Chemical analysis and immunohistology research on non-collagen protein that takes part in the formation of calcospherites

参与钙球体形成的非胶原蛋白的化学分析和免疫组织学研究

基本信息

  • 批准号:
    13671914
  • 负责人:
  • 金额:
    $ 1.98万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
  • 财政年份:
    2001
  • 资助国家:
    日本
  • 起止时间:
    2001 至 2002
  • 项目状态:
    已结题

项目摘要

On the mammal, the mineralization of mantle dentin is formed with the mineralization of the matrix vesicle. But the mineralization of circumpulpal dentin is formed by the mineralization that relates to collagen fibers. The spherical calcospherites were observed in the predentin of crown dentin. In the apical region of root dentin, the calcospherites were not observed and the calcified collagen fibers were observed. The species specificity was detected in the development and distribution of calcospherites. As compared with in the cementum covered lingual dentin of continuously growing teeth such as rat and rabbit incisors, and in the cementum covered root dentin uncontinuously growing teeth such as humans and bovine incisors, the form and the size of calcospherites were greatly different. In the marsupial opossum dentin, the calcospherites were only observed at the cervical region of crown. In the reptile crocodilian dentin, the size of the calcospherites was smaller than that of mammal … More , and the fiber-like structure was observed on the surface of calcospherites. On the Ca/P ratio of calcospherites in human, bovine and opossum, the significant difference had been found between the crown and the root of dentin. The difference was not found on the Ca/P ratio of calcospherites in the continuously growing teeth such as rat and rabbit, and polyphyodont crocodilian. It is considered that the difference of the mechanisms of teeth formation related to that of calcospherites formation. With the results of the analytical electron microscopy, except the main element of calcium and phosphorus, elements of Mg S, K, and Al were detected in the stage of calcospherite formation. It was clarified that Mg especially had the key role the formation of the calcospherites among small amount elements from the observation of the analysis electron microscope A lot of Mg was detected in the first stage of the formation of calcospherites. Mg may contribute to physicochemical stability of the apatite crystal. It seems that Mg takes part in the nuclear formation on the initial stage of calcospherites formation. Moreover, it is considered that the localization of S means the existence of the proteoglycan that is non-collagen protein in the calcospherites. It is judged that S of the sulfuric acid radical in the proteoglycan was detected. The calcospherites of crocodilian were small and S was distributed from the cervical region to the root region of dentin. It was considered that the proteoglycan controlled the formation of calcospherites because it took part in the formation of collagen fibers and has closer affinity with Ca. When a human molar was stained with the antiheparan sulfate antibody, the immune reaction attitude was different between the crown pulp and the root pulp. It is considered that this opinion is related to the difference in the form and development of calcospherites between the crown and the root dentin. The reaction was seen well in the region with which dentin was being firmed in the predentin of crown. The calcospherites existed in the mineralization front of dentin, and it was suggested that the form of calcospherites was related to the localization of the heparan sulfate. Less
哺乳动物的牙本质矿化是随着基质囊泡矿化而形成的。而牙髓周围牙本质的矿化是由与胶原纤维有关的矿化形成的。在冠状牙本质前牙本质中观察到球状钙球粒。根牙本质顶端未见钙球粒形成,可见钙化的胶原纤维。在钙球石的发育和分布中发现了物种特异性。与连续生长的牙骨质覆盖舌牙本质(如大鼠、家兔)和非连续生长的牙骨质覆盖根牙本质(如人类、牛门牙)相比,钙球石的形态和大小有很大差异。在负鼠牙本质中,钙球粒仅在冠颈区可见。爬行动物的牙本质中,钙球粒的大小比哺乳动物的小,在钙球粒表面可见纤维状结构。在人、牛和负鼠钙球石的钙磷比上,牙本质的冠与根之间存在显著差异。大鼠、家兔等连续生长牙齿和多齿鳄的钙球石钙磷比无显著差异。认为牙齿形成机制的差异与钙球石形成机制的差异有关。分析电镜结果显示,钙球石形成阶段除主要元素钙、磷外,还检出镁、硫、钾、铝等元素。通过分析电镜观察,明确了微量元素中Mg对钙球石的形成起着关键作用,在钙球石形成的第一阶段就检测到大量的Mg。镁可能有助于磷灰石晶体的物理化学稳定性。镁元素似乎在钙球石形成初期参与了核的形成。此外,认为S的定位意味着钙球粒中存在非胶原蛋白的蛋白多糖。判断该蛋白多糖中检测到硫酸自由基S。鳄鱼的钙球粒小,S分布于牙本质的颈区至根区。认为蛋白多糖参与胶原纤维的形成,与Ca的亲和力更强,控制钙球粒的形成。用抗硫酸肝素抗体染色人磨牙时,冠髓与根髓的免疫反应态度不同。认为这种观点与冠与根牙本质钙球石的形态和发育差异有关。这种反应在牙本质被固定的牙本质所在区域表现良好。钙球石存在于牙本质矿化前缘,认为钙球石的形成与硫酸肝素的定位有关。少

项目成果

期刊论文数量(5)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
三島弘幸: "マイクロフォーカスX線CT装置を用いたヒト小臼歯の三次元再構築像の観察"日大口腔科学. 26・3. 244-248 (2000)
Hiroyuki Mishima:“使用微焦点X射线CT设备观察人体前磨牙的三维重建图像”日本大学口腔科学26・3(2000)。
  • DOI:
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  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Kakei, M.: "High-resolution electron microscopy of the crystallites of fossil enamels obtained from various geological ages"JOURNAL OF DENTAL RESEARCH. 80・6. 1560-1564 (2001)
Kakei, M.:“从不同地质时代获得的化石釉质微晶的高分辨率电子显微镜”《牙科研究杂志》80・6(2001)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Nagai, M.: "Invitro study of collagen coating of titanium implants for intial cell attachment"Dental material Journal. 21・3. 250-260 (2002)
Nagai, M.:“用于初始细胞附着的钛种植体胶原涂层的体外研究”牙科材料杂志 21・3(2002)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takashi Ozaki: "Incremental lines in the dentin of Alligator teeth before and after haching"Nihon University. Journal oral Science. 28・2. 143-151 (2002)
Takashi Ozaki:“鳄鱼牙齿牙本质的增量线”日本大学口腔科学杂志 28・2(2002)。
  • DOI:
  • 发表时间:
  • 期刊:
  • 影响因子:
    0
  • 作者:
  • 通讯作者:
Takeru Akazawa: "Neanderthal Burials Excavations of the Dederiyeh Cave Afrin, Syria"University of Tokyo Press Production Center. 394 (2002)
赤泽武:《叙利亚阿夫林德德里耶洞穴的尼安德特人墓葬发掘》东京大学出版社制作中心。
  • DOI:
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  • 影响因子:
    0
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MISHIMA Hiroyuki其他文献

MISHIMA Hiroyuki的其他文献

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{{ truncateString('MISHIMA Hiroyuki', 18)}}的其他基金

Clinical Genetics Knowledge Sharing System for Congenital Rare Disease Deep Phenotyping Overcoming the "30% limit" of Diagnosis
临床%20遗传学%20知识%20分享%20系统%20针对%20先天性%20罕见%20疾病%20深入%20表型%20克服%20%20"30%%20限制"%20的%20诊断
  • 批准号:
    18K07850
  • 财政年份:
    2018
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Control mechanism on structure, composition, and calcification of dentin and odontoblasts by time information transmitter melatonin
时间信息递质褪黑素对牙本质和成牙本质细胞结构、成分及钙化的控制机制
  • 批准号:
    15K11034
  • 财政年份:
    2015
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Effect of optic flow for passenger generatied by skilled/unskilled automobile driver.
熟练/不熟练的汽车驾驶员产生的光流对乘客的影响。
  • 批准号:
    26330319
  • 财政年份:
    2014
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Development of Integrated Annotation Workflows for Human Whole Genome Sequencing
人类全基因组测序集成注释工作流程的开发
  • 批准号:
    25430183
  • 财政年份:
    2013
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
The connection between the periodicity incremental lines of dentin and the secretion rhythm of melatonin of the signaling molecules of biological clock
牙本质周期性增量线与生物钟信号分子褪黑素分泌节律的关系
  • 批准号:
    23592727
  • 财政年份:
    2011
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Genomics Approach to orofacial clefts focusing theubiquitin-proteasome system
关注泛素-蛋白酶体系统的口面裂基因组学方法
  • 批准号:
    23791230
  • 财政年份:
    2011
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
Individual cervical cancer susceptibility and genomic structural variations of the defensin genes
个体宫颈癌易感性和防御素基因的基因组结构变异
  • 批准号:
    21791566
  • 财政年份:
    2009
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Young Scientists (B)
The relationship between the periodicity of incremental lines in the tooth dentin and a biological clock : Regulation by melatonin
牙齿牙本质增量线的周期性与生物钟之间的关系:褪黑激素的调节
  • 批准号:
    20592168
  • 财政年份:
    2008
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
Perception of body orientation by dynamic touch, and its consequences for posture control.
通过动态触摸感知身体方向及其对姿势控制的影响。
  • 批准号:
    20500243
  • 财政年份:
    2008
  • 资助金额:
    $ 1.98万
  • 项目类别:
    Grant-in-Aid for Scientific Research (C)
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