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Chemical analysis and immunohistology research on non-collagen protein that takes part in the formation of calcospherites

参与钙球体形成的非胶原蛋白的化学分析和免疫组织学研究

基本信息

批准号：
13671914
负责人：
MISHIMA Hiroyuki
金额：
$ 1.98万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13671914/
关键词：
Globular calcification Linear calcification Calcospherite Orientation of apatite crystal Collagen fibers Mode of calcification Crown dentin Root deuntin プロテオグリカン成長線

项目摘要

On the mammal, the mineralization of mantle dentin is formed with the mineralization of the matrix vesicle. But the mineralization of circumpulpal dentin is formed by the mineralization that relates to collagen fibers. The spherical calcospherites were observed in the predentin of crown dentin. In the apical region of root dentin, the calcospherites were not observed and the calcified collagen fibers were observed. The species specificity was detected in the development and distribution of calcospherites. As compared with in the cementum covered lingual dentin of continuously growing teeth such as rat and rabbit incisors, and in the cementum covered root dentin uncontinuously growing teeth such as humans and bovine incisors, the form and the size of calcospherites were greatly different. In the marsupial opossum dentin, the calcospherites were only observed at the cervical region of crown. In the reptile crocodilian dentin, the size of the calcospherites was smaller than that of mammal … More , and the fiber-like structure was observed on the surface of calcospherites. On the Ca/P ratio of calcospherites in human, bovine and opossum, the significant difference had been found between the crown and the root of dentin. The difference was not found on the Ca/P ratio of calcospherites in the continuously growing teeth such as rat and rabbit, and polyphyodont crocodilian. It is considered that the difference of the mechanisms of teeth formation related to that of calcospherites formation. With the results of the analytical electron microscopy, except the main element of calcium and phosphorus, elements of Mg S, K, and Al were detected in the stage of calcospherite formation. It was clarified that Mg especially had the key role the formation of the calcospherites among small amount elements from the observation of the analysis electron microscope A lot of Mg was detected in the first stage of the formation of calcospherites. Mg may contribute to physicochemical stability of the apatite crystal. It seems that Mg takes part in the nuclear formation on the initial stage of calcospherites formation. Moreover, it is considered that the localization of S means the existence of the proteoglycan that is non-collagen protein in the calcospherites. It is judged that S of the sulfuric acid radical in the proteoglycan was detected. The calcospherites of crocodilian were small and S was distributed from the cervical region to the root region of dentin. It was considered that the proteoglycan controlled the formation of calcospherites because it took part in the formation of collagen fibers and has closer affinity with Ca. When a human molar was stained with the antiheparan sulfate antibody, the immune reaction attitude was different between the crown pulp and the root pulp. It is considered that this opinion is related to the difference in the form and development of calcospherites between the crown and the root dentin. The reaction was seen well in the region with which dentin was being firmed in the predentin of crown. The calcospherites existed in the mineralization front of dentin, and it was suggested that the form of calcospherites was related to the localization of the heparan sulfate. Less

在哺乳动物身上，外套质牙本质的矿化是随着基质小泡的矿化而形成的。而牙髓周围牙本质的矿化是由与胶原纤维有关的矿化形成的。冠牙本质前牙本质内可见球状钙球。在根部牙本质的根尖区，未见钙化颗粒，可见钙化的胶原纤维。钙球石的发育和分布具有种属特异性。与大鼠、兔门牙等持续生长的牙本质以及人、牛门牙等非连续生长牙的牙骨质覆盖根部相比，钙球的形态和大小有很大的不同。在有袋负鼠牙本质中，钙球仅见于牙冠颈部。在爬行动物鳄鱼的牙本质中，钙球的大小小于哺乳动物…的大小此外，在方解石表面还观察到纤维状结构。在人、牛和负鼠的钙球钙磷比上，牙本质的冠和根之间有显著差异。连续生长的大鼠、兔、多齿鳄鱼等牙齿中钙球的钙磷比无差异。认为牙齿形成机制的差异与方解石的形成机制有关。分析电子显微镜结果表明，在方解石形成阶段，除主要元素钙、磷外，还含有镁、S、钾、铝等元素。分析电子显微镜观察表明，在少量元素中，镁对钙球石的形成起着关键作用。在钙球石形成的第一阶段，检测到大量的镁。镁可能对磷灰石晶体的物理化学稳定性有贡献。镁似乎参与了方解石形成初期的成核过程。此外，认为S的定位意味着在钙球体内存在蛋白多糖，即非胶原蛋白。判断检测到蛋白多糖中的硫酸根S。鳄鱼的钙球较小，S分布于牙本质的颈部至根部。认为蛋白多糖参与了胶原纤维的形成，并与钙离子亲和力较强，从而控制了钙球的形成。用抗硫酸肝素抗体对人磨牙进行染色，发现根髓和冠髓的免疫反应态度不同。认为这一观点与牙冠、根部牙本质中钙球的形成和发育不同有关。在牙冠前牙本质与牙本质结合的区域，反应很明显。钙球存在于牙本质矿化前沿，提示钙球的形成与硫酸乙酰肝素的定位有关。较少