Could ganglion cell transplantation or ganglion tissue transplantation be a treatment of aganglionosis?

神经节细胞移植或神经节组织移植可以治疗神经节缺失症吗？

• 批准号: 13671873
• 负责人: TANAKA Yoshiaki
• 金额: $ 1.41万
• 依托单位: KURUME UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671873/
• 关键词: Ganglion cell transplantation; Ganglion tissue transplantation; Aganglionosis; 神経節移植; GFP; 遺伝子銃

To investigate whether the ganglion cell or the ganglion tissue transplantation could be an optimal treatment for aganglionosis, the following study was performed.AnimalsThe Lewis rats were used as donors and recipients in this experiment.Dorsal root ganglion tissue transplantation studyThe rat dorsal root ganglion tissue which was taken from donor rat was transplanted to subserosal space of colon in recipient rat.The recipient were divided into two groups.The normal group (n=10) was the group which the dorsal root ganglion tissue was transplanted to normal colon. The ischemia-reperfusion group (n=10) was the group which the dorsal root ganglion tissue was transplanted to colon with ischemia-reperfusion stress. The ischemia-reperfusion stress was made by clamping and unclamping of mesenteric arterial branch of colon.The recipient rats were sacrificed two weeks after transplantation and colon was excised. The samples were fixed with 10% formaldehyde and stained with hematoxylin-eosin stain, chromogranin stain, or synaptophysin stain.They were evaluated microscopically.The result of dorsal root ganglion tissue transplantation studyThe transplanted ganglion tissues were successfully adhered in the both two groups. In the normal group, the clear border between recipient tissue and donor ganglion tissue was present, while the border was unclear in the ischemia-reperfusion group.Dorsal root ganglion cell transplantation studyWe also performed the dorsal root ganglion cells transplantation. The dorsal root ganglion cells were obtained from rat and the cell transplantation to recipient colon was performed. They are now evaluating histologically. In addition, the dorsal root ganglion cell to which the PGFP gene is induced is now planning.

为了探讨神经节细胞或神经节组织移植是否是治疗无神经节细胞症的最佳方法，动物实验选用刘易斯大鼠作为供体和受体，背根神经节组织移植研究取供体大鼠背根神经节组织，移植到受体大鼠结肠浆膜下间隙，受体分为两组，每组10只。正常组（n=10）为将背根神经节组织移植到正常结肠的组。缺血再灌注组（n=10）为将背根神经节组织移植到结肠内，造成缺血再灌注应激。通过夹闭和松开结肠肠系膜动脉分支造成缺血再灌注应激，移植后2周处死受体大鼠，切除结肠。标本经10%甲醛固定，苏木精-伊红染色、嗜铬粒蛋白染色、突触素染色，光镜下观察。背根神经节组织移植研究结果两组移植神经节组织均成功粘附。正常组受区与供区神经节组织边界清晰，缺血再灌注组受区与供区神经节组织边界不清。背根神经节细胞移植研究我们同时进行了背根神经节细胞移植。取大鼠背根神经节细胞，移植于受体结肠。他们现在正在进行组织学评估。此外，PGFP基因被诱导的背根神经节细胞现在正在计划中。