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Analysis of species-specific function of neuropeptides in insects.

昆虫神经肽的物种特异性功能分析。

基本信息

批准号：
15580043
负责人：
TANAKA Yoshiaki
金额：
$ 2.3万
依托单位：
Notional Institute of Agrobiological Sciences (NIAS)
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2003
资助国家：
日本
起止时间：
2003 至 2005
项目状态：
已结题

项目摘要

1.Distribution of prothoracicostatic peptide (PTSP)-related peptides (PRPs) in the central nervous system (CNS) was investigated using the monoclonal antibodies against PTSP in several lepidopteran insects. In the brain and corpora-cardiaca, different molecules of PRPs seemed to be predominant between Bombyx mori and other lepidopteran insects. In the sweet potato hornworm, Agrius convoluvulli, PRPs seemed to be released from the epiproctodeal glands during molting period, but the timing of secretion from this gland was different among these insects. These results suggest that the distribution and secretion of PRP are different among the lepidopteran insects.2.The cDNA encoding the receptor for corazonin (Crz) was cloned from the CNS of B.mori. Quantitative real-time PCR analysis suggested that this receptor was predominantly expressed hi the epitracheal gland, anterior silkgland, and CNS, but not in the epidermis, although epidermis is thought to be a possible target tissue of corazonin in the locusta. This result suggests that the distribution of corazonin receptor is different among insect species, which causes the different physiological function. The Crz gene was cloned from brains of the honey bee workers (Apis mellifera). The ApimeCrz cDNA encodes a new form of corazonin displaying Thr^4 substitution of [His^7]-corazonin.3.New prothoracicostatic factors, Bombyx myosuppressin (BMS) and Bombyx FMRFamide (BRFa), were isolated and the cDNAs encoding these peptides cloned from the brains and prothoracic ganglion of B.mori, respectively. Direct mass spectrometory and electrophysiological analyses demonstrated that BRFa was supplied to the surface of the prothoracic gland (PG) via neurons innervating from the prothoracic ganglion during early to mid fifth instar. To our knowledge, this study provides the first report of peptides controlling ecdysteroidogenesis by direct innervation.

1.使用针对几种鳞翅目昆虫的PTSP单克隆抗体研究了中枢神经系统（CNS）中前胸腺抑制肽（PTSP）相关肽（PRP）的分布。在大脑和心体中，不同的 PRP 分子似乎在家蚕和其他鳞翅目昆虫之间占主导地位。在甘薯天蛾 Agrius convoluvulli 中，PRP 似乎在蜕皮期间从肛上腺释放，但这些昆虫从该腺分泌的时间不同。这些结果表明PRP在鳞翅目昆虫中的分布和分泌存在差异。2.从家蚕中枢神经系统克隆了编码corazonin受体(Crz)的cDNA。定量实时PCR分析表明，该受体主要在气管上腺、前丝腺和中枢神经系统中表达，但不在表皮中表达，尽管表皮被认为是蝗虫中corazonin的可能靶组织。这一结果表明，corazonin受体在昆虫物种之间的分布存在差异，从而导致其生理功能的不同。 Crz 基因是从工蜂 (Apis mellifera) 的大脑中克隆出来的。 ApimeCrz cDNA 编码一种新形式的 corazonin，显示 [His^7]-corazonin 的 Thr^4 取代。 3. 分离出新的前胸抑制因子，即家蚕肌抑制素 (BMS) 和家蚕 FMRFamide (BRFa)，并从家蚕的大脑和前胸神经节中克隆了编码这些肽的 cDNA。分别是B.mori。直接质谱和电生理学分析表明，在第五龄早期到中期，BRFa 通过前胸神经节支配的神经元供应到前胸腺 (PG) 表面。据我们所知，这项研究首次报告了肽通过直接神经支配控制蜕皮类固醇生成。

项目成果

期刊论文数量（31）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Comparison structure-activity relations of corazonin using two different bioassay systems

使用两种不同生物测定系统比较 corazonin 的构效关系

DOI：
发表时间：
2003
期刊：
Peptides 24
影响因子：
0
作者：
Ozawa;T.;Risal;C.P.;Yanagimoto;R.;Soga;Y.;Roller L. et al.;Yamanaka N. et al.;Roller L. et al.;Yamanaka N. et al.;Roller L. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Liu X.et al.;Tanaka Y. et al.
通讯作者：
Tanaka Y. et al.

FMRFamide 相关肽调节前胸腺活动。

DOI：
发表时间：
2005
期刊：
Pestycydy 3
影响因子：
0
作者：
Ozawa;T.;Risal;C.P.;Yanagimoto;R.;Soga;Y.;Roller L. et al.;Yamanaka N. et al.;Roller L. et al.;Yamanaka N. et al.;Roller L. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Yamanaka N. et al.;Yamanaka N. et al.
通讯作者：
Yamanaka N. et al.

Roller L et al.: "Corazonin and corazonin-like substances in the central nervous system of the Pterygote and Apterygote insects."Cell and Tissue Research. 312. 393-406 (2003)

Roller L 等人：“翼状和无翼昆虫中枢神经系统中的 Corazonin 和 Corazonin 样物质。”细胞和组织研究。

DOI：
发表时间：
期刊：
影响因子：
0
作者：
通讯作者：

Regulation of insect steroid hormone biosynthesis by innervating peptidergic neurons.

DOI：
10.1073/pnas.0511196103
发表时间：
2006-06
期刊：
Proceedings of the National Academy of Sciences of the United States of America
影响因子：
11.1
作者：
N. Yamanaka;D. Z̆itn̆an;Young-Joon Kim;M. Adams;Y. Hua;Yusuke Suzuki;Minoru Suzuki;A. Suzuki;H. Satake;A. Mizoguchi;K. Asaoka;Yoshiaki Tanaka;H. Kataoka
通讯作者：
N. Yamanaka;D. Z̆itn̆an;Young-Joon Kim;M. Adams;Y. Hua;Yusuke Suzuki;Minoru Suzuki;A. Suzuki;H. Satake;A. Mizoguchi;K. Asaoka;Yoshiaki Tanaka;H. Kataoka

Molecular cloning of [Thr^4 His^7]-corazonin (Apime-corazonin) and its distribution in the central nervous system of the honev bee Apis mellifera (Hymenoptera : Apidae.

[Thr^4 His^7]-corazonin (Apime-corazonin) 的分子克隆及其在蜜蜂中枢神经系统中的分布（膜翅目：蜜蜂科。