Development and application of immobilized receptor with the object of screening of drugs activator.

以筛选药物激活剂为目的的固定化受体的开发和应用。

• 批准号: 13672267
• 负责人: MATSUNAGA Hisami
• 金额: $ 2.3万
• 依托单位: Mukogawa Women's University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2003
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672267/
• 关键词: chicken α_1-acid glycoprotein; MALDI-TOF MS; disulfide bond; sugar chain; 糖タンパク質; 固定化カラム; 光学分割; 光学認識機構; ジスルフィド結合; アミノ酸配列; 医薬品活性体; 糖タンパク質の遺伝子組み換え

Recently, we reported the amino acid sequence of chicken α_1-acid glycoprotein(chicken α_1-AGP)(Biochem.Biophys.Res.Commun.295(2002)587-590). In this study, we identify disulfide bonds and site-specific glycosylation in chicken α_1-AGP using tryptic digests of carbamidomethylated, chicken α_1-AGP, carbamidomethylated, completely deglycosylated chicken α_1-AGP(cd-α_1-AGP), and non-reduced, denatured cd-α_1-AGP by matrix-assisted laser desorption ionization time-of-flight mass spectrometry(MALDI-TOF-MS). Based on the existence of the peptides detected at m/z 3037.4(amino acid sequence 69-76 plus 161-183) and 3453.3(amino acid sequence 69-80 plus 161-183), the location of two disulfide bonds of chicken α_1-AGP was determined to be Cys 6-Cys 146 and Cys 73-Cys 163. Furthermore, it was found that Asn 16,70,77 and 87 were fully glycosylated, and that Asn 62 was partially glycosylated.

最近，我们报道了鸡α_1-酸性糖蛋白（chicken α_1-AGP）的氨基酸序列（Biochem.Bioprotein.Res.Commun.295（2002）587-590）。本研究采用胰蛋白酶消化法对鸡α_1-AGP、完全去糖基化的鸡α_1-AGP（cd-α_1-AGP）和非还原变性的鸡α_1-AGP进行了基质辅助激光解吸电离飞行时间质谱（MALDI-TOF-MS）分析。根据m/z 3037.4（氨基酸序列69-76加161-183）和3453.3（氨基酸序列69-80加161-183）处检测到的肽段的存在，确定鸡α 1-AGP的两个二硫键的位置为Cys 6-Cys 146和Cys 73-Cys 163。Asn 16、70、77和87为完全糖基化，Asn 62为部分糖基化.

项目成果

Yutaka Sadakane: "Protein domein of chicken α_1-acid glycoprotein is responsible for chiral recognition"Biochemical and Biophysical Research Communications. 295. 587-590 (2002)

Yutaka Sadakane：“鸡α_1-酸性糖蛋白的蛋白质结构域负责手性识别”《生物化学和生物物理研究通讯》295. 587-590 (2002)。

Yutaka Sadakane: "Protein domain of chicken α_1-acid glycoprotein is responsible for chiral recognition."B.B.R.C.. 295. 587-590 (2002)

Yutaka Sadakane：“鸡 α_1-酸性糖蛋白的蛋白质结构域负责手性识别。”B.B.R.C.. 295. 587-590 (2002)

Yutaka Sadakane: "Protein domein of chicken α1-acid glycoprotein is responsible for chiral recognition"Biochemical and Biophysical Research Communications. 295. 587-590 (2002)

Yutaka Sadakane：“鸡α1-酸性糖蛋白的蛋白质结构域负责手性识别”《生物化学和生物物理研究通讯》295. 587-590 (2002)。

Matsunaga Hisami: "HPLC Chiral Stationary Phases Produced with Isolated Human Serum Albumin Fragments"Analytical Sciences. 18. 27-30 (2002)

Matsunaga Hisami：“用分离的人血清白蛋白片段产生的 HPLC 手性固定相”分析科学。

Hisami Matsunaga: "Separation of basic drug enantiomers by capillary electrophoresis using chicken α_1-acid glycoprotein : Insight into chiral recognition mechanism."Electrophoresis. 24. 2442-2447 (2003)

Hisami Matsunaga：“使用鸡 α_1-酸性糖蛋白通过毛细管电泳分离碱性药物对映体：洞察手性识别机制。”电泳 24. 2442-2447 (2003)