals, but not to visual deprivation, and that the visual functions are not determined in rats at PN 10. Less

目的：研究出生后10 d单眼剥夺（眼球摘除+眼睑缝合）大鼠视觉结构腺苷A1受体和苯二氮卓受体的变化及葡萄糖代谢的变化。方法：采用眼球摘除或眼睑缝合法剥夺大鼠右眼，在治疗后45 d的不同时间进行离体脑放射自显影。[1-甲基-11 C]8-二环丙基甲基-1-甲基-3-丙基黄嘌呤（[11 C]MPDX）和[11 C]氟马西尼分别用于绘制腺苷A1受体和苯二氮卓类药物的图谱，并用[14 C]2-脱氧葡萄糖评价葡萄糖代谢。结果：去核后，[11 C] MPDX与对上级上丘（SC）腺苷A1受体的特异性结合迅速下降，并在去核后保持较低水平，[11 C]MPDX与对侧SC腺苷A1受体的特异性结合在去核后的第一个时间点显著下降，在去核后的第二个时间点显著下降。 ...更多信息而[11 C]氟马西尼与对侧SC中苯二氮卓类受体的特异性结合在5天后增加。[14 C]2-脱氧葡萄糖在SC中的摄取仅在去核后一天被抑制，然后恢复到同侧水平。对侧和同侧之间没有显着差异，发现在任何其他视觉结构在任何时间调查的所有三个示踪剂。另一方面，眼睑缝合后，所有三个示踪剂显示对称的图像没有任何可检测的changes.CONCLUSIONS.轴突变性相关的腺苷A1受体密度的减少导致突触后苯二氮卓类受体密度在monoculaely摘除，而不是在眼睑缝合，大鼠在PN 10的上调。无论是视觉剥夺的方法诱导改变受体，也不是在任何视觉结构中的葡萄糖代谢，除了葡萄糖代谢下降，只有在对侧SC一天后，去核。这些结果表明，苯二氮卓类受体对突触前神经末梢的变性敏感，但对视觉剥夺不敏感，并且在PN 10时大鼠的视觉功能未被确定。少