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Chemical analysis and immunohistology research on non-collagen protein that takes part in the formation of calcospherites

参与钙球体形成的非胶原蛋白的化学分析和免疫组织学研究

基本信息

批准号：
13671914
负责人：
MISHIMA Hiroyuki
金额：
$ 1.98万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671914/
关键词：
Globular calcification Linear calcification Calcospherite Orientation of apatite crystal Collagen fibers Mode of calcification Crown dentin Root deuntin プロテオグリカン成長線

项目摘要

On the mammal, the mineralization of mantle dentin is formed with the mineralization of the matrix vesicle. But the mineralization of circumpulpal dentin is formed by the mineralization that relates to collagen fibers. The spherical calcospherites were observed in the predentin of crown dentin. In the apical region of root dentin, the calcospherites were not observed and the calcified collagen fibers were observed. The species specificity was detected in the development and distribution of calcospherites. As compared with in the cementum covered lingual dentin of continuously growing teeth such as rat and rabbit incisors, and in the cementum covered root dentin uncontinuously growing teeth such as humans and bovine incisors, the form and the size of calcospherites were greatly different. In the marsupial opossum dentin, the calcospherites were only observed at the cervical region of crown. In the reptile crocodilian dentin, the size of the calcospherites was smaller than that of mammal … More , and the fiber-like structure was observed on the surface of calcospherites. On the Ca/P ratio of calcospherites in human, bovine and opossum, the significant difference had been found between the crown and the root of dentin. The difference was not found on the Ca/P ratio of calcospherites in the continuously growing teeth such as rat and rabbit, and polyphyodont crocodilian. It is considered that the difference of the mechanisms of teeth formation related to that of calcospherites formation. With the results of the analytical electron microscopy, except the main element of calcium and phosphorus, elements of Mg S, K, and Al were detected in the stage of calcospherite formation. It was clarified that Mg especially had the key role the formation of the calcospherites among small amount elements from the observation of the analysis electron microscope A lot of Mg was detected in the first stage of the formation of calcospherites. Mg may contribute to physicochemical stability of the apatite crystal. It seems that Mg takes part in the nuclear formation on the initial stage of calcospherites formation. Moreover, it is considered that the localization of S means the existence of the proteoglycan that is non-collagen protein in the calcospherites. It is judged that S of the sulfuric acid radical in the proteoglycan was detected. The calcospherites of crocodilian were small and S was distributed from the cervical region to the root region of dentin. It was considered that the proteoglycan controlled the formation of calcospherites because it took part in the formation of collagen fibers and has closer affinity with Ca. When a human molar was stained with the antiheparan sulfate antibody, the immune reaction attitude was different between the crown pulp and the root pulp. It is considered that this opinion is related to the difference in the form and development of calcospherites between the crown and the root dentin. The reaction was seen well in the region with which dentin was being firmed in the predentin of crown. The calcospherites existed in the mineralization front of dentin, and it was suggested that the form of calcospherites was related to the localization of the heparan sulfate. Less

哺乳动物的牙本质矿化是随着基质囊泡矿化而形成的。而牙髓周围牙本质的矿化是由与胶原纤维有关的矿化形成的。在冠状牙本质前牙本质中观察到球状钙球粒。根牙本质顶端未见钙球粒形成，可见钙化的胶原纤维。在钙球石的发育和分布中发现了物种特异性。与连续生长的牙骨质覆盖舌牙本质（如大鼠、家兔）和非连续生长的牙骨质覆盖根牙本质（如人类、牛门牙）相比，钙球石的形态和大小有很大差异。在负鼠牙本质中，钙球粒仅在冠颈区可见。爬行动物的牙本质中，钙球粒的大小比哺乳动物的小，在钙球粒表面可见纤维状结构。在人、牛和负鼠钙球石的钙磷比上，牙本质的冠与根之间存在显著差异。大鼠、家兔等连续生长牙齿和多齿鳄的钙球石钙磷比无显著差异。认为牙齿形成机制的差异与钙球石形成机制的差异有关。分析电镜结果显示，钙球石形成阶段除主要元素钙、磷外，还检出镁、硫、钾、铝等元素。通过分析电镜观察，明确了微量元素中Mg对钙球石的形成起着关键作用，在钙球石形成的第一阶段就检测到大量的Mg。镁可能有助于磷灰石晶体的物理化学稳定性。镁元素似乎在钙球石形成初期参与了核的形成。此外，认为S的定位意味着钙球粒中存在非胶原蛋白的蛋白多糖。判断该蛋白多糖中检测到硫酸自由基S。鳄鱼的钙球粒小，S分布于牙本质的颈区至根区。认为蛋白多糖参与胶原纤维的形成，与Ca的亲和力更强，控制钙球粒的形成。用抗硫酸肝素抗体染色人磨牙时，冠髓与根髓的免疫反应态度不同。认为这种观点与冠与根牙本质钙球石的形态和发育差异有关。这种反应在牙本质被固定的牙本质所在区域表现良好。钙球石存在于牙本质矿化前缘，认为钙球石的形成与硫酸肝素的定位有关。少